spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 14 Feb 2006
doi: 10.1242/jcs.02785

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02785v1
119/5/807    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Anitei, M.
Right arrow Articles by Pfeiffer, S. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Anitei, M.
Right arrow Articles by Pfeiffer, S. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

A role for Sec8 in oligodendrocyte morphological differentiation


Mihaela Anitei, Marius Ifrim, Marie-Ann Ewart, Ann E. Cowan, John H. Carson, Rashmi Bansal, and Steven E. Pfeiffer*
* Author for correspondence (e-mail: pfeiffer{at}neuron.uchc.edu)

In the central nervous system, oligodendrocytes synthesize vast amounts of myelin, a multilamellar membrane wrapped around axons that dramatically enhances nerve transmission. A complex apparatus appears to coordinate trafficking of proteins and lipids during myelin synthesis, but the molecular interactions involved are not well understood. We demonstrate that oligodendrocytes express several key molecules necessary for the targeting of transport vesicles to areas of rapid membrane growth, including the exocyst components Sec8 and Sec6 and the multidomain scaffolding proteins CASK and Mint1. Sec8 overexpression significantly promotes oligodendrocyte morphological differentiation and myelin-like membrane formation in vitro; conversely, siRNA-mediated interference with Sec8 expression inhibits this process, and anti-Sec8 antibody induces a reduction in oligodendrocyte areas. In addition, Sec8 colocalizes, coimmunoprecipitates and cofractionates with the major myelin protein OSP/Claudin11 and with CASK in oligodendrocytes. These results suggest that Sec8 plays a central role in oligodendrocyte membrane formation by regulating the recruitment of vesicles that transport myelin proteins such as OSP/Claudin11 to sites of membrane growth.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
A. Bolis, S. Coviello, I. Visigalli, C. Taveggia, A. Bachi, A. H. Chishti, T. Hanada, A. Quattrini, S. C. Previtali, A. Biffi, et al.
Dlg1, Sec8, and Mtmr2 Regulate Membrane Homeostasis in Schwann Cell Myelination
J. Neurosci., July 8, 2009; 29(27): 8858 - 8870.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
G. Lalli
RalA and the exocyst complex influence neuronal polarity through PAR-3 and aPKC
J. Cell Sci., May 15, 2009; 122(10): 1499 - 1506.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. S. Spiczka and C. Yeaman
Ral-regulated interaction between Sec5 and paxillin targets Exocyst to focal complexes during cell migration
J. Cell Sci., September 1, 2008; 121(17): 2880 - 2891.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. Winterstein, J. Trotter, and E.-M. Kramer-Albers
Distinct endocytic recycling of myelin proteins promotes oligodendroglial membrane remodeling
J. Cell Sci., March 15, 2008; 121(6): 834 - 842.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Simons and K. Trajkovic
Neuron-glia communication in the control of oligodendrocyte function and myelin biogenesis
J. Cell Sci., November 1, 2006; 119(21): 4381 - 4389.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006