Protein-kinase-C-mediated β-catenin phosphorylation negatively regulates the Wnt/β-catenin pathway

Normally, the Wnt/β-catenin pathway controls developmental processes and homeostasis, but abnormal activation of this pathway is a frequent event during the development of cancer. The key mechanism in regulation of the Wnt/β-catenin pathway is the amino-terminal phosphorylation of β-catenin, marking it for proteasomal degradation. Here we present small-molecule-based identification of protein kinase C (PKC)-mediated β-catenin phosphorylation as a novel mechanism regulating the Wnt/β-catenin pathway. We used a cell-based chemical screen to identify A23187, which inhibits the Wnt/β-catenin pathway. PKC was activated by A23187 treatment and subsequently phosphorylated N-terminal serine (Ser) residues of β-catenin, which promoted β-catenin degradation. Moreover, the depletion of PKCα inhibited the phosphorylation and degradation of β-catenin. Therefore, our findings suggest that the PKC pathway negatively regulates the β-catenin level outside of the Wnt/β-catenin pathway.


Introduction
Wnts are secreted glycoproteins that play important roles in cell proliferation, differentiation and oncogenesis Morin et al., 1997;Wodarz and Nusse, 1998;Polakis, 2000). The Wnt/b-catenin pathway is activated by Wnts (Wnt1, 3a and 8) interacting with Frizzled (Fz) receptors and lowdensity lipoprotein receptor-related protein5/6 (LRP5/6) coreceptors (Giles et al., 2003). The signal is then transduced through the disheveled protein to negatively regulate glycogen synthase kinase-3b (GSK-3b), resulting in the accumulation of cytoplasmic b-catenin (Lee et al., 2003;Dominguez and Green, 2000). This b-catenin is translocated into the nucleus, where it forms a complex with the T cell factor/lymphocyte enhancer factor (TCF/LEF) families of transcription factors to activate the expression of Wnt/b-catenin responsive genes, such as c-myc, cyclin D1 and peroxisome proliferator-activated receptor-d (He et al., 1998;Tetsu and McCormick, 1999;He et al., 1999). In the absence of a Wnt signal, b-catenin forms a complex with adenomatous polyposis coli (APC)/Axin and is phosphorylated by a dual-kinase mechanism, which is catalyzed by casein kinase 1 (CK1) and GSK-3b, at Ser/Thr residues (Ser33, Ser37, Thr41 and Ser45) (Liu et al., 2002;Amit et al., 2002). These phospho-Ser/Thr residues are recognized by F-box btransducin repeat-containing protein (b-TrCP), an F-box protein in the ubiquitin ligase complex, leading to the degradation of b-catenin (Latres et al., 1999). Thus, b-catenin phosphorylation is the key regulatory mechanism in the Wnt/b-catenin pathway. In this study, we used a small molecule-based approach to explore the mechanisms regulating the Wnt/b-catenin pathway. Our strategy was to use a cell-based chemical screen to identify a small molecule capable of modulating the Wnt/b-catenin pathway and to find the mode of action of this small molecule to determine the mechanism that regulates the Wnt/b-catenin pathway.

Identification of a small-molecule inhibitor of the Wnt/bcatenin pathway
To screen for small molecules that modulate the Wnt/b-catenin pathway, we stably transfected HEK293 cells with the TOPFlash reporter plasmid and the human Frizzled-1 expression plasmid to create HEK293 reporter cells (Gwak et al., 2006;Park et al., 2006). When HEK293 reporter cells were incubated with Wnt3a-conditioned medium (Wnt3a-CM), TOPFlash reporter activity increased dramatically (data not shown). Using this system, we screened a library of small molecules that contained 960 bioactive compounds (Fig. 1A). One of the compounds identified was A23187, a calcium ionophore. As shown in Fig. 1B  Therefore, our findings suggest that the PKC pathway control cells   (Fig. 1B). Under these conditions, A23187 exhibited no cytotoxic effects on the HEK293 reporter cells (Fig. S2 in supplementary material).
