spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 7 Dec 2004
doi: 10.1242/jcs.01570

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01570v1
118/1/39    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dunaway, S.
Right arrow Articles by Walworth, N. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dunaway, S.
Right arrow Articles by Walworth, N. C.

Research Article

Interaction of 14-3-3 protein with Chk1 affects localization and checkpoint function


Stephen Dunaway, Hsing-Yin Liu, and Nancy C. Walworth*
* Author for correspondence (e-mail: walworna{at}umdnj.edu)

The protein kinase Chk1 is required for proper arrest of the cell cycle in response to DNA damage. We have previously shown in Schizosaccharomyces pombe, that upon DNA damage, phosphorylation of Chk1 correlates with checkpoint activation and that phosphorylated Chk1 is capable of interacting with the 14-3-3 proteins, Rad24 and Rad25. The interaction between Rad24 and Chk1 is stimulated tenfold after exposure to DNA damaging agents and we postulate that it is an important event in the DNA damage checkpoint response pathway in fission yeast. We identified a stretch of leucine residues as the domain in Chk1 that mediates the interaction with 14-3-3 proteins. Substitution of leucine residues with alanine disrupts the interaction with Rad24 and also prevents Chk1 from becoming phosphorylated in response to DNA damaging agents. Cells expressing the mutants are sensitive to UV radiation. In this study, we also show that Chk1 accumulates in the nucleus in response to DNA damage and this behavior is dependent on Rad24. Interestingly, the 14-3-3 binding domain mutants also fail to localize to the nucleus prompting a search for localization sequences within Chk1. Our investigations have identified the presence of both functional nuclear import and nuclear export sequences encoded in S. pombe Chk1 that, in conjunction with 14-3-3 proteins, may play a prominent role in regulating Chk1 localization and function.


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
A. Kosoy and M. J. O'Connell
Regulation of Chk1 by Its C-terminal Domain
Mol. Biol. Cell, November 1, 2008; 19(11): 4546 - 4553.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. Diaz-Cuervo and A. Bueno
Cds1 Controls the Release of Cdc14-like Phosphatase Flp1 from the Nucleolus to Drive Full Activation of the Checkpoint Response to Replication Stress in Fission Yeast
Mol. Biol. Cell, June 1, 2008; 19(6): 2488 - 2499.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Niida, Y. Katsuno, B. Banerjee, M. P. Hande, and M. Nakanishi
Specific Role of Chk1 Phosphorylations in Cell Survival and Checkpoint Activation
Mol. Cell. Biol., April 1, 2007; 27(7): 2572 - 2581.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Usui and J. H. J. Petrini
The Saccharomyces cerevisiae 14-3-3 proteins Bmh1 and Bmh2 directly influence the DNA damage-dependent functions of Rad53
PNAS, February 20, 2007; 104(8): 2797 - 2802.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Pereg, S. Lam, A. Teunisse, S. Biton, E. Meulmeester, L. Mittelman, G. Buscemi, K. Okamoto, Y. Taya, Y. Shiloh, et al.
Differential Roles of ATM- and Chk2-Mediated Phosphorylations of Hdmx in Response to DNA Damage.
Mol. Cell. Biol., September 1, 2006; 26(18): 6819 - 6831.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004