QuickTime Video Fig.2 Movie of fluorescence images of an Ax2 cell with an extending protrusion. Cells had been stained with RH-795 10 minutes before quinine application. This dye binds to contractile vacuole membranes with high affinity. Note the highly fluorescent patch in the leading edge of the protrusion.
QuickTime Video Fig.4 Movie showing concanavalin A-tagged beads attached to the cell surface during elongation of protrusion. Fluorescence is shown in red and Nomarski images in green.
<QuickTime Video Fig.6C Movie of fluorescence images of an mhc-null cell showing expulsion of a contractile vacuole. The cell had been stained with RH-795 10 minutes before quinine application. A patch of fluorescence persists on the cell membrane at the site of expulsion of one of the contractile vacuoles.