These results indicate that A23187 specifically inhibits CRT stimulated by Wnt3a.
In the Wnt/b-catenin pathway, CRT is largely dependent on the level of cytoplasmic b-catenin, which stimulates target genes. Therefore, we investigated the effect of A23187 on cytoplasmic b-catenin levels by Western blotting. Consistent with a previous report (Shibamoto et al., 1998), Western blotting with anti-b-catenin antibody revealed that incubation with Wnt3a-CM increased the amount of b-catenin ( Fig. 1C; Since the level of cytoplasmic b-catenin is regulated by the ubiquitin-dependent proteasome pathway (Latres et al., 1999), we next explored whether A23187-induced downregulation of b-catenin is mediated by proteasomes. When we used MG-132 to block proteasome-mediated protein degradation in HEK293 reporter cells, it abrogated the ability of A23187 to effect a consistent decrease in b-catenin (Fig. 1D), indicating that A23187 promotes the degradation of b-catenin in a proteasome-dependent manner. Taken together, these results indicate that A23187 inhibits the Wnt/b-catenin pathway by a mechanism involving degradation of intracellular b-catenin.
A23187-mediated b-catenin degradation requires the bcatenin N-terminus but not GSK-3b activity Since the phosphorylation of b-catenin by GSK-3b and its subsequent association with b-TrCP leads to b-catenin degradation (Liu et al., 1999;Winston et al., 1999), we examined whether A23187-mediated inhibition of CRT requires GSK-3b activity. To this end, HEK293 reporter cells were incubated with A23187 and LiCl, an inhibitor of GSK-3b (Klein and Melton, 1996). As shown in Fig. 2A, A23187 suppressed LiCl-induced CRT. Furthermore, Western blot analysis using anti-b-catenin antibody consistently showed that A23187 reduced the level of b-catenin that accumulated with LiCl treatment (Fig. 2B), indicating that A23187mediated inhibition of the Wnt/b-catenin pathway is independent of GSK-3b.
We next examined whether b-TrCP is necessary for induction of b-catenin degradation by A23187. Western blot analysis showed that a dominant negative mutant b-TrCP (Db-TrCP), which interacts with phosphorylated b-catenin but is unable to form an SCF b-TrCP -ubiquitin ligase complex (Hart et al., 1999), abrogated induction of b-catenin degradation by A23187 (Fig. 2C). Consistent with this result, A23187mediated CRT suppression was also blocked by Db-TrCP We then examined the effect of A23187 treatment on HEK293 cells transfected with wild-type b-catenin or with a mutant b-catenin lacking the N-terminal phosphorylation motifs (Tetsu and McCormick, 1999). We found that wild-type b-catenin was efficiently downregulated in response to A23187 (Fig. 3A), whereas the level of Db-catenin was largely unaffected by A23187 treatment (Fig. 3B). In addition, A23187 did not affect the stability of another b-catenin mutant, S37A (Fig. 3C). Taken together, these results indicate that Nterminal residues of b-catenin are required for A23187mediated b-catenin downregulation. (DMSO) or A23187 (2.5 mM) in the presence or absence of Wnt3a-CM, exposed to MG-132 (20 mM) for 8 hours, and then subjected to western blotting with anti-b-catenin antibody. In C and D, the blots were reprobed with anti-actin antibody as a loading control.
