The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 20 Jul 2004
doi: 10.1242/jcs.01239
Research Article
The yeast dynamin-related GTPase Vps1p functions in the organization of the actin cytoskeleton via interaction with Sla1p
Xianwen Yu
and
Mingjie Cai*
* Author for correspondence (e-mail: mcbcaimj{at}imcb.nus.edu.sg)
Recent studies have suggested that the function of the large GTPase dynamin in endocytosis in mammalian cells may comprise a modulation of actin cytoskeleton. The role of dynamin in actin cytoskeleton organization in the yeast Saccharomyces cerevisiae has remained undefined. In this report, we found that one of the yeast dynamin-related proteins, Vps1p, is required for normal actin cytoskeleton organization. At both permissive and non-permissive temperatures, the vps1 mutants exhibited various degrees of phenotypes commonly associated with actin cytoskeleton defects: depolarized and aggregated actin structures, hypersensitivity to the actin cytoskeleton toxin latrunculin-A, randomized bud site selection and chitin deposition, and impaired efficiency in the internalization of membrane receptors. Over-expression of the GTPase mutants of vps1 also led to actin abnormalities. Consistent with these actin-related defects, Vps1p was found to interact physically, and partially co-localize, with the actin-regulatory protein Sla1p. The normal cellular localization of Sla1p required Vps1p and could be altered by over-expression of a region of Vps1p that was involved in the interaction with Sla1p. The same region also promoted mis-sorting of the vacuolar protein carboxypeptidase Y upon over-expression. These findings suggest that the functions of the dynamin-related protein Vps1p in actin cytoskeleton dynamics and vacuolar protein sorting are probably related to each other.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
K. Mitsui, K. Hatakeyama, M. Matsushita, and H. Kanazawa
Saccharomyces cerevisiae Na+/H+ Antiporter Nha1p Associates with Lipid Rafts and Requires Sphingolipid for Stable Localization to the Plasma Membrane
J. Biochem.,
June 1, 2009;
145(6):
709 - 720.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Konopka, S. K. Backues, and S. Y. Bednarek
Dynamics of Arabidopsis Dynamin-Related Protein 1C and a Clathrin Light Chain at the Plasma Membrane
PLANT CELL,
May 1, 2008;
20(5):
1363 - 1380.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F.-Z. Idrissi, H. Grotsch, I. M. Fernandez-Golbano, C. Presciatto-Baschong, H. Riezman, and M.-I. Geli
Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane
J. Cell Biol.,
March 24, 2008;
180(6):
1219 - 1232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Welsh, A. H. Y. Tong, C. Boone, O. N. Jensen, and S. Otte
Genetic and molecular interactions of the Erv41p-Erv46p complex involved in transport between the endoplasmic reticulum and Golgi complex
J. Cell Sci.,
November 15, 2006;
119(22):
4730 - 4740.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kuravi, S. Nagotu, A. M. Krikken, K. Sjollema, M. Deckers, R. Erdmann, M. Veenhuis, and I. J. van der Klei
Dynamin-related proteins Vps1p and Dnm1p control peroxisome abundance in Saccharomyces cerevisiae
J. Cell Sci.,
October 1, 2006;
119(19):
3994 - 4001.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. J. Vizeacoumar, W. N. Vreden, M. Fagarasanu, G. A. Eitzen, J. D. Aitchison, and R. A. Rachubinski
The Dynamin-like Protein Vps1p of the Yeast Saccharomyces cerevisiae Associates with Peroxisomes in a Pex19p-dependent Manner
J. Biol. Chem.,
May 5, 2006;
281(18):
12817 - 12823.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Ren, P. Vajjhala, J. S. Lee, B. Winsor, and A. L. Munn
The BAR Domain Proteins: Molding Membranes in Fission, Fusion, and Phagy
Microbiol. Mol. Biol. Rev.,
March 1, 2006;
70(1):
37 - 120.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2004
