spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 17 Aug 2004
doi: 10.1242/jcs.01308

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01308v1
117/19/4423    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nesic, D.
Right arrow Articles by Krämer, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nesic, D.
Right arrow Articles by Krämer, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

A role for Cajal bodies in the final steps of U2 snRNP biogenesis


Dobrila Nesic, Goranka Tanackovic, and Angela Krämer*
* Author for correspondence (e-mail: angela.kraemer{at}cellbio.unige.ch)

The biogenesis of Sm-type small nuclear ribonucleoproteins (snRNPs) involves the export of newly transcribed small nuclear RNAs (snRNAs) to the cytoplasm, assembly with seven common proteins and modification at the 5' and 3' termini. Binding of snRNP-specific proteins and snRNA modification complete the maturation process. This is thought to occur after reimport of the core snRNPs into the nucleus. The heterotrimeric splicing factor SF3a converts a pre-mature 15S U2 snRNP into the functional 17S particle. To analyze cellular aspects of this process, we studied domains in SF3a60 and SF3a66 that are required for their localization to nuclear speckles. Regions in SF3a60 and SF3a66 that mediate the binding to SF3a120 are necessary for nuclear import of the proteins, suggesting that the SF3a heterotrimer forms in the cytoplasm. SF3a60 and SF3a66 deleted for zinc finger domains required for the incorporation of SF3a into the U2 snRNP are nuclear, indicating that the 17S U2 snRNP is assembled in the nucleus. However, these proteins show an aberrant nuclear distribution. Endogenous SF3a subunits colocalize with U2 snRNP in nuclear speckles, but cannot be detected in Cajal bodies, unlike core U2 snRNP components. By contrast, SF3a60 and SF3a66 lacking the zinc finger domains accumulate in Cajal bodies and are diffusely distributed in the cytoplasm, suggesting a function for Cajal bodies in the final maturation of the U2 snRNP.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
S. M. Hearst, A. S. Gilder, S. S. Negi, M. D. Davis, E. M. George, A. A. Whittom, C. G. Toyota, A. Husedzinovic, O. J. Gruss, and M. D. Hebert
Cajal-body formation correlates with differential coilin phosphorylation in primary and transformed cell lines
J. Cell Sci., June 1, 2009; 122(11): 1872 - 1881.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. B. Patel and M. Bellini
The assembly of a spliceosomal small nuclear ribonucleoprotein particle
Nucleic Acids Res., November 1, 2008; 36(20): 6482 - 6493.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Stanek, J. Pridalova-Hnilicova, I. Novotny, M. Huranova, M. Blazikova, X. Wen, A. K. Sapra, and K. M. Neugebauer
Spliceosomal Small Nuclear Ribonucleoprotein Particles Repeatedly Cycle through Cajal Bodies
Mol. Biol. Cell, June 1, 2008; 19(6): 2534 - 2543.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
G. B. Gonsalvez, K. Praveen, A. J. Hicks, L. Tian, and A. G. Matera
Sm protein methylation is dispensable for snRNP assembly in Drosophila melanogaster
RNA, May 1, 2008; 14(5): 878 - 887.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Sleeman
A regulatory role for CRM1 in the multi-directional trafficking of splicing snRNPs in the mammalian nucleus
J. Cell Sci., May 1, 2007; 120(9): 1540 - 1550.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Klingauf, D. Stanek, and K. M. Neugebauer
Enhancement of U4/U6 Small Nuclear Ribonucleoprotein Particle Association in Cajal Bodies Predicted by Mathematical Modeling
Mol. Biol. Cell, December 1, 2006; 17(12): 4972 - 4981.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Wang, E. O. Gracheva, J. Richmond, T. Kawano, J. M. Couto, J. A. Calarco, V. Vijayaratnam, Y. Jin, and M. Zhen
The C2H2 zinc-finger protein SYD-9 is a putative posttranscriptional regulator for synaptic transmission
PNAS, July 5, 2006; 103(27): 10450 - 10455.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Collier, A. Pendle, K. Boudonck, T. van Rij, L. Dolan, and P. Shaw
A Distant Coilin Homologue Is Required for the Formation of Cajal Bodies in Arabidopsis
Mol. Biol. Cell, July 1, 2006; 17(7): 2942 - 2951.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
B. Renvoise, K. Khoobarry, M.-C. Gendron, C. Cibert, L. Viollet, and S. Lefebvre
Distinct domains of the spinal muscular atrophy protein SMN are required for targeting to Cajal bodies in mammalian cells
J. Cell Sci., February 15, 2006; 119(4): 680 - 692.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Sun, H. Xu, S. H. Subramony, and M. D. Hebert
Interactions between Coilin and PIASy partially link Cajal bodies to PML bodies
J. Cell Sci., November 1, 2005; 118(21): 4995 - 5003.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. K. Ospina, G. B. Gonsalvez, J. Bednenko, E. Darzynkiewicz, L. Gerace, and A. G. Matera
Cross-Talk between Snurportin1 Subdomains
Mol. Biol. Cell, October 1, 2005; 16(10): 4660 - 4671.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
G. Tanackovic and A. Kramer
Human Splicing Factor SF3a, but Not SF1, Is Essential for Pre-mRNA Splicing In Vivo
Mol. Biol. Cell, March 1, 2005; 16(3): 1366 - 1377.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004