- GSK3-mediated CLASP2 phosphorylation modulates kinetochore dynamics
Summary: Kinetochore–microtubule interactions are essential for faithful chromosome segregation. Here, we describe consequences of GSK3 phosphorylation-site mutations that control CLASP2 interactions with microtubules during mitosis.
- Alterations in the balance of tubulin glycylation and glutamylation in photoreceptors leads to retinal degeneration
Summary: The connecting cilia of photoreceptors are post-translationally glycylated and glutamylated. Unbalancing these two modifications invariably leads to retinal degeneration.
- The spectraplakin Short stop is an essential microtubule regulator involved in epithelial closure in Drosophila
Summary: The Drosophila spectraplakin Shot coordinates actin–microtubule interactions at the leading edge to ensure the formation of filopodia that promote the dorsal closure of the embryonic epithelium.
- MZT1 regulates microtubule nucleation by linking γTuRC assembly to adapter-mediated targeting and activation
Highlighted Article: In human cells, integrity of the microtubule nucleator γTuRC is crucial for γ-tubulin-dependent functions and is monitored by MZT1 to enable interaction with other factors necessary for spatial regulation of nucleation.
- Microtubules in 3D cell motility
Summary: We discuss microtubule functions that control cell shape and motility in 3D matrices, and the differences of microtubule involvement in cell morphogenesis and motility between 2D and 3D environments.
- Control of apico–basal epithelial polarity by the microtubule minus-end-binding protein CAMSAP3 and spectraplakin ACF7
- Triadin and CLIMP-63 form a link between triads and microtubules in muscle cells
Summary: CLIMP-63 is associated with the Ca2+ release complex of skeletal muscles through its interaction with triadin, and is involved in the shaping of triads and in the guidance of microtubules.
- Kinesin-1 sorting in axons controls the differential retraction of arbor terminals
Summary: Axonal transport of kinesin-1 and microtubule dynamics are differentially regulated between terminals in a single axon, and contribute to shaping the axonal arbor by differentially regulating retraction of terminals.
- A role for the yeast CLIP170 ortholog, the plus-end-tracking protein Bik1, and the Rho1 GTPase in Snc1 trafficking
Summary: In the yeast Saccharomyces cerevisiae, cargo trafficking involves microtubule plus-ends and a Bik1-dependent mechanism that requires the presence of the C-terminal aromatic residue of β-tubulin.
- CYLD – a deubiquitylase that acts to fine-tune microtubule properties and functions
Summary: This Commentary focuses on recent literature that shows how CYLD impacts on microtubule properties and functions, and discusses the challenges we face when interpreting results obtained from different experimental systems.