A23187-mediated degradation of b-catenin requires protein kinase C activity To better understand the mechanism by which A23187 acts to induce b-catenin degradation, we first examined its effect on the intracellular Ca 2+ concentration. When we measured intracellular Ca 2+ using the Ca 2+ indicator fura-2 acetoxymethyl ester (Fura-2/AM), we found that treatment with A23187 dramatically increased the intracellular Ca 2+ concentration in HEK293 reporter cells in the presence of Wnt3a (Fig. 4A). An elevation in the intracellular Ca 2+ concentration has been reported to activate Ca 2+ -sensitive proteins, including protein kinase C (PKC), Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) and calcineurin (Saneyoshi et al., 2002;Sheldahl et al., 2003). To identify the protein required for inhibition of the Wnt/b-catenin pathway in response to an increase in intracellular Ca 2+ , we tested the effects of specific pharmacological inhibitors of these proteins on the restoration of A23187-mediated inhibition of the Wnt/b-catenin pathway. Cyclosporin A (CsA) and KN-93, which inhibit CaMKII and Journal of Cell Science 119 (22) calcineurin, respectively, did not rescue the CRT inhibition by A23187 (Fig. 4B), suggesting that CaMKII and calcineurin are not required for A23187mediated inhibition of the Wnt/b-catenin pathway. By contrast, bisindoylmaleimide (BIM), a specific inhibitor of PKC (Martiny- Baron et al., 1993), abrogated the suppression of CRT by A23187 in a dose-dependent manner (Fig. 4B). Moreover, when incubated with HEK293 reporter cells, it inhibited the degradation of b-catenin induced by A23187 (Fig. 4C).
We also tested the effects of inhibitors of protein kinase A (PKA) and casein kinase1a (CK1a), which have been known to phosphorylate the Ser45 residue of b-catenin (Amit et al., 2002;Kang et al., 2002;Liu et al., 2002), on A23187mediated CRT inhibition. These inhibitors did not abrogate A23187-mediated CRT inhibition (data not shown). Therefore, PKC, which is induced by an increase in intracellular Ca 2+ , may be responsible for inhibiting the Wnt/b-catenin pathway.
PKC is essential for phosphorylation and degradation of b-catenin We next examined whether PKC is activated by treatment with A23187 in HEK293 reporter cells. Since activated PKC translocates from the cytoplasm to the plasma membrane (Newton, 1995), we isolated the membrane fraction from A23187-treated and untreated cells and then measured the amount of PKC protein, using anti-PKCa antibody. Consistent with results reported for other cells (Chakraborti et al., 2004), A23187 treatment led to the translocation of PKCa to the membrane (Fig. 5A). We also observed A23187-induced membrane translocation of PKCa by immunofluorescence  analysis (Fig. 5B). These results suggest that A23187 treatment activates PKCa.
Previous reports have demonstrated that phosphorylation at the N-terminal Ser residues (Ser33/Ser37/Ser45) of b-catenin plays an important role in its ubiquitin-dependent degradation (Liu et al., 2002;Amit et al., 2002). Therefore, to examine whether PKC directly phosphorylates these residues, we performed an in vitro kinase assay using purified b-catenin and PKC. Phosphorylation was analyzed using phospho-specific bcatenin antibodies. As shown in Fig. 6A, Ser33/Ser37/Ser45 phosphorylation was catalyzed by PKC in a dose-dependent manner. Moreover, BIM treatment inhibited this phosphorylation (Fig. 6B).
We also examined whether PKC activity is responsible for phosphorylation of b-catenin Ser residues in HEK293 reporter cells. In agreement with previous reports (Liu et al., 2002;Amit et al., 2002), western blot analysis showed that Wnt3a inhibited phosphorylation of b-catenin residues Ser33/Ser37/Ser45 (Fig. 7A). Moreover, A23187 induced Ser33/Ser37/Ser45 phosphorylation, and this phosphorylation was abrogated by the addition of BIM (Fig.  7A). These results suggest that PKC, which is activated by an increase in intracellular Ca 2+ , phosphorylates N-terminal Ser residues of b-catenin, thereby promoting b-catenin degradation in vivo.
