Receptors for basement membrane (BM) proteins, including dystroglycan (DG), coordinate tissue development and function by mechanisms that are only partially defined. To further elucidate these mechanisms, we generated a conditional knockout of DG in the epithelial compartment of the mouse mammary gland. Deletion of DG caused an inhibition of mammary epithelial outgrowth and a failure of lactation. Surprisingly, loss of DG in vivo did not disrupt normal tissue architecture or BM formation, even though cultured Dag1-null epithelial cells failed to assemble laminin-111 at the cell surface. The absence of DG was, however, associated with a marked loss in activity of signal transducer and activator of transcription 5 (STAT5). Loss of DG perturbed STAT5 signaling induced by either prolactin or growth hormone. We found that DG regulates signaling by both hormones in a manner that is dependent on laminin-111 binding, but independent of the DG cytoplasmic domain, suggesting that it acts via a co-receptor mechanism reliant on DG-mediated laminin assembly. These results demonstrate a requirement for DG in the growth and function of a mammalian epithelial tissue in vivo. Moreover, we reveal a selective role for DG in the control of multiple STAT5-dependent hormone signaling pathways, with implications for numerous diseases in which DG function is compromised.
Basement membranes (BMs) are formed adjacent to a wide range of cell types and exhibit diverse functions including the creation of physical barriers between tissue compartments, the provision of structural support to cells and tissues, and the provision of signaling cues for cellular orientation, migration, survival, proliferation and tissue-specific gene expression (Miner and Yurchenco, 2004; Yurchenco et al., 2004). Cell interactions with the BM are mediated by a varied set of receptors that include the β1 and β4 integrins, discoidin domain receptors, syndecans, sulfated glycolipids, Lutheran/BCAM and dystroglycan (Yurchenco and Patton, 2009). However, the distinct and cooperative roles of these receptors remain to be clearly elucidated.
Dystroglycan (DG) functions at virtually all cell–BM interfaces, including those of muscle and epithelial cells, and is present within the peripheral and central nervous systems (Durbeej et al., 1998; Moore et al., 2002; Nishimune et al., 2008). The extracellular domain of DG binds to prominent extracellular matrix proteins, including laminins, perlecan and agrin through O-linked carbohydrate modifications in the α-DG mucin domain (Barresi and Campbell, 2006; Ervasti and Campbell, 1993; Yoshida-Moriguchi et al., 2010). As a nearly ubiquitous extracellular matrix receptor, DG has the potential to have an important role in many developmental processes, and disruption of DG function might contribute to the progression of a diverse range of human diseases.
DG is best known as a central component of the dystrophin–glycoprotein complex (DGC), a multimeric protein complex of the muscle sarcolemma (Durbeej and Campbell, 2002). Genetic loss or depletion of DGC components or DGC-associated proteins is responsible for a large percentage of muscular dystrophies, and DG function itself is ablated or diminished in many of these diseases (Barresi and Campbell, 2006; Cohn and Campbell, 2000). Although genetic defects of the DG coding sequence have not yet been observed in human disease, defects of DG post-translational processing and function have been detected, leading to various muscular dystrophies with associated neurological defects (Barresi and Campbell, 2006). These diseases are rooted in a loss of DG ligand-binding properties in the extracellular domain, resulting from altered carbohydrate modifications, and collectively, have been labeled ‘dystroglycanopathies’ (Muntoni et al., 2008). Defects of DG glycosylation and function resembling those observed in dystroglycanopathies are evident in the progression of many carcinomas (Beltran-Valero de Bernabe et al., 2009; Singh et al., 2004) and appear to contribute to carcinoma progression through altered signaling between cells and the BM (Bao et al., 2009; Beltran-Valero de Bernabe et al., 2009; Muschler et al., 2002; Sgambato et al., 2003).
Conditional and chimeric gene-knockout studies have confirmed that loss of DG expression can recapitulate human disease phenotypes in numerous tissues (Cohn et al., 2002; Cote et al., 1999; Moore et al., 2002; Satz et al., 2008). These studies have established that DG functions in the stabilization of the muscle sarcolemma (Han et al., 2009), basement membrane integrity (Moore et al., 2002), neuromuscular junction maturation (Nishimune et al., 2008) and morphogenesis of the brain and eye (Moore et al., 2002; Satz et al., 2008); however, the precise mechanisms by which DG operates remain unclear.
