spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 2 Aug 2005
doi: 10.1242/jcs.02514

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02514v1
118/16/3805    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lambotin, M.
Right arrow Articles by Bourdoulous, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lambotin, M.
Right arrow Articles by Bourdoulous, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Invasion of endothelial cells by Neisseria meningitidis requires cortactin recruitment by a phosphoinositide-3-kinase/Rac1 signalling pathway triggered by the lipo-oligosaccharide


Mélanie Lambotin, Isabelle Hoffmann, Marie-Pierre Laran-Chich, Xavier Nassif, Pierre Olivier Couraud, and Sandrine Bourdoulous*
* Author for correspondence (e-mail: bourdoulous{at}cochin.inserm.fr)

Type-IV-pilus-mediated adhesion of Neisseria meningitidis (also known as meningococcus) to human endothelial cells induces the formation of membrane protrusions leading to bacterial uptake. We have previously shown that these protrusions result from a Rho- and Cdc42-dependent cortical actin polymerization, and from the activation of the ErbB2 tyrosine-kinase receptor and the Src kinase, leading to tyrosine phosphorylation of cortactin. We report here that N. meningitidis mutants expressing a deglycosylated lipo-oligosaccharide are poorly invasive. These mutants show structurally altered actin polymerization. Moreover, although they efficiently recruit and activate ErbB2 and Src, these mutants are defective in the recruitment and phosphorylation of cortactin. We demonstrate that phosphorylated cortactin controls the cortical actin polymerization, which leads to membrane protrusion formation. In addition, we show that cortactin recruitment is dependent on the activation of a phosphoinositide-3-kinase/Rac1-GTPase signalling pathway, which is required for actin polymerization and internalization of N. meningitidis, and is not activated by the mutant strains. Altogether, these results define a new role for the lipo-oligosaccharide in triggering a phosphoinositide-3-kinase/Rac1 signalling required to elicit an efficient uptake of N. meningitidis in non-phagocytic cells.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Nerlich, M. Rohde, S. R. Talay, H. Genth, I. Just, and G. S. Chhatwal
Invasion of Endothelial Cells by Tissue-invasive M3 Type Group A Streptococci Requires Src Kinase and Activation of Rac1 by a Phosphatidylinositol 3-Kinase-independent Mechanism
J. Biol. Chem., July 24, 2009; 284(30): 20319 - 20328.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. Louwen, A. Heikema, A. van Belkum, A. Ott, M. Gilbert, W. Ang, H. P. Endtz, M. P. Bergman, and E. E. Nieuwenhuis
The Sialylated Lipooligosaccharide Outer Core in Campylobacter jejuni Is an Important Determinant for Epithelial Cell Invasion
Infect. Immun., October 1, 2008; 76(10): 4431 - 4438.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Tehrani, R. Faccio, I. Chandrasekar, F. P. Ross, and J. A. Cooper
Cortactin Has an Essential and Specific Role in Osteoclast Actin Assembly
Mol. Biol. Cell, July 1, 2006; 17(7): 2882 - 2895.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
N. Doulet, E. Donnadieu, M.-P. Laran-Chich, F. Niedergang, X. Nassif, P. O. Couraud, and S. Bourdoulous
Neisseria meningitidis infection of human endothelial cells interferes with leukocyte transmigration by preventing the formation of endothelial docking structures
J. Cell Biol., May 22, 2006; 173(4): 627 - 637.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005