spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 24 Jul 2008
doi: 10.1242/jcs.029819

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.029819v1
121/16/2696    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kinsey, R.
Right arrow Articles by Kielty, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kinsey, R.
Right arrow Articles by Kielty, C. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Fibrillin-1 microfibril deposition is dependent on fibronectin assembly


Rachel Kinsey, Matthew R. Williamson, Shazia Chaudhry, Kieran T. Mellody, Amanda McGovern, Seiichiro Takahashi, C. Adrian Shuttleworth, and Cay M. Kielty*
* Author for correspondence (e-mail: cay.kielty{at}manchester.ac.uk)

Newly deposited microfibrils strongly colocalise with fibronectin in primary fibroblasts. Microfibril formation is grossly inhibited by fibronectin depletion, but rescued by supplementation with exogenous cellular fibronectin. As integrin receptors are key determinants of fibronectin assembly, we investigated whether they also influenced microfibril deposition. Analysis of {beta}1-integrin-receptor-null fibroblasts, blockage of cell surface integrin receptors that regulate fibronectin assembly and disruption of Rho kinase all result in suppressed deposition of both fibronectin and microfibrils. Antibody activation of {beta}1 integrins in fibronectin-depleted cultures is insufficient to rescue microfibril assembly. In fibronectinRGE/RGE mutant mouse fibroblast cultures, which do not engage {alpha}5{beta}1 integrin, extracellular assembly of both fibronectin and microfibrils is markedly reduced. Thus, pericellular microfibril assembly is regulated by fibronectin fibrillogenesis.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
H.-Y. Chiang, V. A. Korshunov, A. Serour, F. Shi, and J. Sottile
Fibronectin Is an Important Regulator of Flow-Induced Vascular Remodeling
Arterioscler Thromb Vasc Biol, July 1, 2009; 29(7): 1074 - 1079.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
V. Hucthagowder, E. Morava, U. Kornak, D. J. Lefeber, B. Fischer, A. Dimopoulou, A. Aldinger, J. Choi, E. C. Davis, D. N. Abuelo, et al.
Loss-of-function mutations in ATP6V0A2 impair vesicular trafficking, tropoelastin secretion and cell survival
Hum. Mol. Genet., June 15, 2009; 18(12): 2149 - 2165.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Huveneers and E. H. J. Danen
Adhesion signaling - crosstalk between integrins, Src and Rho
J. Cell Sci., April 15, 2009; 122(8): 1059 - 1069.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Zhou, H. Y. Kim, and L. A. Davidson
Actomyosin stiffens the vertebrate embryo during crucial stages of elongation and neural tube closure
Development, February 15, 2009; 136(4): 677 - 688.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2008