spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 23 Aug 2005
doi: 10.1242/jcs.02531

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02531v1
118/18/4113    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Salaycik, K. J.
Right arrow Articles by Wadsworth, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salaycik, K. J.
Right arrow Articles by Wadsworth, P.

Research Article

Quantification of microtubule nucleation, growth and dynamics in wound-edge cells


Kimberly J. Salaycik, Carey J. Fagerstrom, Kausalya Murthy, U. Serdar Tulu, and Patricia Wadsworth*
* Author for correspondence (e-mail: patw{at}bio.umass.edu)

Mammalian cells develop a polarized morphology and migrate directionally into a wound in a monolayer culture. To understand how microtubules contribute to these processes, we used GFP-tubulin to measure dynamic instability and GFP-EB1, a protein that marks microtubule plus-ends, to measure microtubule growth events at the centrosome and cell periphery. Growth events at the centrosome, or nucleation, do not show directional bias, but are equivalent toward and away from the wound. Cells with two centrosomes nucleated approximately twice as many microtubules/minute as cells with one centrosome. The average number of growing microtubules per µm2 at the cell periphery is similar for leading and trailing edges and for cells containing one or two centrosomes. In contrast to microtubule growth, measurement of the parameters of microtubule dynamic instability demonstrate that microtubules in the trailing edge are more dynamic than those in the leading edge. Inhibition of Rho with C3 transferase had no detectable effect on microtubule dynamics in the leading edge, but stimulated microtubule turnover in the trailing edge. Our data demonstrate that in wound-edge cells, microtubule nucleation is non-polarized, in contrast to microtubule dynamic instability, which is highly polarized, and that factors in addition to Rho contribute to microtubule stabilization.


This article has been cited by other articles:


Home page
 Physiol. GenomicsHome page
S. Roy, S. Khanna, C. Rink, S. Biswas, and C. K. Sen
Characterization of the acute temporal changes in excisional murine cutaneous wound inflammation by screening of the wound-edge transcriptome
Physiol Genomics, July 9, 2008; 34(2): 162 - 184.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. D.-A. Tran, T. P. Marmo, A. A. Salam, S. Che, E. Finkelstein, R. Kabarriti, H. S. Xenias, R. Mazitschek, C. Hubbert, Y. Kawaguchi, et al.
HDAC6 deacetylation of tubulin modulates dynamics of cellular adhesions
J. Cell Sci., April 15, 2007; 120(8): 1469 - 1479.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
K. Onishi, M. Higuchi, T. Asakura, N. Masuyama, and Y. Gotoh
The PI3K-Akt pathway promotes microtubule stabilization in migrating fibroblasts
Genes Cells, April 1, 2007; 12(4): 535 - 546.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
B. Schermer, C. Ghenoiu, M. Bartram, R. U. Muller, F. Kotsis, M. Hohne, W. Kuhn, M. Rapka, R. Nitschke, H. Zentgraf, et al.
The von Hippel-Lindau tumor suppressor protein controls ciliogenesis by orienting microtubule growth
J. Cell Biol., November 20, 2006; 175(4): 547 - 554.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Togo
Disruption of the plasma membrane stimulates rearrangement of microtubules and lipid traffic toward the wound site
J. Cell Sci., July 1, 2006; 119(13): 2780 - 2786.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005