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JCS ePress
online publication date 31 Aug 2004
doi: 10.1242/jcs.01371
Research Article
Light-dependent subcellular translocation of Gq
in Drosophila photoreceptors is facilitated by the photoreceptor-specific myosin III NINAC
Michelle A. Cronin,
Fengqiu Diao,
and
Susan Tsunoda*
* Author for correspondence (e-mail: tsunoda{at}bu.edu)
We examine the light-dependent subcellular translocation of the visual Gq
protein between the signaling compartment, the rhabdomere and the cell body in Drosophila photoreceptors. We characterize the translocation of Gq and provide the first evidence implicating the involvement of the photoreceptor-specific myosin III NINAC in Gq transport. Translocation of Gq from the rhabdomere to the cell body is rapid, taking less than 5 minutes. Higher light intensities increased the quantity of Gq translocated out of the rhabdomeres from 20% to 75%, consistent with a mechanism for light adaptation. We demonstrate that translocation of Gq requires rhodopsin, but none of the known downstream phototransduction components, suggesting that the signaling pathway triggering translocation occurs upstream of Gq. Finally, we show that ninaC mutants display a significantly reduced rate of Gq transport from the cell body to the rhabdomere, suggesting that NINAC might function as a light-dependent plus-end motor involved in the transport of Gq.
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