spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 28 Mar 2006
doi: 10.1242/jcs.02845

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02845v1
119/8/1666    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, Y.
Right arrow Articles by He, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, Y.
Right arrow Articles by He, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Interaction of SH2-B{beta} with RET is involved in signaling of GDNF-induced neurite outgrowth


Yong Zhang, Wei Zhu, Yong-Gang Wang, Xiu-Jie Liu, Li Jiao, Xuan Liu, Zhao-Huan Zhang, Chang-Lin Lu, and Cheng He*
* Author for correspondence (e-mail: chenghe{at}online.sh.cn)

RET receptor signalling is essential for glial-cell-line-derived neurotrophic factor (GDNF)-induced survival and differentiation of various neurons such as mesencephalic neurons. To identify proteins that mediate RET-dependent signaling, yeast two-hybrid screening was performed with the intracellular domain of RET as bait. We identified a new interaction between RET and the adapter protein SH2-B{beta}. Upon GDNF stimulation of PC12-GFR{alpha}1-RET cells (that stably overexpress GDNF receptor {alpha}1 and RET), wild-type SH2-B{beta} co-immunoprecipitated with RET, whereas the dominant-negative SH2-B{beta} mutant R555E did not. RET interacted with endogenous SH2-B{beta} both in PC12-GFR{alpha}1-RET cells and in rat tissues. Mutagenesis analysis revealed that Tyr981 within the intracellular domain of RET was crucial for the interaction with SH2-B{beta}. Morphological evidence showed that SH2-B{beta} and RET colocalized in mesencephalic neurons. Furthermore, functional analysis indicated that overexpression of SH2-B{beta} facilitated GDNF-induced neurite outgrowth in both PC12-GFR{alpha}1-RET cells and cultured mesencephalic neurons, whereas the mutant R555E inhibited the effect. Moreover, inhibition of SH2-B{beta} expression by RNA interference caused a significant decrease of GDNF-induced neuronal differentiation in PC12-GFR{alpha}1-RET cells. Taken together, our results suggest that SH2-B{beta} is a new signaling molecule involved in GDNF-induced neurite outgrowth.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
T. J. Maures, L. Chen, and C. Carter-Su
Nucleocytoplasmic Shuttling of the Adapter Protein SH2B1{beta} (SH2-B{beta}) Is Required for Nerve Growth Factor (NGF)-Dependent Neurite Outgrowth and Enhancement of Expression of a Subset of NGF-Responsive Genes
Mol. Endocrinol., July 1, 2009; 23(7): 1077 - 1091.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Zhang, X. Xu, Y. Zhang, J. Zhou, Z. Yu, and C. He
LINGO-1 Interacts with WNK1 to Regulate Nogo-induced Inhibition of Neurite Extension
J. Biol. Chem., June 5, 2009; 284(23): 15717 - 15728.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhang, Y.-G. Wang, Q. Zhang, X.-J. Liu, X. Liu, L. Jiao, W. Zhu, Z.-H. Zhang, X.-L. Zhao, and C. He
Interaction of Mint2 with TrkA Is Involved in Regulation of Nerve Growth Factor-induced Neurite Outgrowth
J. Biol. Chem., May 1, 2009; 284(18): 12469 - 12479.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
L. Chen, T. J. Maures, H. Jin, J. S. Huo, S. A. Rabbani, J. Schwartz, and C. Carter-Su
SH2B1{beta} (SH2-B{beta}) Enhances Expression of a Subset of Nerve Growth Factor-Regulated Genes Important for Neuronal Differentiation Including Genes Encoding Urokinase Plasminogen Activator Receptor and Matrix Metalloproteinase 3/10
Mol. Endocrinol., February 1, 2008; 22(2): 454 - 476.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006