The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 31 May 2005
doi: 10.1242/jcs.02410
Research Article
Klp67A destabilises pre-anaphase microtubules but subsequently is required to stabilise the central spindle
Melanie K. Gatt,
Matthew S. Savoian*,
Maria G. Riparbelli,
Chiara Massarelli,
Giuliano Callaini,
and
David M. Glover
* Author for correspondence (e-mail: ms476{at}mole.bio.cam.ac.uk)
Klp67A is a member of the Kip3 subfamily of microtubule destabilising kinesins, the loss of which results in abnormally long and stable pre-anaphase microtubules. Here we examine its role during cytokinesis in Drosophila primary spermatocytes that require the coordinated interaction of an interior and peripheral set of central spindle microtubules. In mutants anaphase B spindles elongated with normal kinetics but bent towards the cortex. Both peripheral and interior spindle microtubules then formed diminished bundles of abnormally positioned central spindle microtubules associated with the pavarotti-KLP and KLP3A motor proteins. The minus ends of these were poorly aligned as revealed by Asp protein localisation. Furrows always initiated at the sites of central spindle bundles but could be unilateral or nonequatorially positioned. Ectopic furrows were stimulated by the interior central spindle and formed only after this structure buckled and contacted the cortex. Furrows often halted and regressed as they could not be sustained by the central spindles that became increasing unstable over time and often completely degraded. Consistent with this, actin and anillin failed to form homogenous bands. Thus, the Klp67A microtubule catastrophe factor is required for cytokinesis by regulating both the formation and stability of the central spindle.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
P. M. Grissom, T. Fiedler, E. L. Grishchuk, D. Nicastro, R. R. West, and J. Richard McIntosh
Kinesin-8 from Fission Yeast: A Heterodimeric, Plus-End-directed Motor that Can Couple Microtubule Depolymerization to Cargo Movement
Mol. Biol. Cell,
February 1, 2009;
20(3):
963 - 972.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. W. Buster, D. Zhang, and D. J. Sharp
Poleward Tubulin Flux in Spindles: Regulation and Function in Mitotic Cells
Mol. Biol. Cell,
August 1, 2007;
18(8):
3094 - 3104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. P. D'Avino, M. S. Savoian, L. Capalbo, and D. M. Glover
RacGAP50C is sufficient to signal cleavage furrow formation during cytokinesis
J. Cell Sci.,
November 1, 2006;
119(21):
4402 - 4408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Grallert, C. Beuter, R. A. Craven, S. Bagley, D. Wilks, U. Fleig, and I. M. Hagan
S. pombe CLASP needs dynein, not EB1 or CLIP170, to induce microtubule instability and slows polymerization rates at cell tips in a dynein-dependent manner
Genes & Dev.,
September 1, 2006;
20(17):
2421 - 2436.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Gatt and D. M. Glover
The Drosophila phosphatidylinositol transfer protein encoded by vibrator is essential to maintain cleavage-furrow ingression in cytokinesis
J. Cell Sci.,
June 1, 2006;
119(11):
2225 - 2235.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Zavortink, N. Contreras, T. Addy, A. Bejsovec, and R. Saint
Tum/RacGAP50C provides a critical link between anaphase microtubules and the assembly of the contractile ring in Drosophila melanogaster
J. Cell Sci.,
November 15, 2005;
118(22):
5381 - 5392.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2005
