Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Cell Scientists to Watch
    • First Person
    • Sign up for alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Fast-track manuscripts
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Manuscript transfer network
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contact JCS
    • Subscriptions
    • Advertising
    • Feedback
    • Institutional usage stats (logged-in users only)
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in
  • Log out

Search

  • Advanced search
Journal of Cell Science
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Journal of Cell Science

  • Log in
Advanced search

RSS   Twitter  Facebook   YouTube  

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Cell Scientists to Watch
    • First Person
    • Sign up for alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Fast-track manuscripts
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Manuscript transfer network
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contact JCS
    • Subscriptions
    • Advertising
    • Feedback
    • Institutional usage stats (logged-in users only)
Journal Articles
Evidence that tenascin and thrombospondin-1 modulate sprouting of endothelial cells
A.E. Canfield, A.M. Schor
Journal of Cell Science 1995 108: 797-809;
A.E. Canfield
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A.M. Schor
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & metrics
  • PDF
Loading

Summary

Cultured endothelial cells undergo a reversible transition from a resting (cobblestone) phenotype to an angiogenic (sprouting) phenotype. This transition mimics the early events of angiogenesis. We have previously reported that the addition of exogenous xylosides inhibits endothelial cel sprouting and modifies the extracellular matrix (ECM) synthesised by the cells. We have now investigated whether endothelial sprouting is mediated by the nature of the extracellular matrix in contact with the cells. Accordingly, cell-free matrices deposited by bovine aortic endothelial cells (BAEC) were isolated. These matrices were produced under conditions in which the formation of the sprouting phenotype was permitted (controls) or inhibited (by the addition of exogenous xylosides). BAEC were then plated on these matrices and grown under conditions which promote sprouting. Sprouting proceeded normally on control matrices, whereas it was inhibited when the cells were grown on matrices deposited in the presence of xylosides. The composition of the permissive and inhibitory matrices was then analysed. Inhibitory matrices contained reduced levels of tenascin and increased levels of thrombospondin-1 by comparison to the permissive matrices. In contrast, no differences were detected in the relative levels of laminin. The roles of tenascin and thrombospondin-1 in endothelial sprouting were confirmed using specific antibodies. Immunolocalisation studies revealed the presence of both proteins in sprouting cells. Antibodies to tenascin inhibited the formation of sprouting cells on permissive matrices and on gelatin-coated dishes without affecting cell growth. Tenascin synthesis was increased when sprouting cells were present in the cultures. Antibodies to thrombospondin-1 stimulated sprouting on inhibitory matrices. These results suggest that the transition from a resting to a sprouting phenotype is promoted by tenascin and inhibited by thrombospondin-1.

