spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 25 Jan 2005
doi: 10.1242/jcs.01663

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01663v1
118/4/733    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pandey, R.
Right arrow Articles by Lehner, C. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pandey, R.
Right arrow Articles by Lehner, C. F.

Research Article

Epithelial re-organization and dynamics of progression through mitosis in Drosophila separase complex mutants


Rahul Pandey, Stefan Heidmann, and Christian F. Lehner*
* Author for correspondence (e-mail: chle{at}uni-bayreuth.de)

Separase cleaves a subunit of the cohesin complex and thereby promotes sister chromatid separation during mitotic and meiotic divisions. Drosophila separase associates with regulatory subunits encoded by the pimples and three rows genes. Three rows and Pimples, the Drosophila securin, are required for sister chromatid separation during mitosis. Budding yeast separase provides other functions in addition to cohesin subunit cleavage, which are required for spindle organization and temporal regulation during exit from mitosis. Therefore, using time-lapse imaging in live embryos, we have carefully analyzed progression through mitosis in pimples and three rows mutants. We demonstrate that despite the total failure of sister chromatid separation, exit from mitosis, including a complete cytokinesis, proceeds with only a minor temporal delay in the epidermal cells of these mutants. Interestingly, however, pronounced defects in the epithelial organization develop in the following interphase, indicating that the separase complex is not only important for genetic stability but also and perhaps indirectly for epithelial integrity.


This article has been cited by other articles:


Home page
 Genes Dev.Home page
H. Wen, L. Andrejka, J. Ashton, R. Karess, and J. S. Lipsick
Epigenetic regulation of gene expression by Drosophila Myb and E2F2-RBF via the Myb-MuvB/dREAM complex
Genes & Dev., March 1, 2008; 22(5): 601 - 614.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. L. Shepard, J. F. Amatruda, D. Finkelstein, J. Ziai, K. R. Finley, H. M. Stern, K. Chiang, C. Hersey, B. Barut, J. L. Freeman, et al.
A mutation in separase causes genome instability and increased susceptibility to epithelial cancer
Genes & Dev., January 1, 2007; 21(1): 55 - 59.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Z. Liu and C. A. Makaroff
Arabidopsis Separase AESP Is Essential for Embryo Development and the Release of Cohesin during Meiosis
PLANT CELL, May 1, 2006; 18(5): 1213 - 1225.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
K. G. Wirth, G. Wutz, N. R. Kudo, C. Desdouets, A. Zetterberg, S. Taghybeeglu, J. Seznec, G. M. Ducos, R. Ricci, N. Firnberg, et al.
Separase: a universal trigger for sister chromatid disjunction but not chromosome cycle progression.
J. Cell Biol., March 13, 2006; 172(6): 847 - 860.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. Heeger, O. Leismann, R. Schittenhelm, O. Schraidt, S. Heidmann, and C. F. Lehner
Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog
Genes & Dev., September 1, 2005; 19(17): 2041 - 2053.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005