Advertisement
Journal Articles
Identification of MYO4, a second class V myosin gene in yeast
B.K. Haarer, A. Petzold, S.H. Lillie, S.S. Brown
Journal of Cell Science 1994 107: 1055-1064;

Summary

We have isolated a fourth myosin gene (MYO4) in yeast (Saccharomyces cerevisiae). MYO4 encodes a approximately 170 kDa (1471 amino acid) class V myosin, using the classification devised by Cheney et al. (1993a; Cell Motil. Cytoskel. 24, 215–223); the motor domain is followed by a neck region containing six putative calmodulin-binding sites and a tail with a short potential ‘coiled-coil’ domain. A comparison with other myosins in GenBank reveals that Myo4 protein is most closely related to the yeast Myo2 protein, another class V myosin. Deletion of MYO4 produces no detectable phenotype, either alone or in conjunction with mutations in myo2 or other myosin genes, the actin gene, or secretory genes. However, overexpression of MYO4 or MYO2 results in several morphological abnormalities, including the formation of short strings of unseparated cells in diploid strains, or clusters of cells in haploid strains. Alterations of MYO4 or MYO2 indicate that neither the motor domains nor tails of these myosins are required to confer the overexpression phenotype, whereas the neck region may be required. Although this phenotype is similar to that seen upon MYO1 deletion, we provide evidence that the overexpression of Myo4p or Myo2p is not simply interfering with Myo1p function.

