- Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA
Summary: Vimentin intermediate filaments control the activity of RhoA, and consequent stress fiber assembly and contractility by downregulating its guanine nucleotide exchange factor GEF-H1.
- The spectraplakin Short stop is an essential microtubule regulator involved in epithelial closure in Drosophila
Summary: The Drosophila spectraplakin Shot coordinates actin–microtubule interactions at the leading edge to ensure the formation of filopodia that promote the dorsal closure of the embryonic epithelium.
- RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells
Summary: The role of the small GTPase RhoA in the assembly of the apical surface in nascent multiciliated cells is explored, revealing that RhoA and formin 1 control forces acting on the apical surface to regulate apical emergence.
- Simultaneous quantification of actin monomer and filament dynamics with modeling-assisted analysis of photoactivation
Summary: We have devised a new modeling-based analysis for photoactivatable actin experiments that accurately measures multiple parameters of actin dynamics, providing more detailed results than those obtained using traditional half-time analysis.
- Perturbations in actin dynamics reconfigure protein complexes that modulate GCN2 activity and promote an eIF2 response
Highlighted Article: We have uncovered a cross-talk between the actin cytoskeleton and protein synthesis that is mediated by GCN2. Actin depolymerization affects specific protein–protein interactions that promote GCN2 function.
- A new isoform of Drosophila non-muscle Tropomyosin 1 interacts with Kinesin-1 and functions in oskar mRNA localization
Highlighted Article: A previously uncharacterized isoform of Tropomyosin, referred to as Tm1C, directly interacts with Kinesin heavy chain and functions along with this microtubule motor to localize oskar mRNA in Drosophila oocytes.
- Tensin 3 is a new partner of Dock5 that controls osteoclast podosome organization and activity
Summary: Tensin 3 regulates podosome patterning in osteoclasts and bone resorption. Here, we show that tensin 3 is a partner of the Rac exchange factor Dock5 that stimulates its activity.
- Persistent nuclear actin filaments inhibit transcription by RNA polymerase II
Highlighted Article: Depletion of actin in the nucleus by polymerization into stable nuclear actin filaments inhibits the actin–polymerase-II interaction, resulting in impaired RNA polymerase II activity, localization, and gene recruitment.
- Cellular functions of the ADF/cofilin family at a glance
Summary: This Cell Science at a Glance article and the accompanying poster serve as an introduction to the diverse roles of ADF and cofilin proteins in cells.