The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 17 Aug 2004
doi: 10.1242/jcs.01356
Research Article
Regulation of cytoplasmic stress granules by apoptosis-inducing factor
Céline Candé,
Nicola Vahsen,
Didier Métivier,
Hélène Tourrière,
Karim Chebli,
Carmen Garrido,
Jamal Tazi,
and
Guido Kroemer*
* Author for correspondence (e-mail: kroemer{at}igr.fr)
Stress granules (SG) are dynamic cytoplasmic foci in which stalled translation initiation complexes accumulate. In conditions of acute cellular redox, stress cells manipulated to lose the expression of apoptosis-inducing factor (AIF) nucleate SG signature proteins (e.g. TIA-1, PABP1) more efficiently than AIF-positive controls. AIF also inhibited SG formation induced by the RasGAP-associated endoribonuclease G3BP. Retransfection of mouse AIF into cells subjected to human AIF-specific siRNA revealed that only AIF imported into mitochondria could repress SGs and that redox-active domains of AIF, which are dispensable for its apoptogenic action, were required for SG inhibition. In response to oxidative stress, AIF-negative cells were found to deplete non-oxidized glutathione more rapidly than AIF-expressing cells. Exogenous supplementation of glutathione inhibited SG formation elicited by arsenate or G3BP. Together, these data suggest that the oxidoreductase function of AIF is required for the maintenance of glutathione levels in stress conditions and that glutathione is a major regulator of SG.
This article has been cited by other articles:
|
|
|
|
 
I. Y. Churbanova and I. F. Sevrioukova
Redox-dependent Changes in Molecular Properties of Mitochondrial Apoptosis-inducing Factor
J. Biol. Chem.,
February 29, 2008;
283(9):
5622 - 5631.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. V. Ilieva, V. Ayala, M. Jove, E. Dalfo, D. Cacabelos, M. Povedano, M. J. Bellmunt, I. Ferrer, R. Pamplona, and M. Portero-Otin
Oxidative and endoplasmic reticulum stress interplay in sporadic amyotrophic lateral sclerosis
Brain,
December 1, 2007;
130(12):
3111 - 3123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. P.M. van Empel, A. T. Bertrand, R. J. van Oort, R. van der Nagel, M. Engelen, H. V. van Rijen, P. A. Doevendans, H. J. Crijns, S. L. Ackerman, W. Sluiter, et al.
EUK-8, a Superoxide Dismutase and Catalase Mimetic, Reduces Cardiac Oxidative Stress and Ameliorates Pressure Overload-Induced Heart Failure in the Harlequin Mouse Mutant
J. Am. Coll. Cardiol.,
August 15, 2006;
48(4):
824 - 832.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Delettre, V. J. Yuste, R. S. Moubarak, M. Bras, N. Robert, and S. A. Susin
Identification and Characterization of AIFsh2, a Mitochondrial Apoptosis-inducing Factor (AIF) Isoform with NADH Oxidase Activity
J. Biol. Chem.,
July 7, 2006;
281(27):
18507 - 18518.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Zekri, K. Chebli, H. Tourriere, F. C. Nielsen, T. V. O. Hansen, A. Rami, and J. Tazi
Control of Fetal Growth and Neonatal Survival by the RasGAP-Associated Endoribonuclease G3BP
Mol. Cell. Biol.,
October 1, 2005;
25(19):
8703 - 8716.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. P.M. van Empel, A. T. Bertrand, R. van der Nagel, S. Kostin, P. A. Doevendans, H. J. Crijns, E. de Wit, W. Sluiter, S. L. Ackerman, and L. J. De Windt
Downregulation of Apoptosis-Inducing Factor in Harlequin Mutant Mice Sensitizes the Myocardium to Oxidative Stress-Related Cell Death and Pressure Overload-Induced Decompensation
Circ. Res.,
June 24, 2005;
96(12):
e92 - e101.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2004
