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About the Cover
Cover: 
-tubulin is abundant in developing spermatids in the male gametophyte of Marsilea vestita. Spermiogenesis is an 11-hour process in this organism and is initiated by placing dry spores into water. This gametophyte was fixed six hours after the microspore had been placed into water. The methacrylate-embedded material was sectioned, and the tissue was immunolabeled with anti--tubulin antibody and then with an immunogold-conjugated secondary antibody. The anti--tubulin antibody is almost exclusively localized to the cytoplasm of the developing spermatids in this normal gametophyte. In this section, >12 of the 32 spermatids are present and clustered into two groups within the spore wall. The unlabeled zones within the spermatids contain the gamete nuclei. The micrograph is a reflected light confocal image stack superimposed upon a transmitted DIC image. See article by Tsai and Wolniak (pp. 4265-4272).
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