  • © 1995 by Company of Biologists

REFERENCES

    1. Adams J. C. and
    2. Lawler J.
    (1993). The thrombospondin family. Curr. Biol 3, 188–190
    OpenUrlCrossRefPubMedWeb of Science
    1. Adams J. C. and
    2. Watt F. M.
    (1993). Regulation of development and differentiation by the extracellular matrix. Development 117, 1183–1198
    OpenUrlPubMedWeb of Science
    1. Ben Ezra D.,
    2. Griffin B. W.,
    3. Maftzir G. and
    4. Aharonov O.
    (1993). Thrombospondin and in vivo angiogenesis induced by basic fibroblast growth factor or lipopolysaccharide. Invest. Ophthalmol. Vis. Sci 34, 3601–3608
    OpenUrlAbstract/FREE Full Text
    1. Borsi L.,
    2. Carnemolla B.,
    3. Nicolo G.,
    4. Tanara G. and
    5. Zardi L.
    (1992). Expression of different tenascin isoforms in normal, hyperplastic and neoplastic human breast tissues. Int. J. Cancer 52, 688–692
    OpenUrlCrossRefPubMedWeb of Science
    1. Bourdon M. A.,
    2. Wikstrand C. J.,
    3. Furthmayr H.,
    4. Matthews T. J. and
    5. Bigner D. D.
    (1983). Human glioma-mesenchymal extracellular matrix antigen defined by monoclonal antibody. Cancer Res 43, 2796–2805
    OpenUrlAbstract/FREE Full Text
    1. Bristow J.,
    2. Tee M. K.,
    3. Gitelman S. E.,
    4. Mellon S. H. and
    5. Willer W. L.
    (1993). Tenascin-X: A novel extracellular matrix protein encoded by the human XB gene overlapping p450c21B. J. Cell Biol 122, 265–278
    OpenUrlAbstract/FREE Full Text
    1. Bronner-Fraser M.
    (1988). Distribution and function of tenascin during cranial neural crest development in the chick. J. Neurosci. Res 21, 135–147
    OpenUrlCrossRefPubMedWeb of Science
    1. Canfield A. E.,
    2. Schor A. M.,
    3. Schor S. L. and
    4. Grant M. E.
    (1986). The biosynthesis of extracellular matrix components by bovine retinal endothelial cells displaying distinctive morphological phenotypes. Biochem. J 235, 375–383
    OpenUrlAbstract/FREE Full Text
    1. Canfield A. E.,
    2. Boot-Handford R. P. and
    3. Schor A. M.
    (1990). Thrombospondin gene expression by endothelial cells in culture is modulated by cell proliferation, cell shape and the substratum. Biochem. J 268, 225–230
    OpenUrlAbstract/FREE Full Text
    1. Canfield A. E.,
    2. Allen T. D.,
    3. Grant M. E.,
    4. Schor S. L. and
    5. Schor A. M.
    (1990). Modulation of extracellular matrix biosynthesis by bovine retinal pericytes in vitro: effects of substratum and cell density. J. Cell. Sci 96, 159–169
    OpenUrlAbstract/FREE Full Text
    1. Canfield A. E.,
    2. Sutton A. B.,
    3. Hiscock D. R. R.,
    4. Gallagher J. T. and
    5. Schor A. M.
    (1994). Alpha-and beta-xylosides modulate the synthesis of fibronectin and thrombospondin by endothelial cells. Biochim. Biophys. Acta 1200, 249–258
    OpenUrlPubMed
    1. Chiquet M.,
    2. Vrucinic-Filipi N.,
    3. Schenk S.,
    4. Beck K. and
    5. Chiquet-Ehrismann R.
    (1991). Isolation of chick tenascin variants and fragments. Eur. J. Biochem 199, 379–388
    OpenUrlPubMedWeb of Science
    1. Chiquet M.
    (1992). Tenascin: an extracellular matrix protein involved in morphogenesis of epithelial organs. Kidney Int 41, 629–631
    OpenUrlPubMed
    1. Chiquet-Ehrismann R.,
    2. Mackie E. J.,
    3. Pearson C. A. and
    4. Sakakura T.
    (1986). Tenascin: an extracellular matrix protein involved in tissue interactions during fetal development and oncogenesis. Cell 47, 131–139
    OpenUrlCrossRefPubMedWeb of Science
    1. Choung C. M.,
    2. Crossin K. L. and
    3. Edleman G. M.
    (1987). Sequential expression and differential function of multiple adhesion molecules during the formation of cerebrellar cortical layers. J. Cell Biol 104, 331–342
    OpenUrlAbstract/FREE Full Text
    1. Church G. M. and
    2. Gilbert W.
    (1984). Genomic Sequencing. Proc. Nat. Acad. Sci. USA 81, 1991–1995
    OpenUrlAbstract/FREE Full Text
    1. Dardik R. and
    2. Lahav J.
    (1991). Cell-binding domain of endothelial cell thrombospondin: localisation to the 70kDa core fragment and determination of binding characteristics. Biochemistry 30, 9378–9386
    OpenUrlCrossRefPubMed
    1. Dugariczyk A.,
    2. Haron J. A.,
    3. Stone E. M.,
    4. Dennison O. E.,
    5. Rothblum K. N. and
    6. Schwarytz R. J.
    (1983). Cloning and sequencing of a deoxyribonucleic acid copy of glyceraldehyde-3-phosphate dehydrogenase messenger ribonucleic acid isolated from chicken muscle. Biochemistry 22, 1605–1613