Much less is known about DG function in the context of mammalian epithelial cell biology and development. Manipulation of DG in mammalian cell and organ culture supports diverse roles for DG, including roles in epithelial cell survival signaling (Li et al., 2002), branching morphogenesis (Durbeej et al., 2001), epithelial polarization (Muschler et al., 2002; Weir et al., 2006), and tissue-specific gene expression (Weir et al., 2006; Xu et al., 2009). Additionally, DG is crucial for the high-affinity assembly of laminin on the cell surface of cultured mammary epithelial and embryonic stem cells (Henry et al., 2001b; Weir et al., 2006). However, the importance of DG to mammalian epithelial tissue development in vivo is not clear. The conditional deletion of DG from the luminal epithelium of the prostate gland did not produce obvious defects of tissue development or function, but a deletion from the myoepithelial (basal epithelial) compartment of the prostate was not assessed (Esser et al., 2010). As yet, the conditional deletion of Dag1 gene expression has not been reported for any other adult mammalian epithelial lineage.
Here, we have generated the conditional knockout of DG in the entire epithelial compartment of the mouse mammary gland, including both basal and luminal epithelial cells, and assessed the role of DG in the morphogenesis and function of this epithelial tissue in vivo. These results demonstrate a role for DG in mammary gland outgrowth and function, providing a clear demonstration of tissue defects resulting from the loss of DG within an epithelial cell lineage of adult transgenic mice. Importantly, these results reveal a selective and potent role for DG in the modulation of STAT5 signaling in vivo, with implications for signaling through several STAT5-dependent pathways.
Deletion of Dag1 gene expression by K14-Cre activity arises in both luminal and basal epithelial cells early in mammary gland development
The conditional knockout of Dag1 gene expression from the epithelial compartment of the mouse mammary gland was achieved by Cre-loxP recombination, crossing the floxed-DG transgenic mouse line (Cohn et al., 2002; Moore et al., 2002) with the K14-Cre line expressing the Cre transgene from the keratin-14 (K14) promoter (Dassule et al., 2000). The K14-Cre transgene has induced DNA recombination that is ultimately detected in both luminal and myoepithelial cell populations of the adult mammary gland, suggesting earlier expression in a common progenitor cell population (Jonkers et al., 2001) but the precise pattern of K14-Cre expression in early mammary gland development has not yet been established. Crossing the K14-Cre transgenic line with the Rosa26R reporter mouse line (Soriano, 1999) revealed K14-Cre-mediated DNA recombination in the mammary buds at day embryonic day 14 (E14) of development (Fig. 1A), coinciding with the earliest invasion of the nascent gland into the mesenchyme (Robinson, 2007). This activity might be initiated just before broader activation of K14-Cre expression in the epidermis at E13.5 (Jonkers et al., 2001) (supplementary material Fig. S1). Cre-induced genetic alteration was perpetuated throughout the developing gland at birth (Fig. 1B) and in the vast majority of mammary epithelial cells, both myoepithelial and luminal, in glands from nulliparous, mid-pregnant and lactating animals (Fig. 1C,D and data not shown).
Mice bearing the floxed Dag1 locus (DGfl/wt or DGfl/fl) were crossed with bi-transgenic mice bearing the floxed DG locus and K14-Cre transgene. The Rosa26R transgene was included in the background of these crosses to permit the visualization of cells that had undergone DNA recombination in Cre-expressing mice. The knockout genotype (DGfl/fl; K14-Cre+), abbreviated to ΔDGK14-Cre, was obtained in ratios consistent with mendelian inheritance. ΔDGK14-Cre animals were viable as adults, had comparable body weight to control animals and were fertile. Rosa26R reporter activity was evident throughout the epithelium in mammary glands of ΔDGK14-Cre mice (supplementary material Fig. S2). Immunostaining of control tissues, lacking Cre expression, and ΔDGK14-Cre mammary tissues confirmed the loss of DG expression (Fig. 2A). In addition, immunoblotting of primary epithelial cultures from control and ΔDGK14-Cre mammary glands revealed the virtually complete loss of DG expression from the Cre-expressing glands (Fig. 2B).
Loss of DG perturbs epithelial outgrowth and mammary gland function
Mammary gland outgrowth and development was first assessed by whole mount analysis of the fourth (inguinal) gland in nulliparous female mice at 8 weeks of age. In control mice, mammary epithelial outgrowth extended well beyond the central lymph node (Fig. 3A). Comparable outgrowth was observed in heterozygous DGfl/wt;K14-Cre mice (bearing the K14-Cre and Rosa26R transgenes and DGfl/wt genotype) (Fig. 3B). By contrast, outgrowth of the gland in ΔDGK14-Cre mice was impeded, barely surpassing the central lymph node of the inguinal gland (Fig. 3C,D). Despite the impeded outgrowth, epithelial branching occurred and terminal end bud formation was not perturbed.
