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JCS ePress online publication date 25 Jan 2005
doi: 10.1242/jcs.01648


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Research Article

Integrin-dependent interaction of lipid rafts with the actin cytoskeleton in activated human platelets


Stéphane Bodin, Carine Soulet, Hélène Tronchère, Pierre Sié, Christian Gachet, Monique Plantavid, and Bernard Payrastre*
* Author for correspondence (e-mail: payrastr{at}toulouse.inserm.fr)

Dynamic connections between actin filaments and the plasma membrane are crucial for the regulation of blood platelet functions. Protein complexes associated with {alpha}IIb{beta}3 integrin-based cytoskeleton structures are known to play a role in these processes. However, mechanisms involving lateral organizations of the plasma membrane remain to be investigated. Here, we demonstrate that a large fraction of platelet lipid rafts specifically associates with the actin cytoskeleton upon activation. This association was inhibited by antagonists of fibrinogen-{alpha}IIb{beta}3 binding and did not occur in type I Glanzman's thrombasthenic platelets. The raft-cytoskeleton interaction is a reversible process correlating with the intensity and stability of platelet aggregation. Although only a minor fraction of {alpha}IIb{beta}3 was recovered in rafts upon activation, this integrin specifically upregulated the level of PtdIns(4,5)P2 in membrane microdomains and induced the recruitment of several actin-modulating proteins known to directly or indirectly interact with this lipid. Controlled disruption of rafts did not affect {alpha}IIb{beta}3-mediated platelet aggregation in response to high concentrations of thrombin but significantly inhibited fibrin clot retraction. We propose that rafts participate in the organization of membrane-cytoskeleton interactions where {alpha}IIb{beta}3-mediated tension forces apply during the late phase of platelet activation.


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