spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 23 Jan 2007
doi: 10.1242/jcs.03368

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.03368v1
120/4/586    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bryja, V.
Right arrow Articles by Arenas, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bryja, V.
Right arrow Articles by Arenas, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Wnt-5a induces Dishevelled phosphorylation and dopaminergic differentiation via a CK1-dependent mechanism


Vítezslav Bryja, Gunnar Schulte, Nina Rawal, Alexandra Grahn, and Ernest Arenas*
* Author for correspondence (e-mail: ernest.arenas{at}ki.se)

Previously, we have shown that Wnt-5a strongly regulates dopaminergic neuron differentiation by inducing phosphorylation of Dishevelled (Dvl). Here, we identify additional components of the Wnt-5a-Dvl pathway in dopaminergic cells. Using in vitro gain-of-function and loss-of-function approaches, we reveal that casein kinase 1 (CK1) {delta} and CK1{epsilon} are crucial for Dvl phosphorylation by non-canonical Wnts. We show that in response to Wnt-5a, CK1{epsilon} binds Dvl and is subsequently phosphorylated. Moreover, in response to Wnt-5a or CK1{epsilon}, the distribution of Dvl changed from punctate to an even appearance within the cytoplasm. The opposite effect was induced by a CK1{epsilon} kinase-dead mutant or by CK1 inhibitors. As expected, Wnt-5a blocked the Wnt-3a-induced activation of {beta}-catenin. However, both Wnt-3a and Wnt-5a activated Dvl2 by a CK1-dependent mechanism in a cooperative manner. Finally, we show that CK1 kinase activity is necessary for Wnt-5a-induced differentiation of primary dopaminergic precursors. Thus, our data identify CK1 as a component of Wnt-5a-induced signalling machinery that regulates dopaminergic differentiation, and suggest that CK1{delta}/{epsilon}-mediated phosphorylation of Dvl is a common step in both canonical and non-canonical Wnt signalling.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. J. Schmeisser, A. M. Grabrucker, J. Bockmann, and T. M. Boeckers
Synaptic Cross-talk between N-Methyl-D-aspartate Receptors and LAPSER1-{beta}-Catenin at Excitatory Synapses
J. Biol. Chem., October 16, 2009; 284(42): 29146 - 29157.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
R. M. Sancho, B. M.H. Law, and K. Harvey
Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways
Hum. Mol. Genet., October 15, 2009; 18(20): 3955 - 3968.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. M. Walton, K. Fisher, D. Rubitski, M. Marconi, Q.-J. Meng, M. Sladek, J. Adams, M. Bass, R. Chandrasekaran, T. Butler, et al.
Selective Inhibition of Casein Kinase 1{epsilon} Minimally Alters Circadian Clock Period
J. Pharmacol. Exp. Ther., August 1, 2009; 330(2): 430 - 439.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Medrek, G. Landberg, T. Andersson, and K. Leandersson
Wnt-5a-CKI{alpha} Signaling Promotes {beta}-Catenin/E-Cadherin Complex Formation and Intercellular Adhesion in Human Breast Epithelial Cells
J. Biol. Chem., April 17, 2009; 284(16): 10968 - 10979.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
D.-H. Yang, J.-Y. Yoon, S.-H. Lee, V. Bryja, E. R. Andersson, E. Arenas, Y.-G. Kwon, and K.-Y. Choi
Wnt5a Is Required for Endothelial Differentiation of Embryonic Stem Cells and Vascularization via Pathways Involving Both Wnt/{beta}-Catenin and Protein Kinase C{alpha}
Circ. Res., February 13, 2009; 104(3): 372 - 379.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Andersson, E. Sodersten, J. K. Duckworth, A. Cascante, N. Fritz, P. Sacchetti, I. Cervenka, V. Bryja, and O. Hermanson
CXXC5 Is a Novel BMP4-regulated Modulator of Wnt Signaling in Neural Stem Cells
J. Biol. Chem., February 6, 2009; 284(6): 3672 - 3681.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
V. Bryja, E. R. Andersson, A. Schambony, M. Esner, L. Bryjova, K. K. Biris, A. C. Hall, B. Kraft, L. Cajanek, T. P. Yamaguchi, et al.
The Extracellular Domain of Lrp5/6 Inhibits Noncanonical Wnt Signaling In Vivo
Mol. Biol. Cell, February 1, 2009; 20(3): 924 - 936.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Gelebart, M. Anand, H. Armanious, A. C. Peters, J. Dien Bard, H. M. Amin, and R. Lai
Constitutive activation of the Wnt canonical pathway in mantle cell lymphoma
Blood, December 15, 2008; 112(13): 5171 - 5179.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. Roybon, T. Hjalt, N. S. Christophersen, J.-Y. Li, and P. Brundin
Effects on Differentiation of Embryonic Ventral Midbrain Progenitors by Lmx1a, Msx1, Ngn2, and Pitx3
J. Neurosci., April 2, 2008; 28(14): 3644 - 3656.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Y. Sokol and K. A. Wharton Jr
WNTers in La Jolla
Development, October 1, 2007; 134(19): 3393 - 3399.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Bryja, D. Gradl, A. Schambony, E. Arenas, and G. Schulte
beta-Arrestin is a necessary component of Wnt/beta-catenin signaling in vitro and in vivo
PNAS, April 17, 2007; 104(16): 6690 - 6695.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007