|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search | |||||
The fully linked HTML version of this article has now been published.
The biflagellate unicellular green alga Chlamydomonas reinhardtii is a classic model organism for the analysis of flagella and their organizers, the basal bodies. In this cell, the two flagella-bearing basal bodies, along with two probasal bodies and an array of fibers and microtubules, form a complex organelle called the basal apparatus. The ultrastructure of the basal apparatus was analysed in detail by serial thin-section electron microscopy of isolated cytoskeletons and several newly discovered features are described, including a marker for the rotational asymmetry inherent in the basal bodies and probasal bodies. In addition, the complex three-dimensional basal apparatus ultrastructure is resolved and illustrated, including the attachment sites of all basal apparatus elements to specific microtubular triplets of the basal bodies and probasal bodies. These data will facilitate both the localization of novel basal apparatus proteins and the analysis of mutants and RNA interference cells with only subtle defects in basal apparatus ultrastructure. The early harbinger of radial asymmetry described here could play a crucial role during basal body maturation by orienting the asymmetric attachment of the various associated fibers and therefore might define the orientation of the basal bodies and, ultimately, the direction of flagellar beating.
This article has been cited by other articles:
JCS ePress
online publication date 11 May 2004
doi: 10.1242/jcs.01120
This Article 
Full Text (PDF)
All Versions of this Article:
jcs.01120v1
117/13/2663
most recent
Alert me when this article is cited
Alert me if a correction is posted
Services
Email this article to a friend
Similar articles in this journal
Similar articles in PubMed
Alert me to new issues of the journal
Download to citation manager
Citing Articles
Citing Articles via HighWire
Citing Articles via Google Scholar
Google Scholar
Articles by Geimer, S.
Articles by Melkonian, M.
Search for Related Content
PubMed
PubMed Citation
Articles by Geimer, S.
Articles by Melkonian, M.
Social Bookmarking
What's this?
Research Article
The ultrastructure of the Chlamydomonas reinhardtii basal apparatus: identification of an early marker of radial asymmetry inherent in the basal body
* Author for correspondence (e-mail: stefan.geimer{at}uni-koeln.de)
CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati Twitter What's this?
S. Lacomble, S. Vaughan, C. Gadelha, M. K. Morphew, M. K. Shaw, J. R. McIntosh, and K. Gull
Three-dimensional cellular architecture of the flagellar pocket and associated cytoskeleton in trypanosomes revealed by electron microscope tomography
J. Cell Sci.,
April 15, 2009;
122(8):
1081 - 1090.
[Abstract]
[Full Text]
[PDF]
B. P. Piasecki and C. D. Silflow
The UNI1 and UNI2 Genes Function in the Transition of Triplet to Doublet Microtubules between the Centriole and Cilium in Chlamydomonas
Mol. Biol. Cell,
January 1, 2009;
20(1):
368 - 378.
[Abstract]
[Full Text]
[PDF]
B. P. Piasecki, M. LaVoie, L.-W. Tam, P. A. Lefebvre, and C. D. Silflow
The Uni2 Phosphoprotein is a Cell Cycle regulated Component of the Basal Body Maturation Pathway in Chlamydomonas reinhardtii
Mol. Biol. Cell,
January 1, 2008;
19(1):
262 - 273.
[Abstract]
[Full Text]
[PDF]
J. Xu, A. Van Keymeulen, N. M. Wakida, P. Carlton, M. W. Berns, and H. R. Bourne
Polarity reveals intrinsic cell chirality
PNAS,
May 29, 2007;
104(22):
9296 - 9300.
[Abstract]
[Full Text]
[PDF]
S. Geimer and M. Melkonian
Centrin Scaffold in Chlamydomonas reinhardtii Revealed by Immunoelectron Microscopy
Eukaryot. Cell,
July 1, 2005;
4(7):
1253 - 1263.
[Abstract]
[Full Text]
[PDF]
© The Company of Biologists Ltd 2004