Lactation is enabled by a dramatic outgrowth of the epithelia during pregnancy, and by lobuloalveolar differentiation. To assess development of DG-knockout mammary glands during pregnancy and lactation, mammary glands were harvested from adult female mice at midpregnancy [day 14.5 post conception (d14.5)] and at day 1 of lactation (L1). At mid-pregnancy, control heterozygous, DGfl/wt;K14-Cre mice exhibited normal outgrowth and development. The glands of mid-pregnant ΔDGK14-Cre mice (Fig. 4A) were not discernibly different from control mice. At L1, pups of control and heterozygous, DGfl/wt;K14-Cre mice were viable; lactation was evident by the appearance of a milk spot in the stomach of pups (supplementary material Fig. S3A) and by their normal growth and maturation. However, in ΔDGK14-Cre mice, the majority of mothers demonstrated a failure of lactation, with pups dying within 1 day of birth. Despite active nursing, the pups appeared emaciated and a milk spot did not appear (supplementary material Fig. S3B), indicating that the mothers failed to produce sufficient quantities of milk. Of 29 ΔDGK14-Cre females giving birth, 15 (52%) failed at the stage of lactation. Of these 15 mothers, 12 failed to support the survival of any pups, and three were able to support through maturation only two or three pups from initial litters of six or more. The defect of lactation was not reversed by subsequent pregnancies; mice that failed in lactation with first litters also failed with subsequent litters.
Whole-mount analysis revealed that the growth of mammary glands in ΔDGK14-Cre mothers at the L1 stage had not progressed significantly beyond that seen at d14.5 of mid-pregnancy (Fig. 4B and supplementary material Fig. S4). This contrasted with control tissues, which displayed extensive growth and expansion of the lobuloalveolar units. Quantification of L1 mammary gland whole mounts demonstrates a 20% decrease in gland growth (Fig. 4C). Despite the impeded outgrowth of the gland, expression of milk protein genes was not disrupted in lactating ΔDGK14-Cre mice (supplementary material Fig. S5).
DG loss disrupts laminin assembly at the epithelial cell surface but does not perturb epithelial cytoarchitecture or basement membrane formation in vivo
DG has been implicated in cell-surface laminin assembly and polarity signaling in assays using immortalized mammary epithelial cells (Muschler et al., 2002; Weir et al., 2006). In primary cultures, laminin assembled extensively at the surface of mammary epithelial cells from control animals (Fig. 5A), and similar assembly was observed in immortalized mammary epithelial cell lines (Fig. 5B). In cultures from ΔDGK14-Cre animals, significant laminin assembly was absent (Fig. 5A), replicating results obtained in immortalized cell lines lacking DG expression (Fig. 5B) (Weir et al., 2006). Quantification in primary cultures of control and ΔDGK14-Cre mammary epithelial cells revealed greater than 80% reduction in the assembly of laminin in DG-knockout cells (Fig. 5C). Surprisingly, despite this defect observed in primary cultures, mammary glands from ΔDGK14-Cre animals did not exhibit changes in tissue cytoarchitecture or basement membrane formation in vivo. Immunostaining for epithelial polarity markers in tissue sections from ΔDGK14-Cre mammary glands showed normal distribution of the tight junction protein ZO-1 at the apical surface and α6 integrin at the basal surface (supplementary material Fig. S6A). Immunostaining with myoepithelial markers showed a comparable distribution of luminal and myoepithelial cells in the tissues of control and ΔDGK14-Cre mice, indicating no significant changes to the myoepithelial lineage (supplementary material Fig. S6B). Epithelial compartments lacking DG expression were surrounded by a continuous basement membrane, as evidenced by laminin staining, which appeared to be of normal integrity and thickness (Fig. 2 and supplementary material Fig. S6C).
DG is crucial for STAT5 activity in vivo
Outgrowth and function of the mammary gland is strongly influenced by growth hormone and prolactin signaling (Kelly et al., 2002), and by signaling from extracellular matrix receptors of the β1 integrin family (Li et al., 2005; Naylor et al., 2005). A shared downstream target of these extracellular signals is the activation of the transcription factor signal transducer and activator of transcription 5 (STAT5) (Cui et al., 2004; Hennighausen and Robinson, 2008; Naylor et al., 2005). Deletion of STAT5 expression in the mammary gland causes inhibited outgrowth and function that resembles the phenotype we observed in the ΔDGK14-Cre mammary gland, although it is more severe in the STAT5-knockout animals (Cui et al., 2004; Liu et al., 1997). To test whether STAT5 functions were perturbed in the absence of DG in vivo, STAT5 activation was assayed in L1 stage mammary glands of control and ΔDGK14-Cre mice by immunostaining for its nuclear localization using an anti-STAT5 antibody, and by immunostaining directly for active STAT5 using an anti-phospho-STAT5 antibody. Immunostaining for total STAT5 in lactating tissues showed the presence of STAT5 in the mammary epithelial cells from both control and ΔDGK14-Cre mice; however, there was a clear exclusion of STAT5 from the nucleus in the epithelial cells of ΔDGK14-Cre mice, which indicated a decrease in STAT5 activation (Fig. 6A). Immunostaining specifically for phosphorylated STAT5 (P-STAT5) revealed an even more striking difference. In control tissues, P-STAT5 was clearly evident and enriched in the nuclei of luminal epithelial cells (Fig. 6B). In the glands of ΔDGK14-Cre mice, P-STAT5 staining was very weak, confirming a dramatic loss of STAT5 activity in the absence of DG. Loss of nuclear STAT5 and P-STAT5 staining was also observed in 8-week-old nulliparous mice, coinciding with the impeded outgrowth observed at this stage (supplementary material Fig. S7).
