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JCS ePress online publication date 1 Jun 2004
doi: 10.1242/jcs.01086

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Research Article

Binding of 14-3-3{beta} but not 14-3-3{sigma} controls the cytoplasmic localization of CDC25B: binding site preferences of 14-3-3 subtypes and the subcellular localization of CDC25B


Sanae Uchida, Akiko Kuma, Motoaki Ohtsubo, Mari Shimura, Masato Hirata, Hitoshi Nakagama, Tsukasa Matsunaga, Yukihito Ishizaka, and Katsumi Yamashita*
* Author for correspondence (e-mail: katsumi{at}kenroku.kanazawa-u.ac.jp)

The dual specificity phosphatase CDC25B positively controls the G2-M transition by activating CDK1/cyclin B. The binding of 14-3-3 to CDC25B has been shown to regulate the subcellular redistribution of CDC25B from the nucleus to the cytoplasm and may be correlated with the G2 checkpoint. We used a FLAG-tagged version of CDC25B to study the differences among the binding sites for the 14-3-3 subtypes, 14-3-3{beta}, 14-3-3{epsilon} and 14-3-3{sigma}, and the relationship between subtype binding and the subcellular localization of CDC25B. All three subtypes were found to bind to CDC25B. Site-directed mutagenesis studies revealed that 14-3-3{beta} bound exclusively near serine-309 of CDC25B1, which is within a potential consensus motif for 14-3-3 binding. By contrast, 14-3-3{sigma} bound preferentially to a site around serine-216, and the presence of serine-137 and -309 enhanced the binding. In addition to these binding-site differences, we found that the binding of 14-3-3{beta} drove CDC25B to the cytoplasm and that mutation of serine-309 to alanine completely abolished the cytoplasmic localization of CDC25B. However, co-expression of 14-3-3{sigma} and CDC25B did not affect the subcellular localization of CDC25B. Furthermore, serine-309 of CDC25B was sufficient to produce its cytoplasmic distribution with co-expression of 14-3-3{beta}, even when other putative 14-3-3 binding sites were mutated. 14-3-3{epsilon} resembled 14-3-3{beta} with regard to its binding to CDC25B and the control of CDC25B subcellular localization. The results of the present study indicate that two 14-3-3 subtypes can control the subcellular localization of CDC25B by binding to a specific site and that 14-3-3{sigma} has effects on CDC25B other than the control of its subcellular localization.
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