spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 18 Oct 2005
doi: 10.1242/jcs.02618

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02618v1
118/21/5089    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krejci, P.
Right arrow Articles by Wilcox, W. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krejci, P.
Right arrow Articles by Wilcox, W. R.

Research Article

Interaction of fibroblast growth factor and C-natriuretic peptide signaling in regulation of chondrocyte proliferation and extracellular matrix homeostasis


Pavel Krejci, Bernard Masri, Vincent Fontaine, Pertchoui B. Mekikian, MaryAnn Weis, Herve Prats, and William R. Wilcox*
* Author for correspondence (e-mail: william.wilcox{at}cshs.org)

Overexpression of C-natriuretic peptide (CNP) in cartilage partially rescues achondroplasia in the mouse. Here, we studied the interaction of fibroblast growth factor (FGF) and CNP signaling in chondrocytes. CNP antagonized FGF2-induced growth arrest of rat chondrosarcoma (RCS) chondrocytes by inhibition of the Erk mitogen activated protein kinase pathway. This effect of CNP was protein kinase G-dependent and was mimicked by the cGMP analog pCPT-cGMP. FGF2-mediated activation of both MEK and Raf-1 but not Ras or FRS2 was abolished by CNP demonstrating that CNP blocks the Erk pathway at the level of Raf-1. CNP also counteracted the FGF2-mediated degradation of RCS extracellular matrix. CNP partially antagonized FGF2-induced expression, release and activation of several matrix-remodeling molecules including matrix metalloproteinase 2 (MMP2), MMP3, MMP9, MMP10 and MMP13. In addition, CNP compensated for FGF2-mediated matrix loss by upregulation of matrix production independent of its interference with FGF signaling. We conclude that CNP utilizes both direct and indirect ways to counteract the effects of FGF signaling in a chondrocyte environment.


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
P. Krejci, L. Salazar, H. S. Goodridge, T. A. Kashiwada, M. J. Schibler, P. Jelinkova, L. M. Thompson, and W. R. Wilcox
STAT1 and STAT3 do not participate in FGF-mediated growth arrest in chondrocytes
J. Cell Sci., February 1, 2008; 121(3): 272 - 281.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Moffatt, G. Thomas, K. Sellin, M.-C. Bessette, F. Lafreniere, O. Akhouayri, R. St-Arnaud, and C. Lanctot
Osteocrin Is a Specific Ligand of the Natriuretic Peptide Clearance Receptor That Modulates Bone Growth
J. Biol. Chem., December 14, 2007; 282(50): 36454 - 36462.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Krejci, B. Masri, L. Salazar, C. Farrington-Rock, H. Prats, L. M. Thompson, and W. R. Wilcox
Bisindolylmaleimide I Suppresses Fibroblast Growth Factor-mediated Activation of Erk MAP Kinase in Chondrocytes by Preventing Shp2 Association with the Frs2 and Gab1 Adaptor Proteins
J. Biol. Chem., February 2, 2007; 282(5): 2929 - 2936.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Karakiulakis, E. Papakonstantinou, A. J. Aletras, M. Tamm, and M. Roth
Cell Type-specific Effect of Hypoxia and Platelet-derived Growth Factor-BB on Extracellular Matrix Turnover and Its Consequences for Lung Remodeling
J. Biol. Chem., January 12, 2007; 282(2): 908 - 915.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005