REFERENCES

    1. Cheney R. E. and
    2. Mooseker M. S.
    (1992). Unconventional myosins. Curr. Opin. Cell Biol 4, 27–35
    OpenUrlCrossRefPubMed
    1. Cheney R. E.,
    2. Riley M. A. and
    3. Mooseker M. S.
    (1993). Phylogenetic analysis of the myosin superfamily. Cell Motil. Cytoskel 24, 215–223
    OpenUrlCrossRefPubMedWeb of Science
    1. Cheney R. E.,
    2. O'Shea M. K.,
    3. Heuser J. E.,
    4. Coelho M. V.,
    5. Wolenski J. S.,
    6. Espreafico E. M.,
    7. Forscher P.,
    8. Larson R. E. and
    9. Mooseker M. S.
    (1993). Brain myosin-V is a two-headed unconventional myosin with motor activity. Cell 75, 13–23
    OpenUrlCrossRefPubMedWeb of Science
    1. Cohen C. and
    2. Parry D. A. D.
    (1990). -helical coiled coils and bundles: how to design an -helical protein. Proteins 7, 1–15
    OpenUrlCrossRefPubMedWeb of Science
    1. Davis T. N.
    (1992). A temperature-sensitive calmodulin mutant loses viability during mitosis. J. Cell Biol 118, 607–617
    OpenUrlAbstract/FREE Full Text
    1. Devereux J.,
    2. Haeberli P. and
    3. Smithies O.
    (1984). A comprehensive set of sequence analysis programs for the VAX. Nucl. Acids Res 12, 387–395
    OpenUrlAbstract/FREE Full Text
    1. Diehl B. E. and
    2. Pringle J. R.
    (1991). Molecular analysis of Saccharomyces cerevisiae chromosome I: identification of additional transcribed regions and demonstration that some encode essential functions. Genetics 127, 287–298
    OpenUrlAbstract/FREE Full Text
    1. Espindola F. S.,
    2. Espreafico E. M.,
    3. Coelho M. V.,
    4. Martins A. R.,
    5. Costa F. R.,
    6. Mooseker M. S. and
    7. Larson R. E.
    (1992). Biochemical and immunological characterization of p190-calmodulin complex from vertebrate brain: a novel calmodulin-binding myosin. J. Cell Biol 118, 359–368
    OpenUrlAbstract/FREE Full Text
    1. Espreafico E. M.,
    2. Cheney R. E.,
    3. Matteoli M.,
    4. Nascimento A. A. C.,
    5. De Camilli P. V.,
    6. Larson R. E. and
    7. Mooseker M. S.
    (1992). Primary structure and cellular localization of chicken brain myosin-V (p190), an unconventional myosin with calmodulin light chains. J. Cell Biol 119, 1541–1557
    OpenUrlAbstract/FREE Full Text
    1. Feinberg A. P. and
    2. Vogelstein B.
    (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem 132, 6–13
    OpenUrlCrossRefPubMedWeb of Science
    1. Gerst J. E.,
    2. Rodgers L.,
    3. Riggs M. and
    4. Wigler M.
    (1992). SNC1, a yeast homolog of the synaptic vesicle-associated membrane protein/ synaptobrevin gene family: genetic interactions with the RAS and CAP genes. Proc. Nat. Acad. Sci. USA 89, 4338–4342
    OpenUrlAbstract/FREE Full Text
    1. Goodson H. V.,
    2. Titus M. A. and
    3. Spudich J. A.
    (1990). Disruption of a yeast unconventional myosin. J. Cell Biol 111, 168–.
    OpenUrl
    1. Govindan B.,
    2. Bowser R. and
    3. Novick P.
    (1991). Role of the unconventional myosin gene MYO2 in the yeast secretory pathway. J. Cell Biol 115, 185–.
    OpenUrl
    1. Haarer B. K. and
    2. Pringle J. R.
    (1987). Immunofluorescence localization of the Saccharomyces cerevisiaeCDC12 gene product to the vicinity of the 10-nm filaments in the mother-bud neck. Mol. Cell. Biol 7, 3678–3687
    OpenUrlAbstract/FREE Full Text
    1. Haarer B. K.,
    2. Lillie S. H.,
    3. Adams A. E.,
    4. Magdolen V.,
    5. Bandlow W. and
    6. Brown S. S.
    (1990). Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells. J. Cell Biol 110, 105–114
    OpenUrlAbstract/FREE Full Text
    1. Hicks J. B.,
    2. Strathern J. N. and
    3. Herskowitz I.
    (1977). Interconversion of yeast mating types III. Action of homothalism (HO) gene in cells homozygous for the mating type locus. Genetics 85, 395–405
    OpenUrlAbstract/FREE Full Text
    1. Hill J. E.,
    2. Myers A. M.,
    3. Koerner T. J. and
    4. Tzagoloff A.
    (1986). Yeast/ E. coli shuttle vectors with multiple unique restriction sites. Yeast 2, 163–167
    OpenUrlCrossRefPubMedWeb of Science
    1. Johnston G. C.,
    2. Prendergast J. A. and
    3. Singer R. A.
    (1991). The Saccharomyces cerevisiaeMYO2 gene encodes an essential myosin for vectorial transport of vesicles. J. Cell Biol 113, 539–551
    OpenUrlAbstract/FREE Full Text
    1. Kim H. B.,
    2. Haarer B. K. and
    3. Pringle J. R.
    (1991). Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC3 gene product and the timing of events at the budding site. J. Cell Biol 112, 535–544
    OpenUrlAbstract/FREE Full Text
    1. Larson R. E.,
    2. Pitta D. E. and
    3. Ferro J. A.
    (1988). A novel 190 kDa calmodulin-binding protein associated with brain actomyosin. Brazilian J. Med. Biol. Res 21, 213–217
    OpenUrlPubMedWeb of Science
    1. Lillie S. H. and
    2. Pringle J. R.
    (1980). Reserve carbohydrate metabolism in Saccharomyces cerevisiae: responses to nutrient limitation. J. Bacteriol 143, 1384–1394
    OpenUrlAbstract/FREE Full Text
    1. Lillie S. H. and
    2. Brown S. S.
    (1992). Suppression of a myosin defect by a kinesin-related gene. Nature 356, 358–361
    OpenUrlCrossRefPubMedWeb of Science
    1. Lupas A.,
    2. Van Dyke M. and
    3. Stock J.
    (1991). Predicting coiled coils from protein sequences. Science 252, 1162–1164
    OpenUrlFREE Full Text
    1. Mercer J. A.,
    2. Seperack P. K.,
    3. Strobel M. C.,
    4. Copeland N. G. and
    5. Jenkins N. A.
    (1991). Novel myosin heavy chain encoded by murine dilute coat colour locus (see correction Nature 352, 547). Nature 349, 709–712
    OpenUrlCrossRefPubMedWeb of Science
    1. Pringle J. R.,
    2. Adams A. E. M.,
    3. Drubin D. G. and
    4. Haarer B. K.
    (1991). Immunofluorescence methods for yeast. Meth. Enzymol 194, 565–602
    OpenUrlCrossRefPubMedWeb of Science
    1. Rayment I.,
    2. Rypniewski W. R.,
    3. Schmidt-Base K.,
    4. Smith R.,
    5. Tomchick D. R.,
    6. Benning M. M.,
    7. Winkelmann D. A.,
    8. Wesenberg G. and
    9. Holden H. M.
    (1993). Three-dimensional structure of myosin subfragment-1: a molecular motor. Science 261, 50–58
    OpenUrlAbstract/FREE Full Text
    1. Roberts C. J.,
    2. Raymond C. K.,
    3. Yamashiro C. T. and
    4. Stevens T. H.
    (1991). Methods for studying the yeast vacuole. Meth. Enzymol 194, 644–661
    OpenUrlCrossRefPubMedWeb of Science
    1. Rodriguez J. R. and
    2. Paterson B. M.
    (1990). Yeast myosin heavy chain mutant: maintenance of the cell type specific budding pattern and the normal deposition of chitin and cell wall components requires an intact myosin heavy chain gene. Cell Motil. Cytoskel 17, 301–308
    OpenUrlCrossRefPubMedWeb of Science
    1. Rose M. D.,
    2. Novick P.,
    3. Thomas J. H.,
    4. Botstein D. and
    5. Fink G. R.
    (1987). A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60, 237–243
    OpenUrlCrossRefPubMedWeb of Science
    1. Rothstein R. J.
    (1983). One-step gene disruption in yeast. Meth. Enzymol 101, 202–211
    OpenUrlCrossRefPubMedWeb of Science
    1. Ruther U. and
    2. Muller-Hill B.
    (1983). Easy identification of cDNA clones. EMBO J 2, 1791–1794
    OpenUrlPubMedWeb of Science
    1. Sanger F.,
    2. Nicklin S. and
    3. Coulson A. R.
    (1977). DNA sequencing with chain-terminating inhibitors. Proc. Nat. Acad. Sci. USA 74, 5463–5467
    OpenUrlAbstract/FREE Full Text
    1. Sloat B. F.,
    2. Adams A. and
    3. Pringle J. R.
    (1981). Roles of the CDC24 gene product in cellular morphogenesis during the Saccharomyces cerevisiae cell cycle. J. Cell Biol 89, 395–405
    OpenUrlAbstract/FREE Full Text
    1. Watts F. Z.,
    2. Shiels G. and
    3. Orr E.
    (1987). The yeast MYO1 gene encoding a myosin-like protein required for cell division. EMBO J 6, 3499–3505
    OpenUrlPubMedWeb of Science
Back to top

