spacer gif spacer gif spacer gif spacer gif spacer gif
 QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 11 Mar 2008
doi: 10.1242/jcs.019455


This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.019455v1
121/7/1036    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alcorn, J. F.
Right arrow Articles by Janssen-Heininger, Y. M.W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alcorn, J. F.
Right arrow Articles by Janssen-Heininger, Y. M.W.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Jun N-terminal kinase 1 regulates epithelial-to-mesenchymal transition induced by TGF-{beta}1


John F. Alcorn, Amy S. Guala, Jos van der Velden, Brian McElhinney, Charles G. Irvin, Roger J. Davis, and Yvonne M.W. Janssen-Heininger*
* Author for correspondence (e-mail: yvonne.janssen{at}uvm.edu)

Transforming growth factor {beta}1 (TGF-{beta}1) is a cardinal cytokine in the pathogenesis of airway remodeling, and promotes epithelial-to-mesenchymal transition (EMT). As a molecular interaction between TGF-{beta}1 and Jun N-terminal kinase (JNK) has been demonstrated, the goal of this study was to elucidate whether JNK plays a role in TGF-{beta}1-induced EMT. Primary cultures of mouse tracheal epithelial cells (MTEC) from wild-type, JNK1-/- or JNK2-/- mice were comparatively evaluated for their ability to undergo EMT in response to TGF-{beta}1. Wild-type MTEC exposed to TGF-{beta}1 demonstrated a prominent induction of mesenchymal mediators and a loss of epithelial markers, in conjunction with a loss of trans-epithelial resistance (TER). Significantly, TGF-{beta}1-mediated EMT was markedly blunted in epithelial cells lacking JNK1, while JNK2-/- MTEC underwent EMT in response to TGF-{beta}1 in a similar way to wild-type cells. Although Smad2/3 phosphorylation and nuclear localization of Smad4 were similar in JNK1-/- MTEC in response to TGF-{beta}1, Smad DNA-binding activity was diminished. Gene expression profiling demonstrated a global suppression of TGF-{beta}1-modulated genes, including regulators of EMT in JNK1-/- MTEC, in comparison with wild-type cells. In aggregate, these results illuminate the novel role of airway epithelial-dependent JNK1 activation in EMT.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
Mol. Cell. Biol.Home page
A. N. Abell, D. A. Granger, N. L. Johnson, N. Vincent-Jordan, C. F. Dibble, and G. L. Johnson
Trophoblast Stem Cell Maintenance by Fibroblast Growth Factor 4 Requires MEKK4 Activation of Jun N-Terminal Kinase
Mol. Cell. Biol., May 15, 2009; 29(10): 2748 - 2761.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Cell Mol. Bio.Home page
S. Matalon and K. B. Adler
Highlights of the April Issue
Am. J. Respir. Cell Mol. Biol., April 1, 2009; 40(4): 383 - 384.
[Full Text] [PDF]


Home page
Am. J. Respir. Cell Mol. Bio.Home page
J. F. Alcorn, J. van der Velden, A. L. Brown, B. McElhinney, C. G. Irvin, and Y. M. W. Janssen-Heininger
c-Jun N-Terminal Kinase 1 Is Required for the Development of Pulmonary Fibrosis
Am. J. Respir. Cell Mol. Biol., April 1, 2009; 40(4): 422 - 432.
[Abstract] [Full Text] [PDF]


Home page
Mol Cancer ResHome page
N. Garamszegi, S. P. Garamszegi, L. A. Shehadeh, and S. P. Scully
Extracellular Matrix-Induced Gene Expression in Human Breast Cancer Cells
Mol. Cancer Res., March 1, 2009; 7(3): 319 - 329.
[Abstract] [Full Text] [PDF]




© The Company of Biologists Ltd 2008