Meiotic oocytes lack classic centrosomes and, therefore, bipolar spindle assembly depends on clustering of acentriolar microtubule-organizing centers (MTOCs) into two poles. However, the molecular mechanism regulating MTOC assembly into two poles is not fully understood. The kinase haspin (also known as GSG2) is required to regulate Aurora kinase C (AURKC) localization at chromosomes during meiosis I. Here, we show that inhibition of haspin perturbed MTOC clustering into two poles and the stability of the clustered MTOCs. Furthermore, we show that AURKC localizes to MTOCs in mouse oocytes. Inhibition of haspin perturbed the localization of AURKC at MTOCs, and overexpression of AURKC rescued the MTOC-clustering defects in haspin-inhibited oocytes. Taken together, our data uncover a role for haspin as a regulator of bipolar spindle assembly by regulating AURKC function at acentriolar MTOCs in oocytes.
The authors declare no competing or financial interests.
A.Z.B., A.L.N., A.S.G., S.M.Q., and D.D. performed and analyzed experiments. A.Z.B., P.S. and K.S. analyzed data and wrote the manuscript. All authors edited the manuscript.
This work was supported by grants from the National Institutes of Health (NIH) to K.S. [grant numbers R00 HD061657 and R01 GM112801]. S.M.Q. was supported by an NIH fellowship [grant number K12-GM093854]. D.D. and P.S. were supported by the National Sustainability Programme of the Ministerstvo Školství, Mládeže a Tělovýchovy (Czech Ministry of Education, Youth and Sports) [project number LO1609]. Deposited in PMC for release after 12 months.
Supplementary information available online at http://jcs.biologists.org/lookup/doi/10.1242/jcs.189340.supplemental
- Received March 14, 2016.
- Accepted August 17, 2016.
- © 2016. Published by The Company of Biologists Ltd