A series of monoclonal antibodies binding to different epitopes shared by a 14 × 10(3)Mr membrane-bound polypeptide has been obtained. By indirect immuno-fluorescence, it was shown that the 14 × 10(3)Mr antigen is present in various cell types in Torpedo electric organ and muscle, especially fibroblasts, capillary endothelial cells, axonal cuff cells and, to a lesser extent, Schwann cells. At the electron-microscope level, after immunogold labelling, the antigen was found associated with the external surface of the plasma membrane of these cells, with the exception of the axonal cuff cells where part of the labelling was intracellular. The possible biological role of this 14 × 10(3)Mr protein is unknown but preliminary experiments suggest that this antigen has affinity for other Torpedo electric organ membrane proteins.
- © 1991 by Company of Biologists
Article navigation
Related articles
Cited by...
More in this TOC section
Similar articles
Other journals from The Company of Biologists
Cell scientist to watch – Kevin Corbett
"[...]the most compelling science emerges when you put together a set of people with different expertise and outlooks on a problem.”
Kevin heads a research group at UC San Diego and the Ludwig Institute for Cancer Research, investigating meiosis and its links to carcinogenesis. He shares his thoughts on fostering successful collaborations and why momentum is key to getting a new lab up and running.
Cell science at a glance – Cellular functions of WASP family proteins at a glance
In this poster and accompanying article, Olga Alekhina, Ezra Burstein and Daniel D. Billadeau provides insight into new functions of WASP family proteins from regulating the biogenesis of autophagosomes to recently identified roles in the nucleus.
Sticky wicket – Peerless II
Mole continues his philosophising on grant applications, this time delving into how to frame your project to capture the imaginations of the review panel.
Have you met our resident insectivore? Read more of his manic musings here.
Meeting report – Emerging concepts in cell organization
In January this year, the 10th Annaberg EMBO workshop was held in a medieval castle in Goldegg am See, Austria. David Teis and Wanda Kukulski report back on this lively exchange of the ideas and new concepts shaping our understanding of how cells are organized and how this organization impacts cellular functions.
Transfer to Biology Open
If your submission to Journal of Cell Science is unsuccessful, did you know you can transfer your paper and any reviews directly to our sister journal, Biology Open? The majority of papers transferred with reviews are accepted for publication. Find out how here.