Subject collection: Exploring the Nucleus
- Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena
Summary: There are compositional and structural differences in the nuclear pore complexes present in the functionally differentiated macronucleus and micronucleus within the single cytoplasm of ciliated protozoa.
- Nuclear α-catenin mediates the DNA damage response via β-catenin and nuclear actin
Highlighted Article: The nuclear α-catenin–β-catenin complex mediates DNA damage sensitivity in a WNT-dependent manner. α-Catenin recruitment to sites of DNA damage is dependent on β-catenin and nuclear actin.
- TSSK6 is required for γH2AX formation and the histone-to-protamine transition during spermiogenesis
Highlighted Article: Spermatid protein kinase TSSK6 is essential for fertility. Here, we show that TSSK6 is necessary for H2AX phosphorylation at Ser139 and histone-to-protamine transition during spermiogenesis, and link γH2AX to normal sperm development.
- MacroH2A histone variants maintain nuclear organization and heterochromatin architecture
- Non-coding Y RNAs associate with early replicating euchromatin in concordance with the origin recognition complex
Highlighted Article: Non-coding Y RNAs are essential for DNA replication. We show that they associate with euchromatin in a cell cycle-regulated manner in concordance with the origin recognition complex, suggesting a concerted function.
- Super-resolution mapping of scaffold nucleoporins in the nuclear pore complex
Summary: Combination of super-resolution SPEED microscopy and nanobody specific labeling of nucleoporins can be used to map the radial and axial distributions of scaffold proteins in native nuclear pore complexes.
- Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage
Summary: Accumulation of NIPBL, the cohesin loader, at different types of DNA lesions occurs through independent mechanisms. This finding enhances our understanding of the highly dynamic roles for NIPBL in genome stability.
- Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development
Highlighted Article: During neuronal maturation, adenosine-to-inosine RNA editing is regulated by nuclear import of the ADAR2 editing enzyme, which is governed by importin-α4, and nuclear stabilization of ADAR2 by Pin1.
- The LINC complex contributes to heterochromatin organisation and transcriptional gene silencing in plants
Summary: The plant LINC complex contributes to heterochromatin position at the nuclear periphery, maintains transcriptional gene silencing and participates in compaction of heterochromatic sequences in cotyledon epidermis and root hair cells.
- Vesicular PtdIns(3,4,5)P3 and Rab7 are key effectors of sea urchin zygote nuclear membrane fusion
Summary: Class-I PI3-kinases are needed for pronuclear envelope formation and fusion in sea urchin zygotes, and Rab7 GTPase and PtdIns(3,4,5)P3 directly interact during the membrane fusion process.