Table of Contents
IN THIS ISSUE
FIRST PERSON
CELL SCIENCE AT A GLANCE
- Targeting and translocation of proteins to the endoplasmic reticulum at a glance
Summary: Targeting and translocation into the ER are mediated by multiple pathways, which together enable the correct localization of about 30% of any eukaryotic proteome.
REVIEW
- The EGFR odyssey – from activation to destruction in space and time
Summary: We review the molecular events that occur during EGFR activation, its trafficking through the endosomal system and its subsequent downregulation.
RESEARCH ARTICLES
- Dynamic changes in the interchromosomal interaction of early histone gene loci during development of sea urchin
Highlighted Article: During the morula stage in sea urchin embryos, early histone gene loci undergo interchromosomal interactions that are dependent on the active transcriptional status.
- Functional analyses of the CIF1–CIF2 complex in trypanosomes identify the structural motifs required for cytokinesis
Summary: The trypanosome CIF1 zinc-finger motifs are essential for CIF1–CIF2 complex assembly, and along with the coiled-coil motifs in CIF1 are required for initiation of cytokinesis.
- Effects of mutating α-tubulin lysine 40 on sensory dendrite development
Highlighted Article: Neurons are enriched in post-translationally modified microtubules. Targeted mutagenesis of endogenous α-tubulin in flies reveals that dendrite branch refinement is altered by acetylation-blocking mutations.
- Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression
Summary: Knockout of giantin in a genome-engineered cell line and zebrafish models reveals the capacity of the Golgi to control its own biochemistry through changes in gene expression.
- Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast
Summary: The RNA-binding protein Zfs1 regulates accumulation of the G1 cyclin Puc1, a process that is modulated by phosphorylation. This regulatory mechanism has an important impact on cell differentiation.
- CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells
Summary: CREB3L1 has a role during the adaptation of the secretory pathway in response to high secretory demand in thyroid cells, a process with direct impact on thyroid physiology.
- RhoC regulates the actin remodeling required for phagosome formation during FcγR-mediated phagocytosis
Summary: RhoC accumulates at phagocytic cups and modulates an mDia1-dependent remodeling of cortical F-actin that is required for FcγR-mediated phagocytosis in macrophages.
- Quantitative analysis of multilayer organization of proteins and RNA in nuclear speckles at super resolution
Summary: Multi-color structured illumination microscopy imaging studies reveal a multilayer organization of nuclear speckles due to the interplay between favorable sequence-encoded intermolecular interactions of speckle-resident proteins and RNAs.
TOOLS AND RESOURCES
- In situ imaging of mitochondrial translation shows weak correlation with nucleoid DNA intensity and no suppression during mitosis
Highlighted Article: Imaging de novo mitochondrial protein synthesis with a novel fluorescence method shows the translational output throughout the cell and reveals a low correlation with mitochondrion genome copy number.
- ImaEdge – a platform for quantitative analysis of the spatiotemporal dynamics of cortical proteins during cell polarization
Summary: An open-source software package, ImaEdge, automates the segmentation of the cell cortex from time-lapse movies, and enables quantitative extraction of cortical protein dynamics in cells undergoing polarization.
- Real-time observation of light-controlled transcription in living cells
Summary: Blue light-induced chromatin recruitment (BLInCR) enables rapid and reversible targeting of effector proteins to nuclear loci with high temporal resolution for analyzing transcription activation.
This issue
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Other journals from The Company of Biologists
Call for papers – Reconstituting cell biology
Deadline for submissions: 1 May 2018
We are now accepting submissions for our upcoming special issue on ‘Reconstituting cell biology’, guest edited by Manuel Théry. Click here for more information.
Opinion – The triple helix of collagens
In a new Opinion article, Billy G. Hudson and colleagues argue that the triple helix is an ancient protein structure of fundamental importance in building the extracellular matrix that enabled animal multicellularity and tissue evolution.
JCS Meeting – Cell dynamics: organelle-cytoskeleton interface
The second in our series of cell dynamics meetings now turns to organelles. This May 2019 meeting in Lisbon, Portugal, aims to bring together scientists studying the interface between organelles and the cytoskeleton at different scales and perspectives using a range of model systems. Find out more and register your interest here.
Review – Dynamics of cortical domains in early Drosophila development
Cortical domains are a central feature of epithelial and polarised cells. Anja Schmidt and Jörg Grosshans present a Review of the dynamics of cortical domains in early Drosophila embryos and discuss underlying mechanisms for formation of cortical domains.
Cell scientist to watch – 2018 Hooke medal winner Andrew McAinsh
“…identify an interesting, good scientific question and try to answer it.”
Andrew McAinsh, Head of Division of Biomedical Sciences at Warwick Medical School, is interested in understanding how the chromosomal multi-protein complex, the kinetochore, ensures error-free chromosome segregation. He is the recipient of the 2018 Hooke medal, established to recognize an emerging leader in cell biology.
We also feature interviews with first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Check out our recent First Person interviews with Thibault Courtheoux and Alghassimou Diallo, Francesco Consolato and Emily Herman.
Articles of interest in our sister journals
Head formation requires Dishevelled degradation that is mediated by March2 in concert with Dapper1
Hyeyoon Lee, Seong-Moon Cheong, Wonhee Han, Youngmu Koo, Saet-Byeol Jo, Gun-Sik Cho, Jae-Seong Yang, Sanguk Kim, Jin-Kwan Han. Development 2018 145: dev143107
PUF-8 facilitates homologous chromosome pairing by promoting proteasome activity during meiotic entry in C. elegans
Ganga Anil Kumar, Kuppuswamy Subramaniam. Development 2018 145: dev163949