Table of Contents
IN THIS ISSUE
FIRST PERSON
CELL SCIENCE AT A GLANCE
- Targeting and translocation of proteins to the endoplasmic reticulum at a glance
Summary: Targeting and translocation into the ER are mediated by multiple pathways, which together enable the correct localization of about 30% of any eukaryotic proteome.
REVIEW
- The EGFR odyssey – from activation to destruction in space and time
Summary: We review the molecular events that occur during EGFR activation, its trafficking through the endosomal system and its subsequent downregulation.
RESEARCH ARTICLES
- Dynamic changes in the interchromosomal interaction of early histone gene loci during development of sea urchin
Highlighted Article: During the morula stage in sea urchin embryos, early histone gene loci undergo interchromosomal interactions that are dependent on the active transcriptional status.
- Functional analyses of the CIF1–CIF2 complex in trypanosomes identify the structural motifs required for cytokinesis
Summary: The trypanosome CIF1 zinc-finger motifs are essential for CIF1–CIF2 complex assembly, and along with the coiled-coil motifs in CIF1 are required for initiation of cytokinesis.
- Effects of mutating α-tubulin lysine 40 on sensory dendrite development
Highlighted Article: Neurons are enriched in post-translationally modified microtubules. Targeted mutagenesis of endogenous α-tubulin in flies reveals that dendrite branch refinement is altered by acetylation-blocking mutations.
- Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression
Summary: Knockout of giantin in a genome-engineered cell line and zebrafish models reveals the capacity of the Golgi to control its own biochemistry through changes in gene expression.
- Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast
Summary: The RNA-binding protein Zfs1 regulates accumulation of the G1 cyclin Puc1, a process that is modulated by phosphorylation. This regulatory mechanism has an important impact on cell differentiation.
- CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells
Summary: CREB3L1 has a role during the adaptation of the secretory pathway in response to high secretory demand in thyroid cells, a process with direct impact on thyroid physiology.
- RhoC regulates the actin remodeling required for phagosome formation during FcγR-mediated phagocytosis
Summary: RhoC accumulates at phagocytic cups and modulates an mDia1-dependent remodeling of cortical F-actin that is required for FcγR-mediated phagocytosis in macrophages.
- Quantitative analysis of multilayer organization of proteins and RNA in nuclear speckles at super resolution
Summary: Multi-color structured illumination microscopy imaging studies reveal a multilayer organization of nuclear speckles due to the interplay between favorable sequence-encoded intermolecular interactions of speckle-resident proteins and RNAs.
TOOLS AND RESOURCES
- In situ imaging of mitochondrial translation shows weak correlation with nucleoid DNA intensity and no suppression during mitosis
Highlighted Article: Imaging de novo mitochondrial protein synthesis with a novel fluorescence method shows the translational output throughout the cell and reveals a low correlation with mitochondrion genome copy number.
- ImaEdge – a platform for quantitative analysis of the spatiotemporal dynamics of cortical proteins during cell polarization
Summary: An open-source software package, ImaEdge, automates the segmentation of the cell cortex from time-lapse movies, and enables quantitative extraction of cortical protein dynamics in cells undergoing polarization.
- Real-time observation of light-controlled transcription in living cells
Summary: Blue light-induced chromatin recruitment (BLInCR) enables rapid and reversible targeting of effector proteins to nuclear loci with high temporal resolution for analyzing transcription activation.
This issue
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Cell scientist to watch – Serge Mostowy
“Focus on what you think is exciting; in turn, recognise and accept help when it is offered.”
Serge Mostowy heads a lab at the London School of Hygiene & Tropical Medicine, working on the molecular mechanisms underlying bacterial infection and the role of the cytoskeleton in cellular immunity. He shares the story behind his unexpected journey from a background in mathematics, through -omics, sequencing and microscopy to host–pathogen research, and how he has drawn inspiration at each stage from the scientists around him.
Catch up with early-career first authors in our First Person interviews. Recently, we spoke to Bhagawat C. Subramanian, Hannah Arbach, Sudeshna Nag and Patrick Cunningham.
Review – Where, how and why a cell eats itself to death
Shani Bialik, Santosh K. Dasari and Adi Kimchi review recent findings that demonstrate autophagy-dependent cell death in various contexts and begin to elucidate molecular mechanisms that render autophagy a lethal process.
Review – Failure is not an option
André Schneider and Torsten Ochsenreiter discuss recent discoveries regarding the unusual segregation system of the single-unit mitochondrial genome of T. brucei.
Meet the team
Meet the Journal of Cell Science team at conferences throughout October. Executive Editor Sharon Ahmad attends the ASMB meeting in Las Vegas and Reviews Editor Petra Gross will be at the EMBO workshop on ER function in health and disease in Lucca, Italy. Features & Reviews Editor Manuel Breuer will be at the FEBS 2018 Golgi meeting in Sorrento, Italy.
JCS Meeting – Cell dynamics: organelle-cytoskeleton interface
The second in our series of cell dynamics meetings now turns to organelles. This May 2019 meeting in Lisbon, Portugal, aims to bring together scientists studying the interface between organelles and the cytoskeleton at different scales and perspectives using a range of model systems. Find out more and apply for your place here.
Journal news – new fast-track decision process
Do you have a paper with reviews from another journal that you’d like to submit to Journal of Cell Science? We will fast-track your paper and give you an initial decision within a week. Find out more here.
preLights – Transient intracellular acidification regulates the core transcriptional heat shock response
Srivats Venkataramanan highlights a recent pre-print from Allan Drummond and colleagues, showing that a short-lived decrease in the pH of the cellular interior is critical to mount a response to environmental stresses such as increased temperature. This preLight features a response from first author Catherine Triandafillou, outlining the most exciting aspects of the project.
We are also pleased to announce that recent preLights selected for the cell science community will feature regularly as part of our issue Table of Contents.
Articles of interest in our sister journals
GILZ-dependent modulation of mTORC1 regulates spermatogonial maintenance
Hue M. La, Ai-Leen Chan, Julien M. D. Legrand, Fernando J. Rossello, Christina G. Gangemi, Antonella Papa, Qiang Cheng, Eric F. Morand, Robin M. Hobbs. Development 2018 145: dev165324
ROCK-nmMyoII, Notch and Neurog3 gene-dosage link epithelial morphogenesis with cell fate in the pancreatic endocrine-progenitor niche
Eric D. Bankaitis, Matthew E. Bechard, Guoqiang Gu, Mark A. Magnuson, Christopher V. E. Wright. Development 2018 145: dev162115