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Journal Articles
Association of p34cdc2/cyclin B complex with microtubules in starfish oocytes
K. Ookata, S. Hisanaga, E. Okumura, T. Kishimoto
Journal of Cell Science 1993 105: 873-881;
K. Ookata
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S. Hisanaga
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E. Okumura
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T. Kishimoto
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Summary

The microtubular cytoskeleton exhibits a dramatic reorganization, progressing from interphase radial arrays to a mitotic spindle at the G2/M transition. Although this reorganization has been suspected to be caused by maturation promoting factor (MPF: p34cdc2/cyclin B complex), little is known about how p34cdc2 kinase controls microtubule networks. We provide evidence of the direct association of the p34cdc2/cyclin B complex with microtubules in starfish oocytes. Anti-cyclin B staining of detergent-treated oocytes, isolated asters and meiotic spindles revealed fluorescence associated with microtubule fibers, chromosomes and centrosomes. Microtubules prepared from starfish oocytes were associated with cyclin B and p34cdc2 proteins. Microtubule-bound p34cdc2 and cyclin B were released from microtubules by a high-salt solution and possessed a complex form as shown by the adsorption to suc1-beads and by immunoprecipitation with the anti-cyclin B antibody. The p34cdc2/cyclin B complex associated to microtubules had high histone H1 kinase activity at meiotic metaphase. However, it was not necessary for the p34cdc2/cyclin B complex to be active for microtubule binding, as an inactive form in immature oocytes was also observed to bind to microtubules. The coprecipitation of suc1-column purified p34cdc2/cyclin B with purified porcine brain microtubules in the presence of starfish oocyte microtubule-associated proteins (MAPs) indicates that the association of p34cdc2/cyclin B with microtubules in vitro is mediated by MAPs.

  • © 1993 by Company of Biologists

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Association of p34cdc2/cyclin B complex with microtubules in starfish oocytes
K. Ookata, S. Hisanaga, E. Okumura, T. Kishimoto
Journal of Cell Science 1993 105: 873-881;
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Association of p34cdc2/cyclin B complex with microtubules in starfish oocytes
K. Ookata, S. Hisanaga, E. Okumura, T. Kishimoto
Journal of Cell Science 1993 105: 873-881;

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