spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 28 Mar 2006
doi: 10.1242/jcs.02875

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02875v1
119/8/1604    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tothova, J.
Right arrow Articles by Schiaffino, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tothova, J.
Right arrow Articles by Schiaffino, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

NFATc1 nucleocytoplasmic shuttling is controlled by nerve activity in skeletal muscle


Jana Tothova, Bert Blaauw, Giorgia Pallafacchina, Rüdiger Rudolf, Carla Argentini, Carlo Reggiani, and Stefano Schiaffino*
* Author for correspondence (e-mail: stefano.schiaffino{at}unipd.it)

Calcineurin-NFAT signaling has been shown to control activity-dependent muscle gene regulation and induce a program of gene expression typical of slow oxidative muscle fibers. Following Ca2+-calmodulin stimulation, calcineurin dephosphorylates NFAT proteins and induces their translocation into the nucleus. However, NFAT nuclear translocation has never been investigated in skeletal muscle in vivo. To determine whether NFATc1 nucleocytoplasmic shuttling depends on muscle activity, we transfected fast and slow mouse muscles with plasmids coding for an NFATc1-GFP fusion protein. We found that NFATc1-GFP has a predominantly cytoplasmic localization in the fast tibialis anterior muscle but a predominantly nuclear localization in the slow soleus muscle, with a characteristic focal intranuclear distribution. Two hours of complete inactivity, induced by denervation or anaesthesia, cause NFATc1 export out of the nucleus in soleus muscle fibers, whereas electrostimulation of tibialis anterior with a low-frequency tonic impulse pattern, mimicking the firing pattern of slow motor neurons, causes NFATc1 nuclear translocation. The activity-dependent nuclear import and export of NFATc1 is a rapid event, as visualized directly in vivo by two-photon microscopy. The calcineurin inhibitor cain/cabin1 causes nuclear export of NFATc1 both in normal soleus and stimulated tibialis anterior muscle. These findings support the notion that in skeletal muscle NFATc1 is a calcineurin-dependent nerve activity sensor.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Physiol.Home page
M. Murgia, T. E. Jensen, M. Cusinato, M. Garcia, E. A. Richter, and S. Schiaffino
Multiple signalling pathways redundantly control glucose transporter GLUT4 gene transcription in skeletal muscle
J. Physiol., September 1, 2009; 587(17): 4319 - 4327.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Calabria, S. Ciciliot, I. Moretti, M. Garcia, A. Picard, K. A. Dyar, G. Pallafacchina, J. Tothova, S. Schiaffino, and M. Murgia
NFAT isoforms control activity-dependent muscle fiber type specification
PNAS, August 11, 2009; 106(32): 13335 - 13340.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
O. Agbulut, A. Vignaud, C. Hourde, E. Mouisel, F. Fougerousse, G. S. Butler-Browne, and A. Ferry
Slow myosin heavy chain expression in the absence of muscle activity
Am J Physiol Cell Physiol, January 1, 2009; 296(1): C205 - C214.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. W. Tsika, C. Schramm, G. Simmer, D. P. Fitzsimons, R. L. Moss, and J. Ji
Overexpression of TEAD-1 in Transgenic Mouse Striated Muscles Produces a Slower Skeletal Muscle Contractile Phenotype
J. Biol. Chem., December 26, 2008; 283(52): 36154 - 36167.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. A. Rana, K. Gundersen, and A. Buonanno
Activity-dependent repression of muscle genes by NFAT
PNAS, April 15, 2008; 105(15): 5921 - 5926.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. A. Valdes, E. Gaggero, J. Hidalgo, N. Leal, E. Jaimovich, and M. A. Carrasco
NFAT activation by membrane potential follows a calcium pathway distinct from other activity-related transcription factors in skeletal muscle cells
Am J Physiol Cell Physiol, March 1, 2008; 294(3): C715 - C725.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. J. Rose, C. Frosig, B. Kiens, J. F. P. Wojtaszewski, and E. A. Richter
Effect of endurance exercise training on Ca2+ calmodulin-dependent protein kinase II expression and signalling in skeletal muscle of humans
J. Physiol., September 1, 2007; 583(2): 785 - 795.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
S. Schiaffino, M. Sandri, and M. Murgia
Activity-Dependent Signaling Pathways Controlling Muscle Diversity and Plasticity
Physiology, August 1, 2007; 22(4): 269 - 278.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. J. Rose, T. J. Alsted, J. B. Kobbero, and E. A. Richter
Regulation and function of Ca2+-calmodulin-dependent protein kinase II of fast-twitch rat skeletal muscle
J. Physiol., May 1, 2007; 580(3): 993 - 1005.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006