JCS057562 Supplementary Material
Files in this Data Supplement:
- Supplemental Figure S1
Fig. S1. Arp2/3 RNAi embryos express E and MS fate markers normally. (A,B) end-1::GFP, a marker for endodermal fate, was expressed normally in Arp2/3 RNAi embryos as the Ea/p cells divided on the surface (arrowhead, n=28/28). (C,D) ceh-51::GFP, a marker for MS cell fate appeared normal as Ea/p cells divided on the surface (n=19/19). Three of the four Ea/p cell descendants are marked by asterisks, as the fourth cell was not in the same imaging plane.
- Supplemental Figure S2
Fig. S2. The formation of F-actin-rich extensions is cell-fate dependent. Ventral views of embryo with MS descendants color-coded in pink, and E descendants color-coded in blue. Time is indicated as minutes after MS and E are born. (A,C,E,G,I) A wild-type embryo. (A) MSa/p cells have not yet divided, and Ea/p cells are still on the surface. (C,E) MSa/p cells divided and Ea/p cells internalization begins. Only three out of the four MS descendants are visible in the same plane. (G) By 46 minutes, Ea/p cells are covered by the MS descendants and other neighboring cells. (I) MS descendants have undergone another round of cell division on the surface of the embryo. (B,D,F,H,J) A pop-1 mutant embryo to show the MS to E cell fate transformation (n=5/5). (D) At 26 minutes, MSa/p cells have not yet divided. (F) By 36 minutes, MSa/p cells begin to divide, but have already started internalizing with the Ea/p cells. (H) At 46 minutes, Ea/p cells and several of the MS descendants have internalized. (I) At 56 minutes, MS and E descendants have internalized, but the neighboring cells have not fully sealed the gap. (K) A mid-plane DIC image of a pop-1 mutant embryo. MSx is labeled. (L) A surface view of the same pop-1-depleted embryo expressing GFP::MOE to show the neighboring cell/MSx cell boundary (white arrowhead) and MSx/Ea cell boundary (black arrowhead).
- Movie 1
Movie 1. Arp2/3 is required for stable membrane-cytoskeletal linkages at free cell surfaces. A cropped movie of the embryo expressing GFP::MOE and PH::mCherry shown in Fig. 3I,J. Frame rate is one frame every 3 seconds.
- Movie 2
Movie 2. Three of the six cells surrounding the Ea/p cells produce dynamic F-actin-rich structures. A movie of the wild-type GFP::MOE embryo shown in Fig. 6. The movie began at 6 minutes after MSa/p division. Frame rate is one frame every 3 seconds.
- Movie 3
Movie 3. The formation of the MSxx-specific F-actin-rich structures is Arp2/3 dependent. A movie of the Arp2/3-depleted embryo expressing GFP::MOE shown in Fig.7. The movie began at 6 minutes after MSa/p division. Frame rate is one frame every 3 seconds.