To confirm that PKC can mediate phosphorylation of Ser33/Ser37/Ser45 of b-catenin, we depleted the endogenous PKCa protein in HEK293 cells using small interference RNA (siRNA). PKCa siRNA significantly reduced the level of PKCa and inhibited Ser33/Ser37/Ser45 phosphorylation (Fig.  7B). Notably, PKCa siRNA also led to significant accumulation of b-catenin protein (Fig. 7B). Consistent with our western blotting results, CRT was markedly increased by transfection with PKCa siRNA (Fig. 7C).
PKC is partially involved in Wnt5a-mediated inhibition of the Wnt/b-catenin pathway Since Wnt5a may trigger release of intracellular Ca 2+ and activate Ca 2+ -responsive enzymes (Kuhl et al., 2000b), we examined whether Wnt5a also suppresses Wnt3a-induced CRT via a PKC-dependent mechanism. Consistent with a previous report (Topol et al., 2003), Wnt5a antagonized the Wnt/bcatenin pathway that is stimulated by Wnt3a (Fig. 8A). In addition, inhibition of PKC activity with BIM only partially abolished Wnt5a-mediated CRT inhibition (Fig. 8B). These results suggest that the inhibition of the Wnt/b-catenin pathway by Wnt5a may be mediated by a mechanism other than the PKC-dependent mechanism.

Discussion
The Wnt pathway regulates a variety of developmental processes, including cell proliferation and cell-fate specification Morin et al., 1997;Wodarz and Nusse, 1998). Inappropriate upregulation of this pathway is associated with the development of certain human cancers (Polakis, 2000). Central to the Wnt/b-catenin pathway is the level of cytosolic b-catenin, which is regulated by phosphorylation and subsequent ubiquitin-dependent proteolysis (Liu et al., 1999;Winston et al., 1999). In the present study, we exploited a small-molecule-based approach in demonstrating that PKC is a negative regulator of the Wnt/bcatenin pathway. The small molecule A23187 was found to inhibit the Wnt/b-catenin pathway. A23187 increased the intracellular Ca 2+ level and subsequently stimulated PKC, which phosphorylated the N-terminal Ser residues of bcatenin, leading to the promotion of b-catenin degradation.
The level of intracellular b-catenin is regulated by multiple pathways. In the GSK-3b-dependent pathway, N-terminal serines of b-catenin are phosphorylated by a multi-protein complex composed of APC, Axin and GSK-3b (Hart et al., Journal of Cell Science 119 (22) 1998; Ikeda et al., 1998). This phosphorylation marks the bcatenin protein for degradation by an ubiquitin-dependent mechanism (Aberle et al., 1997). In the Siah-1-dependent pathway, Siah-1 interacts with the carboxyl terminus of APC, recruits the ubiquitination complex, and promotes the degradation of b-catenin through a pathway independent of both GSK-3b and b-TrCP (Liu et al., 2001;Matsuzawa and Read, 2001). In addition to the two APC-dependent pathways, b-catenin is downregulated by a retinoid X receptor (RXR)mediated degradation pathway that does not target the Nterminus of b-catenin (Xiao et al., 2003).
Several findings of the present study suggest that a novel degradation pathway, other than the above-described pathways, may mediate induction of b-catenin downregulation by A23187. First, A23187 was able to stimulate b-catenin degradation in the presence of the GSK-3b inhibitors Wnt3a and LiCl (Klein and Melton, 1996;Liu et al., 2002), demonstrating that A23187-mediated b-catenin degradation is GSK-3b-independent. Second, Db-TrCP (F-box deletion mutant) nullified the effect of A23187 on the level of intracellular b-catenin, suggesting that A23187 promotes bcatenin degradation through a b-TrCP-dependent proteasome pathway. Third, since the N-terminus of b-catenin was indispensable for the downregulatory activity of A23187 (Fig.  3B), A23187 does not exert its effect through the RXRmediated degradation pathway. Fourth, the b-catenin mutant S37A was not degraded in response to A23187, implying that phosphorylation of b-catenin at Ser37 may be essential for A23187-mediated b-catenin degradation.
