Table 2.

Rescue of melan-ln melanosome transport defects by re-introduction of epitope-tagged Mlph following siRNA knockdown of Mlph-interacting proteins

siRNA transfected Number of cells analysed P-value vs positive control P-value vs negative control
Rab27a#1 42 0.00 0.95
Rab27a#2 36 0.00 0.41
MyoVa#1 38 0.00 0.53
MyoVa#2 34 0.00 0.17
EB1#2 78 0.56 0.00
EB1#3 61 0.46 0.00
  • Control cells were transfected first with non-targeted siRNA oligonucleotides and then again with plasmid allowing expression of Myc-Mlph (positive) or Myc-Slp1 (negative)