DG modulates growth-hormone-induced STAT5 activity
STAT5 mediates signaling from several hormones and cytokines, which, in the mammary gland, include both prolactin and growth hormone. Disruption of prolactin receptor signaling can explain the defect of outgrowth during pregnancy, but not the observed defect of outgrowth in nulliparous females (Oakes et al., 2008). If DG acts at the level of STAT5 activation, then loss of STAT5 activation in the ΔDGK14-Cre mammary gland could also obstruct signaling through the growth hormone receptor, contributing to defects of outgrowth in both nulliparous and pregnant animals. This was tested using paired, DG-knockout and DG-expressing mammary epithelial cells that were established previously from DGfl/fl mice (MEpL cells) (Weir et al., 2006). To activate both DG and STAT5 signaling pathways, prolactin or growth hormone were added to these cells cultured in three-dimensional (3D) gels of reconstituted basement membrane (rBM) proteins. Immunostaining for P-STAT5 in the cell nuclei showed clear STAT5 activation in the DG-expressing cells following 24 hours exposure to either prolactin or growth hormone; but STAT5 activation was attenuated in DG-knockout cells (Fig. 7A). The levels of STAT5 activation were quantified by immunoblotting for P-STAT5 and total STAT5 in each cell population (Fig. 7B). This revealed that sustained activation of STAT5 was significantly diminished in DG-knockout cells; reduced by 62% for prolactin and 47% for growth hormone (Fig. 7C). Quantitative PCR (qPCR) measurements of β-casein and whey acidic protein (WAP) gene expression, which are both induced by STAT5 activation in mammary epithelial cells, also revealed a strong reduction in signaling induced either by prolactin (reduced by 81% and 90%, respectively) or growth hormone (reduced by 25% and 65%, respectively) in DG-knockout cells (Fig. 7D,E), mirroring the direct assays of activated STAT5. Similarly, induction of IGF-1 expression by prolactin or growth hormone signaling was very strongly attenuated in DG-knockout cells (by 96% in both cases) (Fig. 7F). These data demonstrate by several measures that DG modulates STAT5 activity induced either by prolactin or growth hormone receptor signaling in a cell autonomous manner.
The modulation of prolactin receptor signaling by the extracellular matrix, specifically laminin-111, has previously been established in cultured mammary epithelial cells (Streuli et al., 1995). The relationship of DG to growth hormone signaling presented above suggests these same relationships established for prolactin receptor signaling will also hold true for growth hormone receptor signaling. To test the dependence of growth hormone receptor signaling on laminin-111, MEpL cells expressing wild-type DG were plated on plastic and overlayed with rBM proteins or purified laminin-111, and stimulated with growth hormone for 72 hours. Immunoblotting extracts from these cells revealed a greater than twofold increase in the induction of the milk protein β-casein in the presence of rBM proteins or purified laminin when compared with GH treatment alone (Fig. 8A). Additionally, qPCR analysis of Igf1 mRNA induction by GH also revealed a greater than twofold increase in Igf1 expression (Fig. 8B), again confirming modulation by laminin-111.
Previously, we demonstrated that DG can mediate prolactin signaling in a manner that is independent of the DG cytoplasmic domain, through laminin-111 binding and assembly in an apparent co-receptor relationship with β1 integrins (Weir et al., 2006). To test whether the cytoplasmic domain of DG is necessary for the modulation of growth hormone signaling, MEpL cells expressing wild-type DG or DG with the entire cytoplasmic domain deleted (ΔC; residues 780–895) were grown on 3D gels of rBM proteins and stimulated with prolactin or growth hormone for 24 hours. Analysis of activated STAT5 signaling with an anti-P-STAT5 antibody revealed that MEpL cells expressing the ΔC DG construct modulated both prolactin and growth hormone receptor signaling as efficiently as was observed with wild-type DG (Fig. 9A,B). Immunoblotting extracts from these cells revealed greater than 6.5-fold induction of β-casein with prolactin and fivefold more with growth hormone (β-casein to E-cadherin ratios of wild-type and ΔC versus knockout MEpL cells) when comparing the wild type or ΔC MEpL cells with DG-knockout cells (Fig. 9A). Additionally, growth hormone induction of Igf1 transcript through the ΔC DG construct was as efficient as wild-type DG (Fig. 9C) as determined by qPCR. These data demonstrate that laminin-induced regulation of hormone signaling requires the extracellular but not cytoplasmic domain of dystroglycan.
