spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 5 Sep 2006
doi: 10.1242/jcs.03160

This Article
Right arrow Full Text (PDF)
Right arrowOA All Versions of this Article:
jcs.03160v1
119/19/3958    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shiraishi, H.
Right arrow Articles by Yoshida, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shiraishi, H.
Right arrow Articles by Yoshida, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

ER stress-induced apoptosis and caspase-12 activation occurs downstream of mitochondrial apoptosis involving Apaf-1


Hiroshi Shiraishi, Hideaki Okamoto, Akihiko Yoshimura, and Hiroki Yoshida*
* Author for correspondence (e-mail: yoshidah{at}med.saga-u.ac.jp)

Accumulation of unfolded proteins induces endoplasmic reticulum (ER) stress. Excessive and prolonged stresses lead cells to apoptosis. However, the precise molecular mechanisms of ER stress-induced apoptosis have not been fully elucidated. We investigated the involvement of the apoptosome in ER stress-induced cell death pathway using mouse embryonic fibroblasts (MEFs) and mice deficient for Apaf-1. Apaf-1-deficient MEFs showed more resistance to ER stress-inducing reagents as compared with wild type cells. Despite comparable induction of ER stress in both wild type and Apaf-1-deficient cells, activation of caspase-3 was only observed in wild type, but not Apaf-1-deficient, MEFs. Under ER stress conditions, BAX translocated to mitochondria and cytochrome c was released from mitochondria. We also demonstrated that caspase-12 was processed downstream of Apaf-1 and caspase-3, and neither overexpression nor knockdown of caspase-12 affected susceptibility of the cells to ER stress-induced cell death. Furthermore, in the kidneys of Apaf-1-deficient mice, apoptosis induced by in vivo administration of tunicamycin was remarkably suppressed as compared with wild type mice. These data collectively demonstrated that Apaf-1 and the mitochondrial pathway of apoptosis play significant roles in ER stress-induced apoptosis.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
M. F. Tonnesen, L. G. Grunnet, J. Friberg, A. K. Cardozo, N. Billestrup, D. L. Eizirik, J. Storling, and T. Mandrup-Poulsen
Inhibition of Nuclear Factor-{kappa}B or Bax Prevents Endoplasmic Reticulum Stress- But Not Nitric Oxide-Mediated Apoptosis in INS-1E Cells
Endocrinology, September 1, 2009; 150(9): 4094 - 4103.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
G. Matsuura, N. Morinaga, K. Yahiro, R. Komine, J. Moss, H. Yoshida, and M. Noda
Novel Subtilase Cytotoxin Produced by Shiga-Toxigenic Escherichia coli Induces Apoptosis in Vero Cells via Mitochondrial Membrane Damage
Infect. Immun., July 1, 2009; 77(7): 2919 - 2924.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
E. Szegezdi, D. C. MacDonald, T. Ni Chonghaile, S. Gupta, and A. Samali
Bcl-2 family on guard at the ER
Am J Physiol Cell Physiol, May 1, 2009; 296(5): C941 - C953.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Lei, S. Zhang, A. Bohrer, and S. Ramanadham
Calcium-independent Phospholipase A2 (iPLA2{beta})-mediated Ceramide Generation Plays a Key Role in the Cross-talk between the Endoplasmic Reticulum (ER) and Mitochondria during ER Stress-induced Insulin-secreting Cell Apoptosis
J. Biol. Chem., December 12, 2008; 283(50): 34819 - 34832.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
L.-p. Yang, L.-m. Wu, X.-j. Guo, and M. O. M. Tso
Activation of Endoplasmic Reticulum Stress in Degenerating Photoreceptors of the rd1 Mouse
Invest. Ophthalmol. Vis. Sci., November 1, 2007; 48(11): 5191 - 5198.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Guo, T. Zhu, Z.-X. J. Xiao, and C.-Y. Chen
Modulation of Intracellular Signaling Pathways to Induce Apoptosis in Prostate Cancer Cells
J. Biol. Chem., August 17, 2007; 282(33): 24364 - 24372.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Masud, A. Mohapatra, S. A. Lakhani, A. Ferrandino, R. Hakem, and R. A. Flavell
Endoplasmic Reticulum Stress-induced Death of Mouse Embryonic Fibroblasts Requires the Intrinsic Pathway of Apoptosis
J. Biol. Chem., May 11, 2007; 282(19): 14132 - 14139.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006