spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 19 Jul 2005
doi: 10.1242/jcs.02453

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02453v1
118/15/3289    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Furuya, K.
Right arrow Articles by Furuya, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Furuya, K.
Right arrow Articles by Furuya, S.

Research Article

Characteristics of subepithelial fibroblasts as a mechano-sensor in the intestine: cell-shape-dependent ATP release and P2Y1 signaling


Kishio Furuya*, Masahiro Sokabe, and Sonoko Furuya
* Author for correspondence (e-mail: furuya{at}med.nagoya-u.ac.jp)

Subepithelial fibroblasts form a cellular network just under the epithelium of the gastrointestinal tract. Using primary cultured cells isolated from rat duodenal villi, we previously found that subepithelial fibroblasts reversibly changed cell morphology between flat and stellate-shape depending on intracellular cAMP levels. In this paper, we examined cell-cell communication via released ATP and Ca2+ signaling in the cellular network. Subepithelial fibroblasts were sensitive to mechanical stress such as 'touching' a cell with a fine glass rod and 'stretching' cells cultured on elastic silicone chamber. Mechanical stimulations evoked Ca2+-increase in the cells and ATP-release from the cells. The released ATP activated P2Y receptors on the surrounding cells and propagated Ca2+-waves through the network. Concomitant with Ca2+-waves, a transient contraction of the network was observed. Histochemical, RT-PCR, western blotting and Ca2+ response analyses indicated P2Y1 is a dominant functional subtype. ATP-release and Ca2+ signaling were cell-shape dependent, i.e. they were abolished in stellate-shaped cells treated with dBcAMP, and recovered or further enhanced in re-flattened cells treated with endothelin. The response to ATP also decreased in stellate-shaped cells. These findings indicate cAMP-mediated intracellular signaling causes cell-shape change, which accompanies the changes in mechano- and ATP sensitivities. Using a co-culture system of neuronal cells (NG108-15) with subepithelial fibroblasts, we confirmed that mechanically induced Ca2+-waves propagated to neurons. From these findings we propose that subepithelial fibroblasts work as a mechano-sensor in the intestine. Uptake of food, water and nutrients may cause mechanical stress on subepithelial fibroblasts in the villi. The ATP released by mechanical stimulation elicits Ca2+-wave propagation through the network via P2Y1 activation and also activates P2X on terminals of mucosal sensory neurons to regulate peristaltic motility.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Kawai, M. Kaidoh, and T. Ohhashi
MDA-MB-231 produces ATP-mediated ICAM-1-dependent facilitation of the attachment of carcinoma cells to human lymphatic endothelial cells
Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1123 - C1132.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. Follonier, S. Schaub, J.-J. Meister, and B. Hinz
Myofibroblast communication is controlled by intercellular mechanical coupling
J. Cell Sci., October 15, 2008; 121(20): 3305 - 3316.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
S. Wongkhantee, T. Yongchaitrakul, and P. Pavasant
Mechanical Stress Induces Osteopontin via ATP/P2Y1 in Periodontal Cells
J. Dent. Res., June 1, 2008; 87(6): 564 - 568.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. Chopra, J. Gever, S. R. Barrick, A. T. Hanna-Mitchell, J. M. Beckel, A. P. D. W. Ford, and L. A. Birder
Expression and function of rat urothelial P2Y receptors
Am J Physiol Renal Physiol, April 1, 2008; 294(4): F821 - F829.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. Yamamoto, N. Shimizu, S. Obi, S. Kumagaya, Y. Taketani, A. Kamiya, and J. Ando
Involvement of cell surface ATP synthase in flow-induced ATP release by vascular endothelial cells
Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1646 - H1653.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. M. Gwynne and J. C. Bornstein
Local inhibitory reflexes excited by mucosal application of nutrient amino acids in guinea pig jejunum
Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1660 - G1670.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. L. Winters, C. W. Davis, and R. C. Boucher
Mechanosensitivity of mouse tracheal ciliary beat frequency: roles for Ca2+, purinergic signaling, tonicity, and viscosity
Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L614 - L624.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005