The multifaceted functions of given proteins are often exposed by exploring their activities in distinct, tissue-specific contexts. We report here the direct assessment of DG knockout in an adult mammalian epithelial tissue, revealing a vital role for DG in the growth and function of the mammary gland. Among the many functions attributed to DG from cell and organ cultured studies, this in vivo work asserts a clear and important role for DG in the control of STAT5 signaling. STAT5 activity was markedly reduced in mammary epithelial cells lacking DG. Loss of STAT5 activation was evidenced by nuclear exclusion of total STAT5, and by loss of P-STAT5 signals in immunostained tissue sections, and was evident at all stages of adult mammary gland development. Moreover, mammary glands of ΔDGK14-Cre mice exhibited a reduced outgrowth during pregnancy, resulting in a failure of mothers to adequately nourish their pups. The phenotype appears to be caused by reduced outgrowth because there is no evidence of premature involution, as measured by changes in STAT3 activity (supplementary material Fig. S8) (Philp et al., 1996). This defect of mammary gland outgrowth and function in the ΔDGK14-Cre mice resembles a milder form of the phenotypes obtained with the mammary-gland-specific knockouts of STAT5a, STATa/b and the STAT5 activator JAK2 (Cui et al., 2004; Liu et al., 1997; Wagner et al., 2004). A milder phenotype than that observed in STAT5-knockout mice would be anticipated in the ΔDGK14-Cre mice because STAT5 activity is reduced but not eliminated, as observed in cultured DG-knockout cells (Figs 7 and 9). This convergence of phenotypes in the DG, STAT5 and JAK2 knockouts is, therefore, evidence of a selective and important role for DG in the modulation of STAT5 activity.
K14-Cre-mediated knockout of DG modifies both myoepithelial and luminal cells of the mammary gland, therefore, DG loss in myoepithelial cells might also contribute to the observed phenotype. For example, loss of oxytocin signaling in myoepithelial cells can also lead to a decrease in STAT5 activation at L1, failed lactation and a decrease in post partum mammary gland development at L3 (Wagner et al., 1997). We cannot rule out possible effects on oxytocin signaling in the failed lactation. However, disruption of oxytocin signaling would not appear to explain the loss of epithelial outgrowth observed in ΔDGK14-Cre mice at 8 weeks of age or at the L1 stage, and does not explain the cell-autonomous modulation of STAT5 activity by DG that was observed in cell culture. All the same, DG function is known to stabilize the muscle sarcolemma (Han et al., 2009), and the potential exists for important functions of DG in the contractile myoepithelial cell population.
Laminin-111 signaling is known to regulate prolactin receptor signaling in cultured mammary epithelial cells (Streuli et al., 1995) and has been shown recently to act by maintaining sustained STAT5 activation (Xu et al., 2009). The results we present here provide in vivo evidence that DG participates in the pathway between laminin binding at the cell surface and the sustained activation of STAT5. Importantly, we also demonstrate that the influence of basement membranes on STAT5 activation is not confined to prolactin-mediated signals, but can now be expanded to growth hormone signaling and probably that of other hormones and cytokines that signal through STAT5. To our knowledge, DG and cell interactions with the basement membrane have never before been implicated in the control of growth hormone signaling. However, this relationship has been presaged by previous animal studies where a strong inhibition of postnatal growth is evident in mice lacking DG functions (Lee et al., 2005; Levedakou et al., 2005; Satz et al., 2008) or laminin-α2 expression (Miyagoe et al., 1997). Thus far, this inhibition of postnatal growth has lacked an adequate molecular explanation, but it mirrors the phenotype obtained from the loss of STAT5 activity and growth hormone signaling in transgenic mice (Klover and Hennighausen, 2007).
