The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 25 May 2004
doi: 10.1242/jcs.01155
Research Article
The nucleolus is involved in mRNA export from the nucleus in fission yeast
Takashi Ideue,
Abul Kalam Azad,
Jun-ichi Yoshida,
Tadao Matsusaka,
Mitsuhiro Yanagida,
Yasumi Ohshima,
and
Tokio Tani*
* Author for correspondence (e-mail: ttani{at}sci.kumamoto-u.ac.jp)
To elucidate the mechanism of mRNA export from the nucleus, we isolated five novel temperature-sensitive mutants (ptr7 to ptr11) that accumulate poly(A)+ RNA in the nuclei at the nonpermissive temperature in Schizosaccharomyces pombe. Of those, the ptr11 mutation was found in the top2+ gene encoding DNA topoisomerase II. In addition to the nuclear accumulation of poly(A)+ RNA, ptr11 exhibited the cut (cell untimely torn) phenotype at the nonpermissive temperature, like the previously isolated mutant, ptr4. In these two mutants, cytokinesis occurred without prior nuclear division, resulting in cleavage of the undivided nuclei by the septum. To investigate the relationship between mRNA export defects and the cut phenotype observed in ptr4 and ptr11, we analyzed 11 other mutants displaying the cut phenotype and found that all these tested mutants accumulate poly(A)+ mRNA in the aberrantly cleaved nuclei. Interestingly, nuclear accumulation of poly(A)+ mRNA was observed only in the anucleolate nuclei produced by aberrant cytokinesis. In addition, nuc1, the S. pombe mutant exhibiting a collapsed nucleolus, trapped poly(A)+ mRNA in the nucleolar region at the nonpermissive temperature. In ptr11 and nuc1, mRNA transcribed from the intron-containing TBP gene showed nuclear accumulation, but not transcripts from the intron-less TBP cDNA, suggesting that the export pathway differs between the spliced and unspliced TBP mRNAs. These findings support the notion that a subset of mRNAs in yeast is exported from the nucleus through transient association with the nucleolus.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
Y. Guo, M. Su, M. A. McNutt, and J. Gu
Expression and Distribution of Cystic Fibrosis Transmembrane Conductance Regulator in Neurons of the Human Brain
J. Histochem. Cytochem.,
December 1, 2009;
57(12):
1113 - 1120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Blanco, L. Rausell, B. Aguado, M. Perez-Alonso, and R. Artero
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Nucleic Acids Res.,
September 1, 2009;
37(17):
e116 - e116.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. H. Kim, O. A. Koroleva, D. Lewandowska, A. F. Pendle, G. P. Clark, C. G. Simpson, P. J. Shaw, and J. W.S. Brown
Aberrant mRNA Transcripts and the Nonsense-Mediated Decay Proteins UPF2 and UPF3 Are Enriched in the Arabidopsis Nucleolus
PLANT CELL,
July 1, 2009;
21(7):
2045 - 2057.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-W. Park, A. M. Parrott, D. T. Fritz, Y. Park, M. B. Mathews, and C.-G. Lee
Regulation of the catalytic function of topoisomerase II alpha through association with RNA
Nucleic Acids Res.,
November 1, 2008;
36(19):
6080 - 6090.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Thomsen, C. Saguez, T. Nasser, and T. H. Jensen
General, rapid, and transcription-dependent fragmentation of nucleolar antigens in S. cerevisiae mRNA export mutants
RNA,
April 1, 2008;
14(4):
706 - 716.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mizuki, T. Namiki, H. Sato, H. Furukawa, T. Matsusaka, Y. Ohshima, R. Ishibashi, T. Andoh, and T. Tani
Participation of XPB/Ptr8p, a component of TFIIH, in nucleocytoplasmic transport of mRNA in fission yeast.
Genes Cells,
January 1, 2007;
12(1):
35 - 47.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Boyne and A. Whitehouse
Nucleolar trafficking is essential for nuclear export of intronless herpesvirus mRNA
PNAS,
October 10, 2006;
103(41):
15190 - 15195.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. Negi and M. O. J. Olson
Effects of interphase and mitotic phosphorylation on the mobility and location of nucleolar protein B23
J. Cell Sci.,
September 1, 2006;
119(17):
3676 - 3685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Z. Win, S. Draper, R. L. Read, J. Pearce, C. J. Norbury, and S.-W. Wang
Requirement of Fission Yeast Cid14 in Polyadenylation of rRNAs.
Mol. Cell. Biol.,
March 1, 2006;
26(5):
1710 - 1721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Hirose, M. Mukai, A. Shimada, H. Nishitani, Y. Shibata, and T. Nishimoto
Loss of RanGEF/Pim1 activity abolishes the orchestration of Ran-mediated mitotic cellular events in S. pombe
Genes Cells,
January 1, 2006;
11(1):
29 - 46.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2004
