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JCS ePress
online publication date 12 Oct 2004
doi: 10.1242/jcs.01416
Research Article
Spatial mapping of integrin interactions and dynamics during cell migration by Image Correlation Microscopy
Paul W. Wiseman,
Claire M. Brown*,
Donna J. Webb,
Benedict Hebert,
Natalie L. Johnson,
Jeff A. Squier,
Mark H. Ellisman,
and
A. F. Horwitz
* Author for correspondence (e-mail: cmb8t{at}virginia.edu)
Image correlation microscopy methodology was extended and used to determine retrospectively the density, dynamics and interactions of 
5-integrin in migrating cells. 5-integrin is present in submicroscopic clusters containing 3-4 integrins before it is discernibly organized. The integrin in nascent adhesions, as identified by the presence of paxillin, is 
1.4 times more concentrated, 4.5 times more clustered and much less mobile than in surrounding regions. Thus, while integrins are clustered throughout the cell, they differ in nascent adhesions and appear to initiate adhesion formation, despite their lack of visible organization. In more mature adhesions where the integrin is visibly organized there are 900 integrins µm-2 (about fivefold higher than surrounding regions). Interestingly, 5-integrin and -actinin, but not paxillin, reside in a complex throughout the cell, where they diffuse and flow together, even in regions where they are not organized. During adhesion disassembly some integrins diffuse away slowly, -actinin undergoes a directed movement at speeds similar to actin retrograde flow (0.29 µm min-1), while all of the paxillin diffuses away rapidly.
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© The Company of Biologists Ltd 2004