The mechanism by which DG initiates signals from laminins is uncertain, but evidence increasingly points to a co-receptor mechanism and not a direct signaling function (Weir et al., 2006; Yurchenco et al., 2004). This conclusion is based largely on our demonstration that the cytoplasmic domain of DG is not essential to DG-mediated control of prolactin and growth hormone signaling; thus suggesting a model whereby DG operates as a co-receptor through anchorage and assembly of laminin at the cell surface (Weir et al., 2006). The role of DG in cell-surface laminin-111 assembly was demonstrated previously using embryonic stem cells and immortalized mammary epithelial cells (Henry and Campbell, 1998; Weir et al., 2006), and confirmed again here using primary mammary epithelial cultures. The precise mechanism of this proposed co-receptor function remains to be elucidated. DG might alter laminin conformation or, alternatively, DG-mediated laminin assembly might alter the mechanical properties of the basement membrane and modify mechanosignal transduction via co-receptors. In either case, it is evident from our results that the presence of the BM alone is not sufficient for proper STAT5 regulation, but appears to rely instead on a specific organization of BM components, conferred by dystroglycan.
The β1 integrin receptor family has also been implicated in laminin signaling in mammary epithelial cells (Muschler et al., 1999; Naylor et al., 2005; Streuli et al., 1995), and is a leading candidate for a signaling co-receptor for DG in the laminin-STAT5 regulatory axis. According to the DG–integrin co-receptor model, DG functions in the same pathway as laminin-binding β1 integrins. In this light, it is significant that the DG knockout phenotype in the mammary gland mimics the knockout phenotypes observed for β1 integrins, and the integrin-associated kinases integrin-linked kinase (ILK) and focal adhesion kinase (FAK). Similarly to the DG knockout, β1 integrin, ILK and FAK knockouts each cause a failure of epithelial outgrowth and function in the mammary gland, and each perturbs STAT5 activity (Akhtar et al., 2009; Nagy et al., 2007; Naylor et al., 2005), providing genetic support in vivo for the hypothesized co-receptor relationship between DG and β1 integrin.
The β1 integrins and DG are nearly ubiquitous extracellular matrix receptors, and are co-expressed in all cell types exposed to laminins. Therefore, the proposed co-receptor relationship between DG and β1 integrins in laminin signaling might operate in many different tissues. Similarly, STAT5 is the most widely expressed of the STAT proteins, with known functions in epithelia, muscle tissues, liver, the immune system and the central nervous system (Hennighausen and Robinson, 2008). Observation of the potent influence of laminin, DG and integrins on the regulation of STAT5 activity in the mammary gland suggests that this co-receptor-initiated pathway of STAT5 regulation could be operational in many normal developmental processes. The disruption of this pathway and loss of STAT5 activation might explain human disease manifestations, including unexplained pathologies of the eye and brain that accompany congenital muscular dystrophies. Notably, a close relationship between DG and integrin signaling has been observed in the brain where, similarly to our observations in the mammary gland, there is a convergence of phenotypes observed among the conditional knockouts of DG, β1 integrin, FAK and ILK, where each exhibits defects of cortical organization (Beggs et al., 2003; Graus-Porta et al., 2001; Moore et al., 2002; Niewmierzycka et al., 2005; Satz et al., 2008). Similar CNS abnormalities are observed in mouse models of congenital muscular dystrophies originating from defects in laminin-α2, α7β1 integrin or DG function (Lee et al., 2005; Levedakou et al., 2005; Miyagoe et al., 1997; Satz et al., 2008), and are reflected in human congenital muscular dystrophy patients with defects of laminin-α2 and DG (Reed, 2009). Correspondingly, mice expressing hypomorphic Stat5 alleles demonstrate defects of cell proliferation and neural migration in the CNS (Hennighausen and Robinson, 2008; Markham et al., 2007), portending that loss of STAT5 activity can underlie CNS defects of congenital muscular dystrophies.
Altered interactions between cells and the basement membrane are also strongly implicated in the progression of many cancers, including those of the breast (Bissell et al., 2002). Importantly, loss of DG function is evident in many carcinomas, including breast cancer (Henry et al., 2001a; Muschler et al., 2002) and in the progression of other cancer types, including gliomas and pediatric cancers (Calogero et al., 2006; Martin et al., 2007). Because DG is a mediator of normal cell and basement membrane interactions and is lost in tumor progression, it is hypothesized to possess tumor suppressor functions, which is supported by cell behavior assays in normal and cancerous cells (Bao et al., 2009; Beltran-Valero de Bernabe et al., 2009; Muschler et al., 2002; Sgambato et al., 2004; Weir et al., 2006). The role of DG in the regulation of STAT5 activity revealed here adds a new dimension to the potential role of DG in cancer progression through support of growth hormone and prolactin signaling, IGF-1 production and growth stimulation (Cotarla et al., 2004; Tan and Nevalainen, 2008).
