- Real-time insights into regulated exocytosis
Summary: This Commentary focuses on recent developments in three-dimensional time-lapse imaging of regulated secretion occurring in living secreting organs.
- Tension regulates myosin dynamics during Drosophila embryonic wound repair
Highlighted Article: We quantify non-muscle myosin II dynamics during the coordinated cell movements that drive embryonic wound repair, and demonstrate that mechanical forces and phosphorylation regulate myosin turnover.
- The spectraplakin Short stop is an essential microtubule regulator involved in epithelial closure in Drosophila
Summary: The Drosophila spectraplakin Shot coordinates actin–microtubule interactions at the leading edge to ensure the formation of filopodia that promote the dorsal closure of the embryonic epithelium.
- Drosophila WASH is required for integrin-mediated cell adhesion, cell motility and lysosomal neutralization
Summary: Drosophila wash was originally described as an essential gene. We show that wash mutant flies are viable and fertile, and that WASH has conserved functions in integrin receptor recycling and lysosome neutralization.
- Drosophila Sulf1 is required for the termination of intestinal stem cell division during regeneration
Highlighted Article: The heparan sulfate 6-O endosulfatase Sulf1 is required for proper inactivation of Drosophila intestinal stem cell division during regeneration by modulating mitogenic signaling pathways.
- Drosophila vinculin is more harmful when hyperactive than absent, and can circumvent integrin to form adhesion complexes
Summary: Development is more sensitive to gain of vinculin activity than its loss, and vinculin can promote cytoplasmic adhesion complexes independently of the usual integrin cue.
- Mutant IP3 receptors attenuate store-operated Ca2+ entry by destabilizing STIM–Orai interactions in Drosophila neurons
Summary: In Drosophila neurons, mutant IP3 receptors disrupt store-operated Ca2+ entry by destabilizing interaction of STIM with the Ca2+ channel, Orai. The interactions could coordinate store emptying with Ca2+ entry.
- In vivo regulation of integrin turnover by outside-in activation
Highlighted Article: Outside-in integrin activation regulates integrin turnover. Furthermore, integrin activation drives the developmental stabilization of cell–ECM adhesion by decreasing integrin turnover during embryogenesis.
- Does Unc–GFP uncover ciliary structures in the rhabdomeric eye of Drosophila?
Summary: The basal body marker Unc–GFP is found in the Drosophila eye imaginal discs that lack ciliary structures. This suggests new roles for the Unc protein.