Subject collection: Cilia and Flagella
- Intraflagellar transport protein IFT52 recruits IFT46 to the basal body and flagella
Summary: We identify the region in IFT46 that is responsible for its basal body and ciliary localization. This sequence promotes interaction with IFT52, which recruits IFT46 to the basal body.
- The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking
Summary: The retinal degeneration gene product CCDC66 is a microtubule-associated protein that localizes to the centrosome and cilium complex, and functions in cilium formation and recruitment of BBS4 to the cilium.
- IFT57 stabilizes the assembled intraflagellar transport complex and mediates transport of motility-related flagellar cargo
Highlighted Article: Intraflagellar transport (IFT) protein IFT57 maintains the pool size of IFT particles for ciliogenesis by stabilizing assembled IFT particle complexes, and is critical for flagellar waveform establishment.
- Regulation of ciliary retrograde protein trafficking by the Joubert syndrome proteins ARL13B and INPP5E
Summary: Mutations in the genes encoding ARL13B and INPP5E are causative for Joubert syndrome. ARL13B interacts with INPP5E and regulates IFT-A-mediated retrograde protein trafficking within cilia.
- Intraflagellar transport is required for the maintenance of the trypanosome flagellum composition but not its length
Summary: Intraflagellar transport machinery is responsible for the construction of cilia and flagella. We show that in trypanosomes it is required for the maintenance of flagellum integrity but not of organelle length.
- Deleterious assembly of the lamin A/C mutant p.S143P causes ER stress in familial dilated cardiomyopathy
Highlighted Article: Mutant p.S143P lamin A/C causes familial dilated cardiomyopathy (DCM). It is shown that this protein affects normal lamina structure and influences the cellular stress response, homeostasis and viability.
- Ttll9−/− mice sperm flagella show shortening of doublet 7, reduction of doublet 5 polyglutamylation and a stall in beating
Highlighted Article: A tubulin polyglutamylase, TTLL9, is involved in the establishment of normal interdoublet heterogeneity of sperm axoneme, and is required for structural integrity and fluid motility of murine sperm flagella.
- Two classes of short intraflagellar transport train with different 3D structures are present in Chlamydomonas flagella
Highlighted article: In-depth analyses of IFT train ultrastructure in regenerating Chlamydomonas flagella shows the occurrence of three train types with different 3D structure, implicating differences in function.
- Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin
Summary: Tubulin transport is specifically impaired by mutations to the proposed tubulin-binding domains of the intraflagellar transport proteins IFT81 and IFT74.
- Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity
Highlighted Article: Bioactive peptide amidation is catalyzed by peptidylglycine α-amidating monooxygenase (PAM). Functional PAM is present in Chlamydomonas and cilia, organelles that play key roles in signaling.