Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Workshops and Meetings
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Alerts
  • Contact
    • Contact JCS
    • Subscriptions
    • Advertising
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in

Search

  • Advanced search
Journal of Cell Science
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Journal of Cell Science

  • Log in
Advanced search

RSS   Twitter  Facebook   YouTube  

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Workshops and Meetings
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Alerts
  • Contact
    • Contact JCS
    • Subscriptions
    • Advertising
    • Feedback
Research Article
Kinesin-1 sorting in axons controls differential retraction of arbor terminals
Takeshi Seno, Tatsuki Ikeno, Kousuke Mennya, Masayuki Kurishita, Narumi Sakae, Makoto Sato, Hiroki Takada, Yoshiyuki Konishi
J Cell Sci 2016 : jcs.183806 doi: 10.1242/jcs.183806 Published 5 August 2016
Takeshi Seno
Department of Human and Artificial Intelligence Systems, Faculty of Engineering, University of Fukui, Fukui 910-8507, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Takeshi Seno
Tatsuki Ikeno
Department of Human and Artificial Intelligence Systems, Faculty of Engineering, University of Fukui, Fukui 910-8507, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Tatsuki Ikeno
Kousuke Mennya
Department of Human and Artificial Intelligence Systems, Faculty of Engineering, University of Fukui, Fukui 910-8507, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kousuke Mennya
Masayuki Kurishita
Department of Human and Artificial Intelligence Systems, Faculty of Engineering, University of Fukui, Fukui 910-8507, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Masayuki Kurishita
Narumi Sakae
Department of Human and Artificial Intelligence Systems, Faculty of Engineering, University of Fukui, Fukui 910-8507, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Narumi Sakae
Makoto Sato
Life Science Innovation Center, University of Fukui, Fukui 910-8507, JapanResearch Center for Child Mental Development, University of Fukui, Fukui, 910-1193, JapanDepartment of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, Osaka, 565-0871, JapanUnited Graduate School of Child Development, Osaka University, Kanazawa University-Hamamatsu University School of Medicine, Chiba University and University of Fukui, Osaka University, Osaka 565-0871, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hiroki Takada
Department of Human and Artificial Intelligence Systems, Faculty of Engineering, University of Fukui, Fukui 910-8507, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yoshiyuki Konishi
Department of Human and Artificial Intelligence Systems, Faculty of Engineering, University of Fukui, Fukui 910-8507, JapanDepartment of Materials Science and Biotechnology, Faculty of Engineering, University of Fukui, Fukui 910-8507, JapanLife Science Innovation Center, University of Fukui, Fukui 910-8507, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Yoshiyuki Konishi
  • For correspondence: ykonishi@u-fukui.ac.jp
  • Article
  • Figures & tables
  • Supp info
  • Info & metrics
  • PDF + SI
  • PDF
Loading

Abstract

The ability of neurons to generate multiple arbor terminals from a single axon is crucial for establishing proper neuronal wiring. While growth and retraction of arbor terminals are differentially regulated within the axon, the mechanisms by which neurons locally control their structure remain largely unknown. In the present study, we found that the head domain of kinesin-1 (K5H) preferentially marks a subset of arbor terminals. Time-lapse imaging clarified that these arbor terminals are more stable than others, because of low retraction rate. Local inhibition of kinesin-1 in the arbor terminal by chromophore-assisted light inactivation (CALI) enhanced retraction rate. The microtubule turnover is locally regulated depending on the length from the branching point to the terminal end, but does not directly correlate with K5H. Conversely, F-actin signal values in arbor terminals correlate spatiotemporally with K5H, and inhibition of actin turnover prevented retraction. Results from the present study revealed a novel system mediated by kinesin-1 sorting in axons, which differentially controls stability of arbor terminals.

