Table of Contents
IN THIS ISSUE
- LINCing the eukaryotic tree of life – towards a broad evolutionary comparison of nucleocytoplasmic bridging complexes
Summary: A broad evolutionary comparison of eukaryotic nucleocytoplasmic bridging complexes suggests that they might have evolved from protein complexes involved in attaching centrosomes to the nuclear envelope.
- Emerging roles of the single EF-hand Ca2+ sensor tescalcin in the regulation of gene expression, cell growth and differentiation
Summary: Tescalcin is a Ca2+-binding protein that regulates cell differentiation through interactions with NHE1, GSK3, the COP9 signalosome and some transcription factors. It is associated with cancer and developmental abnormalities.
- Human satellite-III non-coding RNAs modulate heat-shock-induced transcriptional repression
Highlighted Article: The satellite-III non-coding RNAs, expressed only during heat shock in human cells, recruit transcription factors to their nuclear foci, contributing to heat-shock-induced transcriptional repression.
- A wide reprogramming of histone H3 modifications during male meiosis I in rice is dependent on the Argonaute protein MEL1
Summary: Large-scale chromatin remodeling with dynamic alteration of H3K9 modifications occurred during male meiosis I in rice. This impacted on pivotal meiotic events and was dependent on the germline-specific Argonaute protein MEL1.
- TRIM17 contributes to autophagy of midbodies while actively sparing other targets from degradation
Highlighted Article: TRIM17 regulates selective autophagy by inhibiting the degradation of a range of substrates but promoting the autophagic removal of midbodies. Thus TRIM17 facilitates the precise discrimination between potential autophagy substrates.
- MEKK1-dependent phosphorylation of calponin-3 tunes cell contractility
Summary: Phosphorylation of calponin-3 by MEKK1, which is regulated by actomyosin contraction, leads to an increase in cell-generated traction stress, forming a new positive-feedback loop in the cellular contractile machinery.
- The polarity protein Scribble positions DLC3 at adherens junctions to regulate Rho signaling
Highlighted Article: A previously unrecognized PDZ ligand motif in the RhoGAP protein DLC3 is shown to direct the protein to adherens junctions through interaction with the polarity protein Scribble, where it locally regulates RhoA.
- An unrecognized function for COPII components in recruiting the viral replication protein BMV 1a to the perinuclear ER
Summary: Erv14 interacts with brome mosaic virus protein 1a, whose perinuclear ER localization requires Erv14 and the COPII coat components Sec24, Sec13 and Sec31, in contrast to their canonical roles in protein ER exit.
- The microtubule-binding and coiled-coil domains of Kid are required to turn off the polar ejection force at anaphase
Highlighted Article: Both, the microtubule-binding domain of Kid, which is activated by dephosphorylation of Thr463 at anaphase onset, and its coiled-coil domain inhibit the generation of polar ejection force, thereby facilitating chromosome segregation.
- ARHGEF10 directs the localization of Rab8 to Rab6-positive executive vesicles
Summary: An ARHGEF10 localization analysis using a novel in-house antibody revealed ARHGEF10 localization on vesicles emerging from the Golgi apparatus. A further proposal suggests that ARHGEF10 function is related to Rab8 localization on these vesicles and cell invasiveness.
- PrP-containing aggresomes are cytosolic components of an ER quality control mechanism
Summary: Prion protein (PrP)-containing cytosolic aggresomes are components of a quality-control mechanism of the ER. PrP molecules that fail to enter the ER will not be deposited in pre-existing aggresomes.
- Haspin kinase regulates microtubule-organizing center clustering and stability through Aurora kinase C in mouse oocytes
Summary: Haspin kinase is a regulator of meiotic bipolar spindle assembly, which is mediated by acentriolar microtubule-organizing centers (MTOCs), and acts by regulating the localization of AURKC to the MTOCs in mouse oocytes.
- Talin2-mediated traction force drives matrix degradation and cell invasion
Summary: Talin2, previously presumed to function redundantly with talin1, binds to β integrins more strongly than talin1, and generates traction force to regulate invadopodium formation and cell invasion.
- Gα12 is required for renal cystogenesis induced by Pkd1 inactivation
Summary: Deletion of the gene encoding the G-protein subunit Gα12 prevents mouse kidneys from developing the kidney cysts induced by Pkd1 inactivation, which indicates that Gα12 signaling is crucial for renal cystogenesis.
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