Materials and Methods
Generation of mice with mammary-epithelial-specific knockout of dystroglycan
The floxed DG mouse line has been previously described (Moore et al., 2002). The transgenic mouse lines K14-Cre [Tg (KRT 14-cre) 1Amc/J, 004782] and Rosa26 [B6.129S4-Gt(Rosa)26Sortm1Sor/J, 003474] were obtained from The Jackson Laboratory (Bar Harbor, ME). All lines used in this study were backcrossed into the C57BL/6 background more than six times before analysis. Female mice of more than 8 weeks of age were crossed and glands were harvested at midpregnancy [day 14.5 post conception (d14.5)] and at day 1 of lactation (L1). Loss of DG expression in the epidermis via K14-Cre activity did not produce any blistering or overt skin disease, and there were no apparent adverse effects on the health or longevity of the animals (data not shown). All animal use was reviewed and approved by the Institutional Animal Care and Use Committee of the California Pacific Medical Center Research Institute.
Dissected glands were fixed for 30 minutes in 2% paraformaldehyde in PBS, 0.1% NP-40, washed in PBS, and stained with X-gal solution overnight at room temperature. Carmine Alum staining was performed following fixation in Carnoy's solution. Paraffin embedding, sectioning and staining with hematoxylin and eosin were performed by the Histopathology Reference Laboratory (Hercules, CA). Outgrowth of the mammary gland in nulliparous mice was quantified by measuring the distance from the central lymph node to the tip of the most distal epithelial structure. The percentage epithelial coverage at L1 was calculated from images of mammary gland whole mounts in supplementary material Fig. S4 using Metamorph software (Molecular Devices). The outside edge of the epithelium extending beyond the lymph node was traced and thresholded to include only epithelium. The thresholded area was then divided by the total area. The ratio in control samples was designated as 100% epithelial coverage.
Primary mammary epithelial cells from control or ΔDGK14-Cre midpregnant mice were obtained as previously described (Weir et al., 2006). DG-knockout, DG-expressing mammary epithelial cells (MEpL cells) and the cytoplasmic DG deletion (ΔC) were established previously from DGfl/fl mice (Weir et al., 2006) and grown in DME/F12, 2% FBS, 10 μg/ml insulin, 5 ng/ml EGF (BD Biosciences). For culture of rBM proteins on 3D gels, plastic plates were coated with a thin layer of Matrigel (BD Biosciences). The following day medium was replaced with new medium containing 1.5% Matrigel in suspension. After 1 week, the medium was replaced with serum-free medium without EGF but with 0.5 μg/ml hydrocortisone. The following day, either sheep prolactin (3 μg/ml) (Sigma) or mouse growth hormone (1 μg/ml) (National Hormone and Peptide Program, Torrance, CA) was added. 24 hours later, cells were harvested for assessment of STAT5 activation. For biochemistry, cells were isolated from Matrigel in cold PBS with 5 mM EDTA as described (Xu et al., 2009).
Biochemistry and SDS-PAGE
Cells were lysed in RIPA lysis buffer (50 mM Tris-HCl, pH 8.0, 1% NP-40, 0.5% deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA 1 mM PMSF, 50 mM NaF, 100 mM Na4P2O7, 10 mM Na β-glycerophosphate, 1 mM Na3VO4, 1× protease inhibitor cocktail-Chemicon) and quantified with the DC protein assay (Bio-Rad). 10 μg of extracted proteins were resolved on SDS-PAGE gels, transferred to PVDF membranes (Immobilon-P) (Millipore) and immunoblotted as described (Weir et al., 2006). The following primary antibodies were used for immunoblotting: 1:250 rabbit anti-P-STAT5 (Invitrogen), 1:500 rabbit anti-STAT5 (Santa Cruz Biotechnology), 1:5000 rabbit anti-actin (Sigma), 1:2000 mouse E-cadherin (BD Transduction Labs), 1:2000 mouse β-DG (MANDAG-2, Developmental Studies Hybridoma Bank, Iowa), 1:1000 mouse β1 integrin (PharMingen). HRP-conjugated secondary antibodies specific for rabbit and mouse IgG (Jackson ImmunoResearch) were used at 1:15,000. Immunoblot signals were visualized by enhanced chemiluminescence (Super Signal West Femto, Pierce, Rockford, IL) and digitally imaged with an Alpha Innotech imager (San Leandro, CA). Before image processing, bands were quantified using Alpha Ease EC analysis software (Alpha Innotech). Figures are inverted images processed with Adobe Photoshop.