Since A23187 is a Ca 2+ ionophore that increases the intracellular Ca 2+ level, it may activate one or more Ca 2+dependent proteins, such as CaMKII, calcineurin or PKC (Sheldahl et al., 2003;Saneyoshi et al., 2002). Specific pharmacological inhibitors of CaMKII and calcineurin did not inhibit downregulation of b-catenin levels by A23187,  revealing that activation of NF-AT or CaMKII is not required for the antagonistic activity of A32187 toward the Wnt/bcatenin pathway. By contrast, the specific PKC inhibitor BIM rescued induction of b-catenin degradation by A23187.
Moreover, Western blot and immunofluorescence analysis showed that endogenous PKCa is activated by A23187 treatment. These results suggest that PKC may be involved in Previous studies have suggested a potential role for PKC in the Wnt/b-catenin pathway. Cook and colleagues reported that PKC inhibitors abrogate Wnt-mediated GSK-3b inhibition in mouse 10T1/2 fibroblasts (Cook et al., 1996), but they did not examine the effect of PKC inhibitors on the intracellular bcatenin level. Others have demonstrated that treatment with the PKC inhibitors cause b-catenin accumulation in human breast cell lines in the absence of Wnt activation (Orford et al., 1997). In the present study, an in vitro kinase assay showed that PKC directly phosphorylates residues Ser33/Ser37/Ser45 of b-catenin in the presence of Ca 2+ and lipid activator. Most importantly, depletion of endogenous PKCa with siRNA prevented Ser33/Ser37/Ser45 phosphorylation and degradation, implying that PKCa suppresses the Wnt/b-catenin pathway in response to a change in the intracellular Ca 2+ concentration through the phosphorylation and subsequent degradation of b-catenin.
Several studies have suggested a dual kinase mechanism in which phosphorylation of b-catenin at Ser45 by CK1 is essential for subsequent GSK-3b-mediated phosphorylation of the N-terminal Ser residues in a complex with APC and Axin (Amit et al., 2002;Liu et al., 2002). Parallel to GSK-3bmediated b-catenin phosphorylation, PKC could mediate phosphorylation of b-catenin at both Ser33/37 and Ser 45 without requiring a priming kinase such as CK1a or PKA.
Wnt5a may also activate PKC by triggering the release of intracellular Ca 2+ (Kuhl et al., 2000a). Moreover, Wnt/bcatenin is antagonized by the Wnt/Ca 2+ pathways (Ishitani et al., 2003;Kremenevskaja et al., 2005;Kuhl et al., 2000b). Thus, Wnt5a might plausibly attenuate the Wnt/b-catenin pathway by activating PKC. However, we found that BIM could not completely rescue the Wnt5a-mediated suppression of the Wnt/b-catenin pathway, indicating that this suppression may be mediated by a mechanism distinct from the PKC-  dependent mechanism. This result can be explained by the earlier observation that Wnt5a promotes b-catenin degradation via a mechanism that is dependent on Siah/APC but not on Ca 2+ -responsive enzymes (Topol et al., 2003).
In conclusion, we have evaluated a possible mechanism for Ca 2+ -mediated inhibition of the Wnt/b-catenin pathway in mammals using cell-based small molecule screening. Since aberrant upregulation of b-catenin levels and subsequent activation of CRT is associated with the development of certain cancers, our findings may facilitate the development of new strategies to prevent tumorigenesis.

Materials and Methods
Cell culture, transfection, siRNA and luciferase assay HEK293 and Wnt3a-secreting L cells were obtained from the American Type Culture Collection and maintained in DMEM supplemented with 10% fetal bovine serum (FBS), 120 mg/ml penicillin, and 200 mg/ml streptomycin. For Wnt3aconditioned medium (Wnt3a-CM), Wnt3a-secreting L cells were cultured in DMEM with 10% FBS for 4 days. The medium was harvested and sterilized using a 0.22-mm filter. Fresh medium was added, and the cells were cultured for another 3 days. The medium was collected and combined with the previous medium. Transfection was carried out with Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. PKCa siRNA was designed as previously described (Chen et al., 2004) and synthesized by Invitrogen. Negative control siRNA (Silencer TM ) was purchased from Ambion. Luciferase assays were performed using the Dual luciferase assay kit (Promega).

