Subject collection: Autophagy
- New insights into autophagosome–lysosome fusion
Summary: Autophagy is a conserved intracellular degradation system. Here, we summarize the current knowledge of autophagosome–lysosome fusion and discuss the remaining questions of the field.
- Ratiometric analysis of Acridine Orange staining in the study of acidic organelles and autophagy
Summary: We describe the photochemical basis of Acridine Orange (AO) and its usefulness as a probe for autophagy quantification and find that considering the red-to-green fluorescence intensity ratio of AO gives the best correlation with other autophagy assays.
- TRIM17 contributes to autophagy of midbodies while actively sparing other targets from degradation
Highlighted Article: TRIM17 regulates selective autophagy by inhibiting the degradation of a range of substrates but promoting the autophagic removal of midbodies. Thus TRIM17 facilitates the precise discrimination between potential autophagy substrates.
- TFEB at a glance
Summary: The article discusses the roles of TFEB as a regulator of lysosomal biogenesis and intracellular clearance, and its involvement in human diseases.
- Danon disease – dysregulation of autophagy in a multisystem disorder with cardiomyopathy
Summary: Danon disease is a severe form of cardiomyopathy, and the molecular mechanisms involved in progression of the disease are poorly understood; however, perturbation of autophagy is likely to play a crucial role.
- Distinguishing aggregate formation and aggregate clearance using cell-based assays
Highlighted Article: Disequilibrium between aggregate formation and clearance leads to accumulation of aggregated proteins. We have created a new cell-based assay that can determine how modifiers can impact on aggregate burden.
- Precision autophagy directed by receptor regulators – emerging examples within the TRIM family
Summary: Precision autophagy represents direct recognition of targets by receptors, such as TRIMs, that simultaneously organize core autophagy proteins, coupling recognition to autophagosome formation.
- Coordination of autophagosome–lysosome fusion and transport by a Klp98A–Rab14 complex in Drosophila
Summary: Autophagic degradation requires trafficking and fusion of autophagosomes and lysosomes. Here, Rab14 and the PX-domain-containing kinesin Klp98A are shown to promote vesicle formation, transport and fusion.
- The Ccl1–Kin28 kinase complex regulates autophagy under nitrogen starvation
Summary: Screening of known kinases in the yeast genome revealed the Ccl1–Kin28 complex as a novel regulator of autophagy. Proteasomal turnover of Ccl1 limits autophagy during prolonged starvation.
- Sqstm1–GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells
Highlighted Article: We have developed Sqstm1-GFP knock-in mice, which are useful for elucidating the molecular mechanisms of cellular events, including autophagy, and various diseases in which Sqstm1 plays a role.