Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Workshops and Meetings
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Alerts
  • Contact
    • Contact JCS
    • Subscriptions
    • Advertising
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in

Search

  • Advanced search
Journal of Cell Science
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Journal of Cell Science

  • Log in
Advanced search

RSS   Twitter  Facebook   YouTube  

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Workshops and Meetings
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Alerts
  • Contact
    • Contact JCS
    • Subscriptions
    • Advertising
    • Feedback
In This Issue
PTP-PEST in Eph signalling
J Cell Sci 2016 129: e0203
  • Article
  • Info & metrics
Loading

Embedded Image

Ephrin receptor (Eph)–ephrin signalling is important for cell positioning and tissue patterning during development and in oncogenic contexts, controlling cell morphology and cell–cell adhesion through cytoskeletal remodelling. PTP-PEST, a ubiquitous protein tyrosine phosphatase that interacts with focal adhesion proteins and coordinates cell movement, has been shown to be recruited to EphA3-signalling complexes, but its role in the Eph-mediated effects is unclear. On page 277, Mariam Mansour, Eva Nievergall and colleagues thus thoroughly investigate the role of PTP-PEST in EphA3 function using cell fractionation to isolate cell-surface Eph-signalling clusters, which contain key focal adhesion and cytoskeletal proteins. They show that an N-terminal fragment of PTP-PEST, which is generated by localised caspase-3-mediated cleavage in response to ephrin-A5, is recruited to EphA3 clusters and controls EphA3 activation by directly affecting its phosphorylation status. This inhibits receptor internalisation and cell retraction. Interestingly, inhibiting actin cytoskeletal dynamics reduces the levels of EphA3 phosphorylation, similar to the effect of PTP-PEST overexpression, suggesting that the cytoskeletal reorganisation is not only a consequence of Eph activation but is also required for Eph clustering and activation. As EphA3 and PTP-PEST are known to promote tumour formation, the role of PTP-PEST in controlling Eph-induced cytoskeletal remodelling described here also has important implications for our understanding of cancer cell motility.

  • © 2016. Published by The Company of Biologists Ltd
Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

Email

Thank you for your interest in spreading the word on Journal of Cell Science.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
PTP-PEST in Eph signalling
(Your Name) has sent you a message from Journal of Cell Science
(Your Name) thought you would like to see the Journal of Cell Science web site.
Share
PTP-PEST in Eph signalling
J Cell Sci 2016 129: e0203
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
PTP-PEST in Eph signalling
J Cell Sci 2016 129: e0203

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
  • Info & metrics

Related articles

Cited by...

More in this TOC section

  • Nuclear dynamics of intranuclear positioning of sea urchin gene loci
  • Role for α-tubulin PTMs in sensory dendrite development
  • Visualising mitochondrial translation in situ
Show more In This Issue

Similar articles

Other journals from The Company of Biologists

Development

Journal of Experimental Biology

Disease Models & Mechanisms

Biology Open

Advertisement

Call for papers – Reconstituting cell biology

Deadline for submissions: 1 May 2018

We are now accepting submissions for our upcoming special issue on ‘Reconstituting cell biology’, guest edited by Manuel Théry. Click here for more information.


Opinion – The triple helix of collagens

In a new Opinion article, Billy G. Hudson and colleagues argue that the triple helix is an ancient protein structure of fundamental importance in building the extracellular matrix that enabled animal multicellularity and tissue evolution.


JCS Meeting – Cell dynamics: organelle-cytoskeleton interface

The second in our series of cell dynamics meetings now turns to organelles. This May 2019 meeting in Lisbon, Portugal, aims to bring together scientists studying the interface between organelles and the cytoskeleton at different scales and perspectives using a range of model systems. Find out more and register your interest here.


Review – Dynamics of cortical domains in early Drosophila development

Cortical domains are a central feature of epithelial and polarised cells. Anja Schmidt and Jörg Grosshans present a Review of the dynamics of cortical domains in early Drosophila embryos and discuss underlying mechanisms for formation of cortical domains.


Cell scientist to watch – 2018 Hooke medal winner Andrew McAinsh

“…identify an interesting, good scientific question and try to answer it.”

Andrew McAinsh, Head of Division of Biomedical Sciences at Warwick Medical School, is interested in understanding how the chromosomal multi-protein complex, the kinetochore, ensures error-free chromosome segregation. He is the recipient of the 2018 Hooke medal, established to recognize an emerging leader in cell biology.

We also feature interviews with first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Check out our recent First Person interviews with Thibault Courtheoux and Alghassimou Diallo, Francesco Consolato and Emily Herman.


Articles of interest in our sister journals

Head formation requires Dishevelled degradation that is mediated by March2 in concert with Dapper1
Hyeyoon Lee, Seong-Moon Cheong, Wonhee Han, Youngmu Koo, Saet-Byeol Jo, Gun-Sik Cho, Jae-Seong Yang, Sanguk Kim, Jin-Kwan Han. Development 2018 145: dev143107

PUF-8 facilitates homologous chromosome pairing by promoting proteasome activity during meiotic entry in C. elegans
Ganga Anil Kumar, Kuppuswamy Subramaniam. Development 2018 145: dev163949

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Interviews
  • Alerts

About us

  • About Journal of Cell Science
  • Editors and Board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Workshops and Meetings
  • The Company of Biologists

For Authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Figure preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • JCS Prize
  • Biology Open transfer

Journal Info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Alerts

Contact

  • Contact Journal of Cell Science
  • Subscriptions
  • Advertising
  • Feedback

Twitter   YouTube   LinkedIn

© 2018   The Company of Biologists Ltd   Registered Charity 277992