spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 11 Apr 2006
doi: 10.1242/jcs.02894

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02894v1
119/9/1812    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Torres, V. A.
Right arrow Articles by Quest, A. F.G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Torres, V. A.
Right arrow Articles by Quest, A. F.G.

Research Article

Caveolin-1 controls cell proliferation and cell death by suppressing expression of the inhibitor of apoptosis protein survivin


Vicente A. Torres, Julio C. Tapia, Diego A. Rodríguez, Mario Párraga, Pamela Lisboa, Margarita Montoya, Lisette Leyton, and Andrew F.G. Quest*
* Author for correspondence (e-mail: aquest{at}med.uchile.cl)

Caveolin-1 is suggested to act as a tumor suppressor. We tested the hypothesis that caveolin-1 does so by repression of survivin, an Inhibitor of apoptosis protein that regulates cell-cycle progression as well as apoptosis and is commonly overexpressed in human cancers. Ectopic expression of caveolin-1 in HEK293T and ZR75 cells or siRNA-mediated silencing of caveolin-1 in NIH3T3 cells caused downregulation or upregulation of survivin mRNA and protein, respectively. Survivin downregulation in HEK293T cells was paralleled by reduced cell proliferation, increases in G0-G1 and decreases in G2-M phase of the cell cycle. In addition, apoptosis was evident, as judged by several criteria. Importantly, expression of green fluorescent protein-survivin in caveolin-1-transfected HEK293T cells restored cell proliferation and viability. In addition, expression of caveolin-1 inhibited transcriptional activity of a survivin promoter construct in a {beta}-catenin-Tcf/Lef-dependent manner. Furthermore, in HEK293T cells caveolin-1 associated with {beta}-catenin and inhibited Tcf/Lef-dependent transcription. Similar results were obtained upon caveolin-1 expression in DLD1 cells, where APC mutation leads to constitutive activation of {beta}-catenin-Tcf/Lef-mediated transcription of survivin. Taken together, these results suggest that anti-proliferative and pro-apoptotic properties of caveolin-1 may be attributed to reduced survivin expression via a mechanism involving diminished {beta}-catenin-Tcf/Lef-dependent transcription.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Dehner, M. Hadjihannas, J. Weiske, O. Huber, and J. Behrens
Wnt Signaling Inhibits Forkhead Box O3a-induced Transcription and Apoptosis through Up-regulation of Serum- and Glucocorticoid-inducible Kinase 1
J. Biol. Chem., July 11, 2008; 283(28): 19201 - 19210.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
D. Kook, A. H. Wolf, A. L. Yu, A. S. Neubauer, S. G. Priglinger, A. Kampik, and U. C. Welge-Lussen
The Protective Effect of Quercetin against Oxidative Stress in the Human RPE In Vitro
Invest. Ophthalmol. Vis. Sci., April 1, 2008; 49(4): 1712 - 1720.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
S. E. Park, S. H. Jeong, S.-B. Yee, T. H. Kim, Y. H. Soung, N. C. Ha, N. D. Kim, J.-Y. Park, H. R. Bae, B. S. Park, et al.
Interactions of acetylcholinesterase with caveolin-1 and subsequently with cytochrome c are required for apoptosome formation
Carcinogenesis, April 1, 2008; 29(4): 729 - 737.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W. Wrasidlo, A. Mielgo, V. A. Torres, S. Barbero, K. Stoletov, T. L. Suyama, R. L. Klemke, W. H. Gerwick, D. A. Carson, and D. G. Stupack
The marine lipopeptide somocystinamide A triggers apoptosis via caspase 8
PNAS, February 19, 2008; 105(7): 2313 - 2318.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. A. Torres, J. C. Tapia, D. A. Rodriguez, A. Lladser, C. Arredondo, L. Leyton, and A. F. G. Quest
E-Cadherin Is Required for Caveolin-1-Mediated Down-Regulation of the Inhibitor of Apoptosis Protein Survivin via Reduced {beta}-Catenin-Tcf/Lef-Dependent Transcription
Mol. Cell. Biol., November 1, 2007; 27(21): 7703 - 7717.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
N. Odajima, T. Betsuyaku, Y. Nasuhara, and M. Nishimura
Loss of Caveolin-1 in Bronchiolization in Lung Fibrosis
J. Histochem. Cytochem., September 1, 2007; 55(9): 899 - 909.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. C. Tapia, V. A. Torres, D. A. Rodriguez, L. Leyton, and A. F. G. Quest
Casein kinase 2 (CK2) increases survivin expression via enhanced beta-catenin-T cell factor/lymphoid enhancer binding factor-dependent transcription
PNAS, October 10, 2006; 103(41): 15079 - 15084.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006