spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 8 Aug 2006
doi: 10.1242/jcs.03144

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.03144v1
119/17/3527    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ali, G. S.
Right arrow Articles by Reddy, A. S.N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ali, G. S.
Right arrow Articles by Reddy, A. S.N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

ATP, phosphorylation and transcription regulate the mobility of plant splicing factors


Gul Shad Ali and Annireddy S.N. Reddy*
* Author for correspondence (e-mail: reddy{at}colostate.edu)

Serine-arginine-rich (SR) proteins, a family of spliceosomal proteins, function at multiple steps in the assembly of the spliceosome in non-plant systems. Limited studies with metazoan SR splicing factors (ASF/SF2 and SC35) indicated that their mobility is not dependent on ATP and phosphorylation. In addition, inhibition of transcription slightly increased their mobility. Here, we analyzed the mobility of SR45, a plant-specific SR protein with unique domain organization, and SR1/SRp34, a plant homolog of metazoan ASF/SF2, using fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP). Our results show that, in contrast to metazoan SR splicing factors, the movement of the plant SR proteins is dependent on ATP, phosphorylation and transcription. To understand the underlying mechanism for these observations, we carried out mobility analyses with the domain-deletion mutants of SR45 in ATP-depleted cells and in the presence of inhibitors of transcription or phosphorylation. Our results show that the sensitivity of SR45 to these inhibitors is conferred by an RNA-recognition motif (RRM) and the serine-arginine-rich (RS) domain 2. These results provide important insights into the mechanisms of plant SR protein movement and suggest fundamental differences in the regulation of the mobility of plant and animal SR splicing factors.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
X.-N. Zhang and S. M. Mount
Two Alternatively Spliced Isoforms of the Arabidopsis SR45 Protein Have Distinct Roles during Normal Plant Development
Plant Physiology, July 1, 2009; 150(3): 1450 - 1458.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
O.A. Koroleva, G. Calder, A.F. Pendle, S.H. Kim, D. Lewandowska, C.G. Simpson, I.M. Jones, J.W.S. Brown, and P.J. Shaw
Dynamic Behavior of Arabidopsis eIF4A-III, Putative Core Protein of Exon Junction Complex: Fast Relocation to Nucleolus and Splicing Speckles under Hypoxia
PLANT CELL, May 1, 2009; 21(5): 1592 - 1606.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
R. Wightman, R. Marshall, and S. R. Turner
A Cellulose Synthase-Containing Compartment Moves Rapidly Beneath Sites of Secondary Wall Synthesis
Plant Cell Physiol., March 1, 2009; 50(3): 584 - 594.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G. S. Ali, K. V. S. K. Prasad, I. Day, and A. S. N. Reddy
Ligand-Dependent Reduction in the Membrane Mobility of FLAGELLIN SENSITIVE2, an Arabidopsis Receptor-Like Kinase
Plant Cell Physiol., November 1, 2007; 48(11): 1601 - 1611.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. Tanabe, K. Yoshimura, A. Kimura, Y. Yabuta, and S. Shigeoka
Differential Expression of Alternatively Spliced mRNAs of Arabidopsis SR Protein Homologs, atSR30 and atSR45a, in Response to Environmental Stress
Plant Cell Physiol., July 1, 2007; 48(7): 1036 - 1049.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006