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A gradient method for the quantitative analysis of cell movement and tissue flow and its application to the analysis of multicellular Dictyostelium development
F. Siegert, C.J. Weijer, A. Nomura, H. Miike
Journal of Cell Science 1994 107: 97-104;
F. Siegert
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C.J. Weijer
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A. Nomura
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H. Miike
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Summary

We describe the application of a novel image processing method, which allows quantitative analysis of cell and tissue movement in a series of digitized video images. The result is a vector velocity field showing average direction and velocity of movement for every pixel in the frame. We apply this method to the analysis of cell movement during different stages of the Dictyostelium developmental cycle. We analysed time-lapse video recordings of cell movement in single cells, mounds and slugs. The program can correctly assess the speed and direction of movement of either unlabelled or labelled cells in a time series of video images depending on the illumination conditions. Our analysis of cell movement during multicellular development shows that the entire morphogenesis of Dictyostelium is characterized by rotational cell movement. The analysis of cell and tissue movement by the velocity field method should be applicable to the analysis of morphogenetic processes in other systems such as gastrulation and neurulation in vertebrate embryos.

  • © 1994 by Company of Biologists

REFERENCES

    1. Bonner J.,
    2. Hall D. and
    3. Suthers H.
    (1989). Ammonia and thermotaxis- further evidence for a central role of ammonia in the directed cell mass movements of Dictyostelium discoideum. Proc. Nat. Acad. Sci. USA 86, 2733–2736
    OpenUrlAbstract/FREE Full Text
    1. Clark R. L. and
    2. Steck T. L.
    (1979). Morphogenesis in Dictyostelium: An orbital hypothesis. Science 204, 1163–1168
    OpenUrlAbstract/FREE Full Text
    1. Condeelis J.,
    2. Bresnick A.,
    3. Demma M.,
    4. Dharmawardhane S.,
    5. Eddy R.,
    6. Hall A. L.,
    7. Sauterer R. and
    8. Warren V.
    (1990). Mechanisms of amoeboid chemotaxis: an evaluation of the cortical expansion model. Dev. Genet 11, 333–340
    OpenUrlCrossRefPubMedWeb of Science
    1. Durston A. J. and
    2. Vork F.
    (1979). A cinematographical study of the development of vitally stained Dictyostelium discoideum. J. Cell Sci 36, 261–279
    OpenUrlAbstract/FREE Full Text
    1. Elliot S.,
    2. Joss G. H.,
    3. Spudich A. and
    4. Williams K. L.
    (1993). Patterns in Dictyostelium discoideum: the role of myosin II in the transition from the unicellular to the multicellular phase. J. Cell Sci 104, 457–466
    OpenUrlAbstract/FREE Full Text
    1. Fisher P.,
    2. Merkl R. and
    3. Gerisch G.
    (1989). Quantitative analysis of cell motility and chemotaxis in Dictyostelium discoideum by using an image processing system and a novel chemotaxis chamber providing stationary chemical gradients. J. Cell Biol 108, 973–984
    OpenUrlAbstract/FREE Full Text
    1. Horn B. K. P. and
    2. Schunk B. G.
    (1981). Determining optical flow. Artificial Intelligence 17, 185–203
    OpenUrlCrossRefWeb of Science
    1. Rubin J. and
    2. Robertson A.
    (1975). The tip of the Dictyostelium discoideum pseudoplasmodium as an organizer. J. Embryol. Exp. Morph 33, 1–.
    OpenUrlPubMedWeb of Science
    1. Segall J.
    (1988). Quantification of motility and area changes of Dictyostelium discoideum amoebas in response to chemoattractants. J. Muscle Res. Cell Motil 9, 481–490
    OpenUrlCrossRefPubMedWeb of Science
    1. Segall J. E.
    (1992). Behavioral responses of streamer-F Mutants of Dictyostelium-discoideum -effects of cyclic GMP on cell motility. J. Cell Sci 101, 589–597
    OpenUrlAbstract/FREE Full Text
    1. Siegert F. and
    2. Weijer C. J.
    (1992). Three-dimensional scroll waves organize Dictyostelium slugs. Proc. Nat. Acad. Sci. USA 89, 6433–6437
    OpenUrlAbstract/FREE Full Text
    1. Steinbock O.,
    2. Siegert F.,
    3. Muller S. C. and
    4. Weijer C. J.
    (1993). Three-dimensional waves of excitation during Dictyostelium morphogenesis. Proc. Nat. Acad. Sci. USA 90, 7332–7335
    OpenUrlAbstract/FREE Full Text
    1. Varnum B.,
    2. Edwards K. B. and
    3. Soll D. R.
    (1985). Dictyostelium amoebae alter motility differently in response to increasing versus decreasing temporal gradient of cAMP. J. Cell Biol 101, 1–5
    OpenUrlAbstract/FREE Full Text
    1. Wessels D.,
    2. Soll D.,
    3. Knecht D.,
    4. Loomis W.,
    5. Delozanne A. and
    6. Spudich J.
    (1988). Cell motility and chemotaxis in Dictyostelium amoebae lacking myosin heavy-chain. Dev Biol 128, 164–177
    OpenUrlCrossRefPubMedWeb of Science
    1. Wessels D.,
    2. Murray J. and
    3. Soll D. R.
    (1992). Behavior of Dictyostelium amoebae is regulated promarely by the temporal dynamic of the natural cAMP wave. Cell Motil. Cytoskel 23, 145–156
    OpenUrlCrossRefPubMed
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Journal Articles
A gradient method for the quantitative analysis of cell movement and tissue flow and its application to the analysis of multicellular Dictyostelium development
F. Siegert, C.J. Weijer, A. Nomura, H. Miike
Journal of Cell Science 1994 107: 97-104;
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Journal Articles
A gradient method for the quantitative analysis of cell movement and tissue flow and its application to the analysis of multicellular Dictyostelium development
F. Siegert, C.J. Weijer, A. Nomura, H. Miike
Journal of Cell Science 1994 107: 97-104;

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