    OpenUrlCrossRefPubMed
    1. Engvall E.,
    2. Earwicker D.,
    3. Haaparanta T.,
    4. Ruoslahti E. and
    5. Sanes J. R.
    (1990). Distribution and isolation of four laminin variants; tissue restricted distribution of heterodimers assembled from five different subunits. Cell Regul 1, 731–740
    OpenUrlPubMedWeb of Science
    1. Erickson H. P. and
    2. Bourdon M. A.
    (1989). Tenascin: an extracellular matrix protein prominent in specialised embryonic tissues and tumors. Annu. Rev. Cell Biol 5, 71–92
    OpenUrlCrossRefWeb of Science
    1. Erickson H. P.
    (1993). Tenascin-C, tenascin-R and tenascin-X: a family of talented proteins in search of functions. Curr. Opin. Cell Biol 5, 869–876
    OpenUrlCrossRefPubMed
    1. Frazier W. A.
    (1991). Thrombospondins. Curr. Opin. Cell Biol 3, 792–799
    OpenUrlCrossRefPubMed
    1. Good D. J.,
    2. Polverini P.,
    3. Rastinejad F.,
    4. Le Beau M. M.,
    5. Lemons R. S.,
    6. Frazier W. A. and
    7. Bouck N. P.
    (1990). A tumour suppressor dependent inhibitor of angiogenesis is immunologically and functionally indistinguishable from a fragment of thrombospondin. Proc. Nat. Acad. Sci. USA 87, 6624–6628
    OpenUrlAbstract/FREE Full Text
    1. Grant D. S.,
    2. Tashiro K.-T.,
    3. Segui-Real B.,
    4. Yamada Y.,
    5. Martin G. R. and
    6. Kleinman H. K.
    (1989). Two different laminin domains mediate the differentiation of human endothelial cells into capillary-like structures in vitro. Cell 58, 933–943
    OpenUrlCrossRefPubMedWeb of Science
    1. Grant D. S.,
    2. Kinsella J. L.,
    3. Fridman R.,
    4. Auerbach R.,
    5. Piasecki B. A.,
    6. Yamada Y.,
    7. Zain M. and
    8. Kleinman H. K.
    (1992). Interaction of endothelial cells with a laminin A chain peptide (SIKVAV) in vitro and induction of angiogenic behaviour in vivo. J. Cell. Physiol 153, 614–625
    OpenUrlCrossRefPubMedWeb of Science
    1. Hedin U.,
    2. Holm J. and
    3. Hansson G. K.
    (1991). Induction of tenascin in rat arterial injury. Am. J. Pathol 139, 649–656
    OpenUrlPubMedWeb of Science
    1. Ingber D. E. and
    2. Folkman J.
    (1989). Mechano-chemical switching between growth and differentiation during fibroblast growth factor-stimulatedangiogenesis in vitro: role of the extracellular matrix. J. Cell Biol 109, 317–330
    OpenUrlAbstract/FREE Full Text
    1. Ingber D. E. and
    2. Folkman J.
    (1989). How does extracellular matrix control capillary morphogenesis?. Cell 58, 803–805
    OpenUrlCrossRefPubMedWeb of Science
    1. Iruela-Arispe M. L.,
    2. Bornstein P. and
    3. Sage H.
    (1991). Thrombospondin exerts an anti-angiogenic effect on cord formation by endothelial cells in vitro. Proc. Nat. Acad. Sci. USA 88, 5026–5030
    OpenUrlAbstract/FREE Full Text
    1. Lawler J.,
    2. Derick L. H.,
    3. Connolly J. E.,
    4. Chen J. H. and
    5. Chao F. C.
    (1985). The structure of human platelet thrombospondin. J. Biol. Chem 260, 3762–3774
    OpenUrlAbstract/FREE Full Text
    1. Mackie E. J.,
    2. Halfter W. and
    3. Liverani D.
    (1988). Induction of tenascin in healing wounds. J. Cell Biol 107, 2757–2767
    OpenUrlAbstract/FREE Full Text
    1. Madri J. A. and
    2. Williams S. K.
    (1983). Capillary endotheial cell cultures: phenotypic modulation by matrix components. J. Cell Biol 97, 153–165
    OpenUrlAbstract/FREE Full Text
    1. Madri J. A.,
    2. Pratt B. M. and
    3. Tucker A. M.
    (1988). Phenotypic modulation of endothelial cells by transforming growth factor-depends upon the composition and organisation of the extracellular matrix. J. Cell Biol 106, 1375–1384
    OpenUrlAbstract/FREE Full Text
    1. Mignatti P.,
    2. Tsuboi R.,
    3. Robbins E. and
    4. Rifkin D. B.
    (1989). In vitro angiogenesis on the human amniotic membrane: requirements for basic fibroblast growth factor-induced proteinases. J. Cell Biol 108, 671–682
    OpenUrlAbstract/FREE Full Text
    1. Montesano R.,
    2. Orci L. and
    3. Vassalli P.
    (1983). In vitro rapid organisation of endothelial cells into capillary-like networks is promoted by collagen matrices. J. Cell Biol 97, 1648–1652
    OpenUrlAbstract/FREE Full Text
    1. Nathan C. and
    2. Sporn M.
    (1991). Cytokines in context. J. Cell Biol 113, 981–986
    OpenUrlFREE Full Text
    1. Nicosia R. F.,
    2. Bonanno E. and
    3. Smith M.
    (1993). Fibronectin promotes the elongation of microvessels during angiogenesis in vitro. J. Cell. Physiol 154, 654–661
