spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 18 Jan 2005
doi: 10.1242/jcs.01402

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01402v1
118/3/643    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nohe, A.
Right arrow Articles by Petersen, N. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nohe, A.
Right arrow Articles by Petersen, N. O.

Research Article

Dynamics and interaction of caveolin-1 isoforms with BMP-receptors


Anja Nohe, Eleonora Keating, T. Michael Underhill, Petra Knaus, and Nils O. Petersen*
* Author for correspondence (e-mail: petersen{at}uwo.ca)

Caveolae are small invaginations of the cell membrane that are thought to play a role in important physiological functions such as cell surface signaling, endocytosis and intracellular cholesterol transport. Caveolin-1 is a key protein in these domains and contributes to the organization of cholesterol and saturated lipids within these vesicular invaginations of the plasma membrane. Caveolae are thought to be involved in the signaling of tyrosine kinase receptors and serine threonine receptors. In this article we focus on the involvement of caveolae in the signal transduction of bone morphogenetic proteins (BMPs). BMPs play important roles during embryonic development and especially in chondrogenesis, osteogenesis, neurogenesis and hematopoiesis. The initiation of the signal tranduction starts by the binding of a BMP to a corresponding set of BMP receptors.

Using image cross-correlation spectroscopy, we show that the BMP receptors BRIa and BRII colocalize with caveolin-1 isoforms {alpha} and {beta} on the cell surface. BRIa colocalizes predominantly with the caveolin-1 {alpha} isoform. Coexpression of BRII leads to a redistribution of BRIa into domains enriched in caveolin-1 {beta}. After stimulation with BMP-2, BRIa moves back into the region with caveolin-1 {alpha}. BRII is expressed in regions enriched in caveolin-1 {alpha} and {beta}. Stimulation of cells with BMP-2 leads to a redistribution of BRII into domains enriched in caveolin-1 {alpha}. Immunoprecipitation studies using transfected COS-7 cells indicate that BRII binds to caveolin-1 {alpha} and {beta}. The binding of BRII to caveolin-1 was verified using A431 cells. Stimulation of starved A431 cells with BMP-2 lead to a release of caveolin-1 from the BMP receptors. We show further that the caveolin-1 {beta} isoform inhibits BMP signaling whereas the {alpha} isoform does not.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. A. Maniatis, V. Shinin, D. E. Schraufnagel, S. Okada, S. M. Vogel, A. B. Malik, and R. D. Minshall
Increased pulmonary vascular resistance and defective pulmonary artery filling in caveolin-1-/- mice
Am J Physiol Lung Cell Mol Physiol, May 1, 2008; 294(5): L865 - L873.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
J. F. Santibanez, F. J. Blanco, E. M. Garrido-Martin, F. Sanz-Rodriguez, M. A. del Pozo, and C. Bernabeu
Caveolin-1 interacts and cooperates with the transforming growth factor-{beta} type I receptor ALK1 in endothelial caveolae
Cardiovasc Res, March 1, 2008; 77(4): 791 - 799.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. B. Yu, D. Y. Deng, H. Beppu, C. C. Hong, C. Lai, S. A. Hoyng, N. Kawai, and K. D. Bloch
Bone Morphogenetic Protein (BMP) Type II Receptor Is Required for BMP-mediated Growth Arrest and Differentiation in Pulmonary Artery Smooth Muscle Cells
J. Biol. Chem., February 15, 2008; 283(7): 3877 - 3888.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
M. M. C. Kong, A. Hasbi, M. Mattocks, T. Fan, B. F. O'Dowd, and S. R. George
Regulation of D1 Dopamine Receptor Trafficking and Signaling by Caveolin-1
Mol. Pharmacol., November 1, 2007; 72(5): 1157 - 1170.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Shmuel, E. Nodel-Berner, T. Hyman, A. Rouvinski, and Y. Altschuler
Caveolin 2 Regulates Endocytosis and Trafficking of the M1 Muscarinic Receptor in MDCK Epithelial Cells
Mol. Biol. Cell, May 1, 2007; 18(5): 1570 - 1585.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
P.-K. Fang, K. R. Solomon, L. Zhuang, M. Qi, M. McKee, M. R. Freeman, and P. C. Yelick
Caveolin-1{alpha} and -1{beta} Perform Nonredundant Roles in Early Vertebrate Development
Am. J. Pathol., December 1, 2006; 169(6): 2209 - 2222.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Hartung, K. Bitton-Worms, M. M. Rechtman, V. Wenzel, J. H. Boergermann, S. Hassel, Y. I. Henis, and P. Knaus
Different Routes of Bone Morphogenic Protein (BMP) Receptor Endocytosis Influence BMP Signaling
Mol. Cell. Biol., October 15, 2006; 26(20): 7791 - 7805.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005