  • Received November 23, 2015.
  • Accepted August 2, 2016.
  • © 2016. Published by The Company of Biologists Ltd
Next Article
Back to top
Next Article

This Issue

 Download PDF

Email

Thank you for your interest in spreading the word on Journal of Cell Science.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Kinesin-1 sorting in axons controls differential retraction of arbor terminals
(Your Name) has sent you a message from Journal of Cell Science
(Your Name) thought you would like to see the Journal of Cell Science web site.
Share
Kinesin-1 sorting in axons controls differential retraction of arbor terminals
Takeshi Seno, Tatsuki Ikeno, Kousuke Mennya, Masayuki Kurishita, Narumi Sakae, Makoto Sato, Hiroki Takada, Yoshiyuki Konishi
J Cell Sci 2016 : jcs.183806 doi: 10.1242/jcs.183806 Published 5 August 2016
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Kinesin-1 sorting in axons controls differential retraction of arbor terminals
Takeshi Seno, Tatsuki Ikeno, Kousuke Mennya, Masayuki Kurishita, Narumi Sakae, Makoto Sato, Hiroki Takada, Yoshiyuki Konishi
J Cell Sci 2016 : jcs.183806 doi: 10.1242/jcs.183806 Published 5 August 2016

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
  • Figures & tables
  • Supp info
  • Info & metrics
  • PDF + SI
  • PDF

Related articles

Cited by...

More in this TOC section

  • The Arf-GDP-regulated recruitment of GBF1 to Golgi membranes requires domains HDS1 and HDS2 and a Golgi-localized protein receptor
  • Drp1 polymerization stabilizes curved tubular membranes similar to those of constricted mitochondria
  • The phospholipase A2 pathway controls a synaptic cholesterol ester cycle and synapse damage
Show more RESEARCH ARTICLE

Similar articles

Subject collections

  • Adhesion

Other journals from The Company of Biologists

Development

Journal of Experimental Biology

Disease Models & Mechanisms

Biology Open

Advertisement

Call for papers – Reconstituting cell biology

Deadline for submissions: 1 May 2018

We are now accepting submissions for our upcoming special issue on ‘Reconstituting cell biology’, guest edited by Manuel Théry. Click here for more information.


Opinion – The triple helix of collagens

In a new Opinion article, Billy G. Hudson and colleagues argue that the triple helix is an ancient protein structure of fundamental importance in building the extracellular matrix that enabled animal multicellularity and tissue evolution.


JCS Meeting – Cell dynamics: organelle-cytoskeleton interface

The second in our series of cell dynamics meetings now turns to organelles. This May 2019 meeting in Lisbon, Portugal, aims to bring together scientists studying the interface between organelles and the cytoskeleton at different scales and perspectives using a range of model systems. Find out more and register your interest here.


Review – Dynamics of cortical domains in early Drosophila development

Cortical domains are a central feature of epithelial and polarised cells. Anja Schmidt and Jörg Grosshans present a Review of the dynamics of cortical domains in early Drosophila embryos and discuss underlying mechanisms for formation of cortical domains.


Cell scientist to watch – 2018 Hooke medal winner Andrew McAinsh

“…identify an interesting, good scientific question and try to answer it.”

Andrew McAinsh, Head of Division of Biomedical Sciences at Warwick Medical School, is interested in understanding how the chromosomal multi-protein complex, the kinetochore, ensures error-free chromosome segregation. He is the recipient of the 2018 Hooke medal, established to recognize an emerging leader in cell biology.

We also feature interviews with first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Check out our recent First Person interviews with Thibault Courtheoux and Alghassimou Diallo, Francesco Consolato and Emily Herman.


Articles of interest in our sister journals

Head formation requires Dishevelled degradation that is mediated by March2 in concert with Dapper1
Hyeyoon Lee, Seong-Moon Cheong, Wonhee Han, Youngmu Koo, Saet-Byeol Jo, Gun-Sik Cho, Jae-Seong Yang, Sanguk Kim, Jin-Kwan Han. Development 2018 145: dev143107

PUF-8 facilitates homologous chromosome pairing by promoting proteasome activity during meiotic entry in C. elegans
Ganga Anil Kumar, Kuppuswamy Subramaniam. Development 2018 145: dev163949

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Interviews
  • Alerts

About us

  • About Journal of Cell Science
  • Editors and Board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Workshops and Meetings
  • The Company of Biologists

For Authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Figure preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • JCS Prize
  • Biology Open transfer

Journal Info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Alerts

Contact

  • Contact Journal of Cell Science
  • Subscriptions
  • Advertising
  • Feedback

Twitter   YouTube   LinkedIn

© 2018   The Company of Biologists Ltd   Registered Charity 277992