Plasmid and recombinant proteins
Human Frizzled cDNA was cloned as described previously (Cho et al., 2005). The pTOPFlash and pFOPFlash reporter plasmids were obtained from Upstate Biotechnology (Lake Placid, NY, USA). The dominant negative b-TrCP expression plasmid was kindly provided by Dr M. Davis (Hebrew University-Hadassah Medical School, Israel), and pGEX-4T-1-b-catenin plasmid was a gift from Dr W. Song (GIST, Korea). The recombinant protein was purified as previously described (Park et al., 2004).

Screening for small-molecule inhibitors of Wnt/b-catenin signaling
The HEK293 reporter cell line was established as previously described (Gwak et al., 2006;Park et al., 2006). The cells were inoculated into 96-well plates at 15,000 cells per well in duplicate and grown for 24 h. Next, Wnt3a-CM was added, and then the chemicals (Genesis Plus Collection, MicroSource Discovery Inc., Gaylordsville, CT) were added to the wells at a final concentration of 10 mM. After 15 hours, the plates were assayed for firefly luciferase activity and cell viability.
Preparing the membrane fraction HEK293 reporter cells grown in 100-mm culture dishes were washed with ice-cold PBS. The cells were then scraped from the dish; suspended in 1 ml of ice-cold extraction buffer containing 20 mM Tris (pH 7.5), 0.5 mM EDTA, and 0.5 mM EGTA; homogenized using a syringe; and incubated on ice for 30 minutes. The homogenate was centrifuged at 13,400 g for 2 minutes at 4°C. The supernatant was centrifuged at 100,000 g for 30 minutes at 4°C in a 100Ti rotor (Beckman, USA). The pellet was suspended in 0.3% (w/v) Triton X-100. The membrane suspension was aliquoted and stored at -80°C.

In vitro kinase assay
Kinase assays were performed with a PKC assay kit (Upstate Cell Signaling Solutions) according to the manufacturer's instructions with minor modifications. Briefly, purified GST-b-catenin (100 ng) was incubated with PKC (Promega) at 30°C for 30 minutes in kinase assay buffer (20 mM Mops, pH 7.2, 25 mM bglycerol phosphate, 1 mM sodium orthovanadate, 1 mM dithiothreitol, 1 mM CaCl 2 , PKA/CaMK inhibitor cocktail, PKC lipid activator, magnesium/ATP cocktail). The proteins were subjected to SDS-PAGE and transferred onto nitrocellulose membranes. The transferred proteins were analyzed using western blotting with anti-phospho-b-catenin antibody, and the membrane was exposed to X-ray film.

Measuring Ca 2+ concentration
Ca 2+ levels in the cells were determined using the fluorescent probe Fura-2 AM (Molecular Probes). HEK293 reporter cells were incubated in Wnt3a-CM medium containing 2.5 mM A23187. The cells were fixed in 4% paraformaldehyde in PBS for 20 minutes at room temperature, washed three times with PBS, and then treated with 5 mM Fura-2 AM for 45 minutes at room temperature in the dark. The slides were washed three times with PBS and examined using confocal microscopy (Zeiss LSM510 Meta).

Immunofluorescence analysis
HEK293 reporter cells were cultured on glass chamber slides and then treated with DMSO or A23187 for 15 hour. After treatment, the cells were washed with PBS, fixed with 4% formaldehyde, permeabilized in 0.3% Triton X-100, and blocked in 4% bovine serum albumin for 1 hour. The cells were stained with anti-PKCa antibody and then analyzed by confocal microscopy using a Zeiss LSM510 Meta microscope.