    OpenUrlCrossRefPubMedWeb of Science
    1. Nicosia R. F. and
    2. Tuszynski G. P.
    (1994). Matrix-bound thrombospondin promotes angiogenesis in vitro. J. Cell Biol 124, 183–193
    OpenUrlAbstract/FREE Full Text
    1. Pepper M. S.,
    2. Belin D.,
    3. Montesano R.,
    4. Orci L. and
    5. Vassali J.-D.
    (1990). Transforming growth factor beta-1 modulates basic fibroblast growth factor-induced proteolytic and angiogenic properties of endothelial cells in vitro. J. Cell Biol 111, 743–755
    OpenUrlAbstract/FREE Full Text
    1. Risau W. and
    2. Lemmon V.
    (1988). Changes in the vascular extracellular matrix during embryonic vasculogenesis and angiogenesis. Dev. Biol 125, 441–450
    OpenUrlCrossRefPubMedWeb of Science
    1. Saga Y.,
    2. Yagi T.,
    3. Ikawa Y.,
    4. Sakakura T. and
    5. Aizawa S.
    (1992). Mice develop normally without tenascin. Genes Dev 6, 1821–1831
    OpenUrlAbstract/FREE Full Text
    1. Sakamoto N.,
    2. Iwahana M.,
    3. Tanaka N. G. and
    4. Ogada Y.
    (1991). Inhibition of angiogenesis and tumour growth by a synthetic laminin peptide, CDPGYIGSR-NH2. Cancer Res 51, 903–906
    OpenUrlAbstract/FREE Full Text
    1. Schor A. M.,
    2. Schor S. L. and
    3. Allen T. D.
    (1983). Effects of culture conditions on the proliferation, morphology and migration of bovine aortic endothelial cells. J. Cell Sci 62, 267–285
    OpenUrlAbstract/FREE Full Text
    1. Schor A. M.,
    2. Schor S. L. and
    3. Allen T. D.
    (1984). The synthesis of subendothelial matrix by bovine aortic endothelial cells in culture. Tissue and Cell 16, 677–691
    OpenUrlCrossRefPubMed
    1. Schor A. M. and
    2. Schor S. L.
    (1988). Inhibition of endothelial cell morphogenetic interactions in vitro by alpha-and beta-xylosides. In Vitro Cell Dev. Biol 24, 659–668
    OpenUrlPubMed
    1. Schor A. M.,
    2. Canfield A. E.,
    3. Sloan P. and
    4. Schor S. L.
    (1991). Differentiation of pericytes in culture is accompanied by changes in the extracellular matrix. In Vitro Cell. Dev. Biol 27, 651–659
    OpenUrlCrossRefWeb of Science
    1. Siri A.,
    2. Carnemolla B.,
    3. Saginati M.,
    4. Leprini A.,
    5. Casari G.,
    6. Baralle F. and
    7. Zardi L.
    (1991). Human tenascin: primary structure, pre-mRNA splicing patterns and localisation of the epitopes recognised by two monoclonal antibodies. Nucl. Acid Res 19, 525–531
    OpenUrlAbstract/FREE Full Text
    1. Sutton A. B.,
    2. Canfield A. E.,
    3. Schor S. L.,
    4. Grant M. E. and
    5. Schor A. M.
    (1991). The response of endothelial cells to TGF-1 is dependent upon cell shape, proliferative state and the nature of the substratum. J. Cell Sci 99, 777–787
    OpenUrlAbstract/FREE Full Text
    1. Tolsma S. S.,
    2. Volpert O. V.,
    3. Good D. J.,
    4. Frazier W. A.,
    5. Polverini P. J. and
    6. Bouck N.
    (1993). Peptides derived from two separate domains of the matrix protein thrombospondin-1 have anti-angiogenic activity. J. Cell Biol 122, 497–511
    OpenUrlAbstract/FREE Full Text
    1. Tryggvason K.
    (1993). The laminin family. Curr. Opin. Cell Biol 5, 877–882
    OpenUrlCrossRefPubMed
    1. Vernon R. B.,
    2. Angello J. C.,
    3. Iruela-Arispe M. L.,
    4. Lane T. F. and
    5. Sage E. H.
    (1992). Reorganisation of basement membrane matrices by cellular traction promotes the formation of cellular networks in vitro. Lab. Invest 66, 536–547
    OpenUrlPubMedWeb of Science
    1. Wakui S.,
    2. Furusato M.,
    3. Nikaido T.,
    4. Yokota K.,
    5. Sekiguchi J.,
    6. Ohmori K.,
    7. Kano Y. and
    8. Ushigome S.
    (1990). Ultrastructural localisation of fibronectin and laminin in human granulation tissue in relation to capillary development. Cell Struct. Funct 15, 201–210
    OpenUrlPubMed
    1. Whitby D. J.,
    2. Longaker M. T.,
    3. Harrison M. R.,
    4. Adzick N. S. and
    5. Ferguson M. W. J.
    (1991). Rapid epithelialisation of fetal wounds is associated with the early deposition of tenascin. J. Cell Sci 99, 583–586
    OpenUrlAbstract/FREE Full Text
    1. Wight T. N.,
    2. Raugi G. J.,
    3. Mumby S. M. and
    4. Bornstein P.
    (1985). Light microscopic immunolocalisation of thrombospondin in human tissues. J. Histochem. Cytochem 33, 295–302
    OpenUrlAbstract/FREE Full Text
    1. Yurchenco P. D.
    (1990). Assembly of basement membranes. Ann. NY Acad. Sci 580, 195–213
    OpenUrlCrossRefPubMed
Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

