Table 4.

Fumarase-MTS point mutants

Plasmids Mutant protein MPII Score Hmax μH§ MTS sequence (3′ to 5′) Distribution Mito vs Cyt
WT-FUM1 WT-Fum1 0.9265 4.56 8.38 MLRFTNCSCKTFVKSSYKLNIRRM*N 30:70
R MTS3Fum1 0.9291 4.56 6.08 MLSFTNCSCKTFVKSSYKLNIRRMN 58:42
S MTS5Fum1 0.9546 2.11 5.94 MLRFRNCSCKTFVKSSYKLNIRRMN 58:42
T MTS9Fum1 0.8856 3.04 7.33 MLRFTNCSKKTFVKSSYKLNIRRMN 50:50
  • MitoProtII score (Claros et al., 1997)

  • Maximal hydrophobicity of the hydrophobic face of the helical structure (von Heijne, 1986)

  • § Hydrophobic moment (von Heijne, 1986)

  • Percentage of mitochondrial and cytosolic fractions. Modifications of the amino acid sequences are in bold letters

  • * , MPP cleavage site