Table 3.

Aconitase-MTS elongation mutants

Plasmid Mutant protein MPII Score Hmax μH§ MTS sequence (3′ to 5′) Distribution Mito vs Cyt
WT-ACO1 WT-Aco1 0.9950 5.01 6.23 MLSARSAIKRPIVRGL*ATV 94:6
N MTS9aa Fum-Aco1 0.9989 4.26 5.78 MLSARSAIKRPIVRGYKLNIRRMNTV 50:50
0 MTS9aa Aco-Aco1 0.9996 5.48 8.54 MLSARSAIKRPIVRGKRPIVRGLATV 40:60
  • MitoProtII score (Claros et al., 1997)

  • Maximal hydrophobicity of the hydrophobic face of the helical structure (von Heijne, 1986)

  • § Hydrophobic moment (von Heijne, 1986)

  • Percentage of mitochondrial and cytosolic fractions. Modifications of the amino acid sequences are in bold letters

  • * , MPP cleavage site