 Download PDF

Email

Thank you for your interest in spreading the word on Journal of Cell Science.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Evidence that tenascin and thrombospondin-1 modulate sprouting of endothelial cells
(Your Name) has sent you a message from Journal of Cell Science
(Your Name) thought you would like to see the Journal of Cell Science web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Journal Articles
Evidence that tenascin and thrombospondin-1 modulate sprouting of endothelial cells
A.E. Canfield, A.M. Schor
Journal of Cell Science 1995 108: 797-809;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Journal Articles
Evidence that tenascin and thrombospondin-1 modulate sprouting of endothelial cells
A.E. Canfield, A.M. Schor
Journal of Cell Science 1995 108: 797-809;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
  • Info & metrics
  • PDF

Related articles

Cited by...

More in this TOC section

  • Involvement of actin filaments and integrins in the binding step in collagen phagocytosis by human fibroblasts
  • University administration
  • Integrin cytoplasmic domain-binding proteins
Show more Journal Articles

Similar articles

Other journals from The Company of Biologists

Development

Journal of Experimental Biology

Disease Models & Mechanisms

Biology Open

Advertisement

Introducing FocalPlane’s new Community Manager, Esperanza Agullo-Pascual

We are pleased to welcome Esperanza to the Journal of Cell Science team. The new Community Manager for FocalPlane, Esperanza is joining us from the Microscopy Core at Mount Sinai School of Medicine. Find out more about Esperanza in her introductory post over on FocalPlane.


New funding scheme supports sustainable events

As part of our Sustainable Conferencing Initiative, we are pleased to announce funding for organisers that seek to reduce the environmental footprint of their event. The next deadline to apply for a Scientific Meeting grant is 26 March 2021.


Read & Publish participation continues to grow

"Alongside pre-printing for early documentation of work, such mechanisms are particularly helpful for early-career researchers like me.”

Dr Chris MacDonald (University of York) shares his experience of publishing Open Access as part of our growing Read & Publish initiative. We now have over 150 institutions in 15 countries and four library consortia taking part – find out more and view our full list of participating institutions.


Cell scientist to watch: Romain Levayer

In an interview, Romain Levayer talks about starting his own lab, his love for preprints and his experience of balancing parenting with his research goals.


Live lactating mammary tissue

In a stunning video, Stewart et al. demonstrate warping of the alveolar unit due to basal cell-generated force as part of their recent work investigating roles for mechanically activated ion channels in lactation and involution.

Visit our YouTube channel to watch more videos from JCS, our sister journals and the Company.


JCS and COVID-19

For more information on measures Journal of Cell Science is taking to support the community during the COVID-19 pandemic, please see here.

If you have any questions or concerns, please do not hestiate to contact the Editorial Office.

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Interviews
  • Sign up for alerts

About us

  • About Journal of Cell Science
  • Editors and Board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For Authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Fast-track manuscripts
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • JCS Prize
  • Manuscript transfer network
  • Biology Open transfer

Journal Info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contacts

  • Contact JCS
  • Subscriptions
  • Advertising
  • Feedback

Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992