Immunofluorescence microscopy of tissue sections
Dissected mammary gland tissue was embedded in OCT (Tissue-Tek) and frozen on dry ice. 10-μm-thick sections were cut with a cryostat, transferred to charged slides, and fixed in 100% methanol for 20 minutes at −20°C, washed with PBS, and blocked with 3% BSA, 2% goat serum, 0.2% Triton X-100 (Sigma) in PBS (blocking buffer) for 1 hour. Sections were then incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: 1:100 rabbit anti-ZO-1 (Zymed), 1:200 rabbit anti-laminin, 1:50 rabbit anti-P-STAT5 (Invitrogen), 1:50 rabbit anti-STAT5 (Santa Cruz Biotechnology), 1:1000 rabbit anti-keratin-14 (Sigma), 1:30 rat GoH3 mAb anti-α6-integrin (Chemicon), 1:50 mouse anti-β-DG (MANDAG-2, Developmental Studies Hybridoma Bank, Iowa), 1:400 mouse anti-α-smooth-muscle-actin (clone 1A4, Sigma), 1:200 mouse E-cadherin (BD Transduction Labs). Sections were washed three times with PBS, stained for 1 hour with the following secondary antibodies diluted in blocking buffer: 1:50 goat anti-rabbit-FITC, 1:200 goat anti-mouse-CY3 (Jackson ImmunoResearch) or 1:200 goat anti-rabbit-Rhodamine. Sections were then washed three times in PBS and mounted with Fluoromount G (Electron Microscopy Sciences) and a coverslip. Sections stained for P-STAT5 or STAT5 were counterstained with 10 μg/ml propidium iodide (Sigma) to stain the nuclei. For staining with anti-β-DG antibodies: after fixation sections were incubated in 0.1 M glycine, 6 M urea, pH 3.5 at 4°C for 1 hour followed by blocking and staining as above. Confocal images were acquired with a Nikon C1 laser scanning confocal attached to a Nikon TE2000 inverted microscope.
Immunofluorescence microscopy of cultured cells
After hormone treatment as described above, cells in Matrigel were gently lifted from plates and spread onto slides, fixed and blocked as above. Cells were incubated with 1:50 rabbit anti-P-STAT5 (Invitrogen) primary antibodies overnight at 4°C. Cells were then washed three times with PBS and incubated for 1 hour at room temperature with 1:50 goat anti-rabbit FITC (Jackson ImmunoResearch) diluted in blocking buffer. Cells were washed three times with PBS, stained with 10 μg/ml propidium iodide, mounted and imaged as above.
Fluoroscein-isothiocyanate (FITC)-labeled laminin-111 was prepared as described (Weir et al., 2006). Cells were grown overnight on Lab-Tek II glass chamber slides. FITC-laminin was added at a 10 ng/ml, incubated overnight, and treated cells were fixed with paraformaldehyde. Light and fluorescent microscopy was performed on a TE2000 Nikon inverted microscope with a Photometrics Coolsnap HQ CCD camera controlled with Nikon Elements software. Laminin assembly was quantified by applying the same threshold to the assembled fluorescent laminin images and dividing by the cell area using Metamorph image analysis software. The fluorescence intensity accumulated on DG-knockout cells was then normalized to that on DG-expressing cells to obtain the percentage of normal laminin assembly, where normal assembly=100%.
Real-time quantitative PCR
RT-PCR was performed as described (Xu et al., 2009) using the following primers: GAPDH: forward, 5′-CCCCTGGCCAAGGTCATCCATGAC-3′, reverse, 5′-CATACCAGGAAATGAGCTTGACAAAG-3′; β-Casein: forward, 5′-GCTCAGGCTCAAACCATCTC-3′, reverse, 5′-TGTGGAAGGAAGGGTGCTAC-3′; WAP: forward, 5′-AAAAGCCAGCCCCATTGAGG-3′, reverse, 5′-AGGGTTATCACTGGCACTGG-3′; IGF-1: forward, 5′-TCGTCTTCACACCTCTTCTACCT-3′, reverse, 5′-ACTCATCCACAATGCCTGTCT-3′; E-cadherin: forward, 5′-AATGGCGGCAATGCAATCCCAAGA-3′, reverse, 5′-TGCCACAGACCGATTGTGGAGATA-3′. The Lightcycler PCR protocol was 50°C for 2 minutes, 95°C for 10 minutes, 40 cycles of 95°C for 15 seconds and 60°C for 1 minute. Following amplification, melting curve analysis verified the presence of a single PCR product. Reactions were performed in triplicate and the Ct values normalized to GAPDH expression.
This work was supported by the Department of Defense Breast Cancer Research Program Grant W81XWH-07-1-0416 and National Institute of Health Grant 5R01CA109579. K.P.C. is an Investigator of the Howard Hughes Medical Institute. The authors wish to thank Shruti Nayak for technical assistance and Zena Werb, Armin Akhavan, Marisa Oppizzi, Aejaz Sayeed, and Shan Lu for helpful discussion. Deposited in PMC for release after 6 months.
Supplementary material available online at http://jcs.biologists.org/lookup/suppl/doi:10.1242/jcs.070680/-/DC1
- Accepted July 20, 2010.
- © 2010.