 Download PDF

Email
Journal Articles
Identification of MYO4, a second class V myosin gene in yeast
B.K. Haarer, A. Petzold, S.H. Lillie, S.S. Brown
Journal of Cell Science 1994 107: 1055-1064;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Alerts

Article navigation

Other journals from The Company of Biologists

Advertisement

2020 at The Company of Biologists

Despite the challenges of 2020, we were able to bring a number of long-term projects and new ventures to fruition. While we look forward to a new year, join us as we reflect on the triumphs of the last 12 months.

Mole – The Corona Files

"This is not going to go away, 'like a miracle.' We have to do magic. And I know we can."

Mole continues to offer his wise words to researchers on how to manage during the COVID-19 pandemic.

Cell scientist to watch – Christine Faulkner

In an interview, Christine Faulkner talks about where her interest in plant science began, how she found the transition between Australia and the UK, and shares her thoughts on virtual conferences.

Read & Publish participation extends worldwide

“The clear advantages are rapid and efficient exposure and easy access to my article around the world. I believe it is great to have this publishing option in fast-growing fields in biomedical research.”

Dr Jaceques Behmoaras (Imperial College London) shares his experience of publishing Open Access as part of our growing Read & Publish initiative. We now have over 60 institutions in 12 countries taking part – find out more and view our full list of participating institutions.

JCS and COVID-19

For more information on measures Journal of Cell Science is taking to support the community during the COVID-19 pandemic, please see here.

If you have any questions or concerns, please do not hestiate to contact the Editorial Office.