Table of Contents
IN THIS ISSUE
FIRST PERSON
CELL SCIENCE AT A GLANCE
- Targeting and translocation of proteins to the endoplasmic reticulum at a glance
Summary: Targeting and translocation into the ER are mediated by multiple pathways, which together enable the correct localization of about 30% of any eukaryotic proteome.
REVIEW
- The EGFR odyssey – from activation to destruction in space and time
Summary: We review the molecular events that occur during EGFR activation, its trafficking through the endosomal system and its subsequent downregulation.
RESEARCH ARTICLES
- Dynamic changes in the interchromosomal interaction of early histone gene loci during development of sea urchin
Highlighted Article: During the morula stage in sea urchin embryos, early histone gene loci undergo interchromosomal interactions that are dependent on the active transcriptional status.
- Functional analyses of the CIF1–CIF2 complex in trypanosomes identify the structural motifs required for cytokinesis
Summary: The trypanosome CIF1 zinc-finger motifs are essential for CIF1–CIF2 complex assembly, and along with the coiled-coil motifs in CIF1 are required for initiation of cytokinesis.
- Effects of mutating α-tubulin lysine 40 on sensory dendrite development
Highlighted Article: Neurons are enriched in post-translationally modified microtubules. Targeted mutagenesis of endogenous α-tubulin in flies reveals that dendrite branch refinement is altered by acetylation-blocking mutations.
- Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression
Summary: Knockout of giantin in a genome-engineered cell line and zebrafish models reveals the capacity of the Golgi to control its own biochemistry through changes in gene expression.
- Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast
Summary: The RNA-binding protein Zfs1 regulates accumulation of the G1 cyclin Puc1, a process that is modulated by phosphorylation. This regulatory mechanism has an important impact on cell differentiation.
- CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells
Summary: CREB3L1 has a role during the adaptation of the secretory pathway in response to high secretory demand in thyroid cells, a process with direct impact on thyroid physiology.
- RhoC regulates the actin remodeling required for phagosome formation during FcγR-mediated phagocytosis
Summary: RhoC accumulates at phagocytic cups and modulates an mDia1-dependent remodeling of cortical F-actin that is required for FcγR-mediated phagocytosis in macrophages.
- Quantitative analysis of multilayer organization of proteins and RNA in nuclear speckles at super resolution
Summary: Multi-color structured illumination microscopy imaging studies reveal a multilayer organization of nuclear speckles due to the interplay between favorable sequence-encoded intermolecular interactions of speckle-resident proteins and RNAs.
TOOLS AND RESOURCES
- In situ imaging of mitochondrial translation shows weak correlation with nucleoid DNA intensity and no suppression during mitosis
Highlighted Article: Imaging de novo mitochondrial protein synthesis with a novel fluorescence method shows the translational output throughout the cell and reveals a low correlation with mitochondrion genome copy number.
- ImaEdge – a platform for quantitative analysis of the spatiotemporal dynamics of cortical proteins during cell polarization
Summary: An open-source software package, ImaEdge, automates the segmentation of the cell cortex from time-lapse movies, and enables quantitative extraction of cortical protein dynamics in cells undergoing polarization.
- Real-time observation of light-controlled transcription in living cells
Summary: Blue light-induced chromatin recruitment (BLInCR) enables rapid and reversible targeting of effector proteins to nuclear loci with high temporal resolution for analyzing transcription activation.
This issue
JUMP TO
Other journals from The Company of Biologists
Cell science at a glance – The actin cortex at a glance
This new poster and article from Priyamvada Chugh and Ewa K. Paluch summarises the composition, architecture, mechanics and function of the cellular actin cortex, which determines the shape of animal cells, and, thus, provides the foundation for cell and tissue morphogenesis.
An interview with the preLights community manager, Máté Pálfy
preLights has a dedicated team of scientists who regularly select, highlight and comment on preprints that they feel are of interest to the biological community. We caught up with Máté Pálfy, the community manager of preLights, to talk about this service and his role in overseeing it.
We are also pleased to announce that Máté will be selecting and sharing the preLights he thinks are of the most interest to the cell science community, which JCS will feature as part of each issue's Table of Contents.
Review – Understanding eukaryotic chromosome segregation
In this new Review, Snezhana Oliferenko discusses eukaryotic chromosome segregation in the light of biological diversity, covering a number of experimental approaches used in comparative biology studies.
Research highlight – A direct role for AMPK in EMT
There have been conflicting results regarding the involvement of AMP-activated protein kinase (AMPK) in the process of epithelial-to-mesenchymal transition (EMT). In a new Research Article, Annapoorni Rangarajan and colleagues establish a direct role for AMPK through the upregulation of Twist1, and also point to a possible new strategic avenue to counteract cancer invasion and metastasis.
Meet the Team!
Will you be attending the Plant and Microbial Cytoskeleton GRC at Proctor Academy in Andover, NH from 12-17 August? Please look out for our Features & Reviews Editor Manuel Breuer and say hello!
JCS Meeting – Cell dynamics: organelle-cytoskeleton interface
The second in our series of cell dynamics meetings now turns to organelles. This May 2019 meeting in Lisbon, Portugal, aims to bring together scientists studying the interface between organelles and the cytoskeleton at different scales and perspectives using a range of model systems. Find out more and register your interest here. Registration opens in September.
preLights – Light at the end of the tunnel?
Gautam Dey highlights a preprint from David Stephens and colleagues that describes a new reporter for pulsed collagen secretion, providing support for a direct route from ER to Golgi. The preLight also features a response from the authors on the potential of their tool to define different aspects of procollagen trafficking, and opening the door for a machine-learning collaboration.
Articles of interest in our sister journals
The Drosophila insulin pathway controls Profilin expression and dynamic actin-rich protrusions during collective cell migration
Christian Ghiglione, Patrick Jouandin, Delphine Cérézo, Stéphane Noselli. Development 2018 145: dev161117
In vivo analysis of cardiomyocyte proliferation during trabeculation
Veronica Uribe, Radhan Ramadass, Deepika Dogra, S. Javad Rasouli, Felix Gunawan, Hiroyuki Nakajima, Ayano Chiba, Sven Reischauer, Naoki Mochizuki, Didier Y. R. Stainier. Development 2018 145: dev164194